DIMA_DICDI
ID DIMA_DICDI Reviewed; 1230 AA.
AC Q54XG7; Q6T872;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 02-MAY-2006, sequence version 2.
DT 25-MAY-2022, entry version 100.
DE RecName: Full=Basic-leucine zipper transcription factor A;
DE AltName: Full=DIF insensitive mutant A;
GN Name=dimA; Synonyms=bzpA; ORFNames=DDB_G0278971;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DEVELOPMENTAL STAGE, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=AX4;
RX PubMed=14729573; DOI=10.1242/dev.00939;
RA Thompson C.R.L., Fu Q., Buhay C., Kay R.R., Shaulsky G.;
RT "A bZIP/bRLZ transcription factor required for DIF signaling in
RT Dictyostelium.";
RL Development 131:513-523(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP DISRUPTION PHENOTYPE, FUNCTION, INTERACTION WITH DIMB, DEVELOPMENTAL STAGE,
RP AND SUBCELLULAR LOCATION.
RX PubMed=16410410; DOI=10.1242/dev.02240;
RA Huang E., Blagg S.L., Keller T., Katoh M., Shaulsky G., Thompson C.R.L.;
RT "bZIP transcription factor interactions regulate DIF responses in
RT Dictyostelium.";
RL Development 133:449-458(2006).
CC -!- FUNCTION: Transcriptional regulator involved in DIF-1 signaling. DIF-1
CC (Differentiation Inducing Factor-1) is a signal molecule involved in
CC the differentiation of pstO (prestalk-O) cells. Functions as both an
CC activator of prestalk gene expression and repressor of prespore gene
CC expression. {ECO:0000269|PubMed:14729573, ECO:0000269|PubMed:16410410}.
CC -!- SUBUNIT: Binds DNA as a dimer (By similarity). Heterodimerizes with
CC dimB; in vitro. Also able to form homodimer; in vitro. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00978,
CC ECO:0000269|PubMed:16410410}. Note=In response to DIF-1, it accumulates
CC rapidly in the nucleus. Nuclear accumulation is dependent on dimB.
CC -!- DEVELOPMENTAL STAGE: Maximum levels of expression in prespore cells,
CC but also expressed in prestalk cells at the slug stage. Developmentally
CC regulated with levels peaking at culmination.
CC {ECO:0000269|PubMed:14729573, ECO:0000269|PubMed:16410410}.
CC -!- DISRUPTION PHENOTYPE: Defective in DIF biosynthesis and in DIF-1-
CC induced stalk-cell differentiation. Exhibits morphological and cell
CC type differentiation defects. DimA and dimB double mutant has the same
CC phenotype. {ECO:0000269|PubMed:14729573, ECO:0000269|PubMed:16410410}.
CC -!- SIMILARITY: Belongs to the bZIP family. {ECO:0000305}.
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DR EMBL; AY428796; AAR97288.1; -; Genomic_DNA.
DR EMBL; AAFI02000026; EAL67907.2; -; Genomic_DNA.
DR RefSeq; XP_641883.2; XM_636791.2.
DR AlphaFoldDB; Q54XG7; -.
DR SMR; Q54XG7; -.
DR STRING; 44689.DDB0216189; -.
DR PaxDb; Q54XG7; -.
DR EnsemblProtists; EAL67907; EAL67907; DDB_G0278971.
DR GeneID; 8621809; -.
DR KEGG; ddi:DDB_G0278971; -.
DR dictyBase; DDB_G0278971; dimA.
DR eggNOG; ENOG502RB48; Eukaryota.
DR HOGENOM; CLU_267808_0_0_1; -.
DR InParanoid; Q54XG7; -.
DR OMA; TITGIWG; -.
DR PRO; PR:Q54XG7; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IDA:dictyBase.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; IPI:dictyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:dictyBase.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0006935; P:chemotaxis; IMP:dictyBase.
DR GO; GO:0043944; P:negative regulation of asexual sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR GO; GO:0031287; P:positive regulation of sorocarp stalk cell differentiation; IMP:dictyBase.
DR GO; GO:0012501; P:programmed cell death; IMP:dictyBase.
DR GO; GO:0045595; P:regulation of cell differentiation; IMP:dictyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:1903013; P:response to differentiation-inducing factor 1; IMP:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IMP:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
DR InterPro; IPR004827; bZIP.
DR InterPro; IPR046347; bZIP_sf.
DR SMART; SM00338; BRLZ; 1.
DR SUPFAM; SSF57959; SSF57959; 1.
DR PROSITE; PS50217; BZIP; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Differentiation; DNA-binding; Nucleus; Reference proteome;
KW Sporulation; Transcription; Transcription regulation.
FT CHAIN 1..1230
FT /note="Basic-leucine zipper transcription factor A"
FT /id="PRO_0000328625"
FT DOMAIN 555..618
FT /note="bZIP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 65..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 270..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 422..578
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 556..586
FT /note="Basic motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 590..604
FT /note="Leucine-zipper"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00978"
FT REGION 772..869
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1025..1230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 728..753
FT /evidence="ECO:0000255"
FT COMPBIAS 293..310
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 422..454
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 465..517
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 772..830
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 831..845
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1025..1210
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 365
FT /note="A -> T (in Ref. 1; AAR97288)"
FT /evidence="ECO:0000305"
FT CONFLICT 504
FT /note="Missing (in Ref. 1; AAR97288)"
FT /evidence="ECO:0000305"
FT CONFLICT 677..680
FT /note="ARLA -> VS (in Ref. 1; AAR97288)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1230 AA; 140627 MW; 0785C9B5977753B8 CRC64;
MDSDNWNFQN NPYFQDYNIG NPNNNTNGIN GSSNGTMNLS NSASLFLNKN LGNSDFQPFS
LQPSLYLSNS SNNTNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNIINNN TDEFVFKKIS
ANNLQTPTSP PKDLGFIQTI LQHQQELFQK KLSESGVTNL QPDQIQFMFM QEQQKYLTSL
NGNDNTLPPL ENNFSFQHVN NNNLTSPPQQ QQQQQQQQQQ QQHQQHQQHQ QHQQQQQQQQ
QQQQQQHQHQ QQQQQQQQQQ QQQQQQQQQQ QLKQQQPQQH PIQSPQPIQS PTPSPSLQQH
QTYSYTPSTQ QTSLQQLQQQ QMQQLLQMSP QHQQQHNQIQ QQQAAAAAAA LLQQQLAQQQ
AQQLAQQQQL AQQQLAQQQQ QQQQQHVQSL HQAQVQQAHM SQLQQQQLAQ QAFQQAQQQQ
FHQQNIQQHQ NQNQQQLQLP QPQQQQHKST PPTQNTPPVK SPAPQTPTLT TNGKGSKSTP
PTTTTTTTTT TSSSSSSSSS SSSSKKKTSN KKTGNLQVPP TPPHHHHHHH NNHHHHHSEG
FSDENDEEFI DENEDKSKNK SRSSQNIASR NYRQRKKDHI SEVEFKVQQL SLENERLKQE
NHLLKKGDLG DVMRPDFDFQ QVLLESQKLM SQLQDAVNKQ DHATIENLLQ LYYFASQLRT
TVVEREVEKI VHPYTQARLA VMGYRSNAET SILLRPFSTN LWWPKYADEV GLTEEQRKAS
DILWTDHLKI DMELRTERDQ LDREIKELFL KKIVSHGTKR PELLFERETL STFNSESDYP
SSPSSASNSS NSPPTSSPTI ITPPDSPLTN NQNNQNNNQM INSNSNNSNN NSHHHHHHHH
SHLHGHSPYP VPNGTVHPPP TTAVSEHNKP IELSELLDVT RKLEMLKKNF VKHRNLICDT
DLVLSTILTP FQHAKLILRL NSVTCYDFSI VDTITGIWGS INSTLKEGSF IQNLMPDQDG
KFEVLEKFKQ NQLLDLKGQR PIKLESLQRT YEKLYQTVLG SESPNCQFVK QADYQHQNLL
TASPNYTNSP LITSSPSQLT PNSPRPPLDI SNNNNNNNNN NNNNNNNNNN NNNNNNNNNN
NNNNNNNNNN NNNNNNNSNN NNNTNNNNNN YNNNGNNNNG NNNNGNNNNG NNGNNGNNSN
NSNNGNINSV DIQALHQQQK QNLQPQQLQQ NQQLQQNQNT PPQQSQQVCS PSSPMTPELN
KQPEIVGGDN TNKKNKSKKY QWYSYKTPNI
