ID   DING_BACSU              Reviewed;         931 AA.
AC   P54394;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 149.
DE   RecName: Full=3'-5' exonuclease DinG {ECO:0000255|HAMAP-Rule:MF_02206};
DE            EC=3.1.-.- {ECO:0000255|HAMAP-Rule:MF_02206};
GN   Name=dinG {ECO:0000255|HAMAP-Rule:MF_02206}; OrderedLocusNames=BSU22400;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC   3610 / NRRL NRS-744 / VKM B-501;
RX   PubMed=8760912; DOI=10.1099/13500872-142-8-2005;
RA   Sorokin A.V., Azevedo V., Zumstein E., Galleron N., Ehrlich S.D.,
RA   Serror P.;
RT   "Sequence analysis of the Bacillus subtilis chromosome region between the
RT   serA and kdg loci cloned in a yeast artificial chromosome.";
RL   Microbiology 142:2005-2016(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
CC   -!- FUNCTION: 3'-5' exonuclease. {ECO:0000255|HAMAP-Rule:MF_02206}.
CC   -!- SIMILARITY: Belongs to the helicase family. DinG subfamily. Type 2 sub-
CC       subfamily. {ECO:0000255|HAMAP-Rule:MF_02206}.
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DR   EMBL; L47709; AAB38451.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14156.1; -; Genomic_DNA.
DR   PIR; G69615; G69615.
DR   RefSeq; NP_390121.1; NC_000964.3.
DR   RefSeq; WP_004398920.1; NZ_JNCM01000036.1.
DR   AlphaFoldDB; P54394; -.
DR   SMR; P54394; -.
DR   IntAct; P54394; 2.
DR   STRING; 224308.BSU22400; -.
DR   PaxDb; P54394; -.
DR   PRIDE; P54394; -.
DR   EnsemblBacteria; CAB14156; CAB14156; BSU_22400.
DR   GeneID; 939031; -.
DR   KEGG; bsu:BSU22400; -.
DR   PATRIC; fig|224308.179.peg.2444; -.
DR   eggNOG; COG0847; Bacteria.
DR   eggNOG; COG1199; Bacteria.
DR   InParanoid; P54394; -.
DR   OMA; VVTNHAM; -.
DR   PhylomeDB; P54394; -.
DR   BioCyc; BSUB:BSU22400-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0008408; F:3'-5' exonuclease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR   GO; GO:0003678; F:DNA helicase activity; IBA:GO_Central.
DR   GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR   GO; GO:0016818; F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides; IEA:InterPro.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   Gene3D; 3.30.420.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 2.
DR   HAMAP; MF_02206; DinG_exonucl; 1.
DR   InterPro; IPR006555; ATP-dep_Helicase_C.
DR   InterPro; IPR006310; DinG.
DR   InterPro; IPR045028; DinG/Rad3-like.
DR   InterPro; IPR006054; DnaQ.
DR   InterPro; IPR013520; Exonuclease_RNaseT/DNA_pol3.
DR   InterPro; IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
DR   InterPro; IPR006935; Helicase/UvrB_N.
DR   InterPro; IPR014001; Helicase_ATP-bd.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR012337; RNaseH-like_sf.
DR   InterPro; IPR036397; RNaseH_sf.
DR   PANTHER; PTHR11472; PTHR11472; 1.
DR   Pfam; PF13307; Helicase_C_2; 1.
DR   Pfam; PF04851; ResIII; 1.
DR   Pfam; PF00929; RNase_T; 1.
DR   SMART; SM00487; DEXDc; 1.
DR   SMART; SM00479; EXOIII; 1.
DR   SMART; SM00491; HELICc2; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   SUPFAM; SSF53098; SSF53098; 1.
DR   TIGRFAMs; TIGR01407; dinG_rel; 1.
DR   TIGRFAMs; TIGR00573; dnaq; 1.
DR   PROSITE; PS51193; HELICASE_ATP_BIND_2; 1.
DR   PROSITE; PS51194; HELICASE_CTER; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Exonuclease; Hydrolase; Nuclease; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..931
FT                   /note="3'-5' exonuclease DinG"
FT                   /id="PRO_0000101995"
FT   DOMAIN          7..162
FT                   /note="Exonuclease"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02206"
FT   DOMAIN          250..510
FT                   /note="Helicase ATP-binding"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02206"
FT   DOMAIN          741..897
FT                   /note="Helicase C-terminal"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02206"
FT   MOTIF           462..465
FT                   /note="DEAH box"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02206"
FT   BINDING         284..291
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_02206"
SQ   SEQUENCE   931 AA;  106032 MW;  5E2DE3531C18A8C4 CRC64;
     MNKQRFVVID VETTGNSPKK GDKIIQIAAV VIENGQITER FSKYINPNKS IPAFIEQLTG
     ISNQMVENEQ PFEAVAEEVF QLLDGAYFVA HNIHFDLGFV KYELHKAGFQ LPDCEVLDTV
     ELSRIVFPGF EGYKLTELSE ELQLRHDQPH RADSDAEVTG LIFLEILEKL RQLPYPTLKQ
     LRRLSQHFIS DLTHLLDMFI NENRHTEIPG YTRFSSFSVR EPEAIDVRIN EDENFSFEIE
     SWEAGNEKAL SELMPGYEKR DGQMMMMREV ADAFANREHA LIEAPPGIGK TIGYLIPAAL
     FAKKSKKPVI ISTYSTLLQQ QILTKDLPIV QDLFPFPVTA AILKGQSHYL CLYKFEQVLH
     EEDDNYDAVL TKAQLLVWLT ETNTGDVAEL NLPSGGKLLW DRLAYDDDSY KRSRSEHVIG
     FYERAKQIAM RSDLVITNHS LLLTDEGSHK KRLPESGTFI IDEAHHFERA ASEHLGKRAT
     YIELHTKLSR IGTLKEQGLL KKMRQLFQRN SLPVDSFFEL EEWLQHVQAE SDAFFSSVHS
     FVKRRKPKED LNRLVFKVNK ESQDKSWSIL TDGAERLCSM LTHLQQLFEA QSSLMEKHLK
     GMKSKTVFLA DEYQRSMKGL QHYCQTLQKL FFGSDDDEAV WIEIDAKGAK NAVAIYAQPL
     EPGELLADQF FARKNSVVLT SATLTVEGSF QFMIERLGLS DFFPRTMRIE SPFSYDERMQ
     VMIPKEMKSI QDTGQPEFIQ DTARYIELMA KEKQPKILVL FTSHDMLKKV HQELKHNMSA
     SGIQLLAQGI TGGSPGKLMK TFKTSNQAIL LGTNHFWEGV DFPGDELTTV MIVRLPFRSP
     DHPLHAAKCE LARKKGKNPF QTVSLPEAVL TFRQGIGRLL RSAGDKGTII ILDRRIKTAG
     YGRLFLDALP TTSVSEMTDS ELEAYVAGEK E