ID   DINJ_ECOBD              Reviewed;          86 AA.
AC   A0A140ND86;
DT   05-JUL-2017, integrated into UniProtKB/Swiss-Prot.
DT   11-MAY-2016, sequence version 1.
DT   25-MAY-2022, entry version 30.
DE   RecName: Full=Antitoxin DinJ;
GN   Name=dinJ; OrderedLocusNames=ECBD_3396;
OS   Escherichia coli (strain B / BL21-DE3).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=469008;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=B / BL21-DE3;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., LaButti K.M., Clum A., Larimer F.,
RA   Land M., Hauser L., Kyrpides N., Anderson I., Sorek R., Rubin E.;
RT   "Complete sequence of Escherichia coli BL21(DE3).";
RL   Submitted (JUL-2009) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0007744|PDB:4ML0}
RP   X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) IN COMPLEX WITH YAFQ, FUNCTION,
RP   SUBUNIT, DNA-BINDING, AND MUTAGENESIS OF ARG-8; LYS-16; 31-SER--ARG-35;
RP   PHE-49 AND 59-ILE--ILE-62.
RC   STRAIN=B / BL21-DE3;
RX   PubMed=24923448; DOI=10.1074/jbc.m114.559773;
RA   Liang Y., Gao Z., Wang F., Zhang Y., Dong Y., Liu Q.;
RT   "Structural and functional characterization of Escherichia coli toxin-
RT   antitoxin complex DinJ-YafQ.";
RL   J. Biol. Chem. 289:21191-21202(2014).
CC   -!- FUNCTION: Antitoxin component of a type II toxin-antitoxin (TA) system
CC       (PubMed:24923448). A labile antitoxin that counteracts the effect of
CC       cognate toxin YafQ (PubMed:24923448). The YafQ-DinJ heterotetramer
CC       binds the consensus sequence 5'-TTTGAGCTACA-3' in the dinJ promoter;
CC       DinJ also binds DNA but not as well as the YafQ-DinJ complex
CC       (PubMed:24923448). Binding to the dinJ represses expression of the
CC       promoter (By similarity). {ECO:0000250|UniProtKB:Q47150,
CC       ECO:0000269|PubMed:24923448}.
CC   -!- SUBUNIT: Probably a dimer in solution, forms a heterotetramer with
CC       antitoxin DinJ, with 2 YafQ-DinJ dimers associated via the N-terminus
CC       of the DinJ antitoxins (YafQ-(DinJ)2-YafQ) (PubMed:24923448).
CC       {ECO:0000269|PubMed:24923448}.
CC   -!- SIMILARITY: Belongs to the RelB/DinJ antitoxin family. {ECO:0000305}.
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DR   EMBL; CP001665; ACT30396.1; -; Genomic_DNA.
DR   RefSeq; WP_000729704.1; NZ_CP053602.1.
DR   PDB; 4ML0; X-ray; 2.10 A; A/C/E/G/I/K/M/O=1-86.
DR   PDBsum; 4ML0; -.
DR   AlphaFoldDB; A0A140ND86; -.
DR   SMR; A0A140ND86; -.
DR   STRING; 469008.B21_00225; -.
DR   GeneID; 66671455; -.
DR   KEGG; ebd:ECBD_3396; -.
DR   PATRIC; fig|469008.15.peg.225; -.
DR   eggNOG; COG3077; Bacteria.
DR   HOGENOM; CLU_154558_12_2_6; -.
DR   OMA; FEVRIPN; -.
DR   Proteomes; UP000002032; Chromosome.
DR   GO; GO:0000987; F:cis-regulatory region sequence-specific DNA binding; IEA:InterPro.
DR   GO; GO:0015643; F:toxic substance binding; IEA:InterPro.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR   GO; GO:0044010; P:single-species biofilm formation; IEA:InterPro.
DR   Gene3D; 1.10.1220.10; -; 1.
DR   InterPro; IPR013321; Arc_rbn_hlx_hlx.
DR   InterPro; IPR026262; DinJ.
DR   InterPro; IPR007337; RelB/DinJ.
DR   PANTHER; PTHR38781; PTHR38781; 1.
DR   Pfam; PF04221; RelB; 1.
DR   PIRSF; PIRSF003108; DinJ; 1.
DR   TIGRFAMs; TIGR02384; RelB_DinJ; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Repressor; Toxin-antitoxin system;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..86
FT                   /note="Antitoxin DinJ"
FT                   /id="PRO_0000440936"
FT   MUTAGEN         8
FT                   /note="R->A: Does not bind to dinJ promoter; when
FT                   associated with A-16."
FT                   /evidence="ECO:0000269|PubMed:24923448"
FT   MUTAGEN         16
FT                   /note="K->A: Does not bind to dinJ promoter; when
FT                   associated with A-8."
FT                   /evidence="ECO:0000269|PubMed:24923448"
FT   MUTAGEN         31..35
FT                   /note="SDLVR->ADLVA: Does not bind to dinJ promoter."
FT                   /evidence="ECO:0000269|PubMed:24923448"
FT   MUTAGEN         49
FT                   /note="F->A: No effect in vivo, in vitro forms a complex
FT                   with toxin YafQ which is not able to cleave RNA, i.e. does
FT                   not release YafQ. YafQ-DinJ complex binds DNA."
FT                   /evidence="ECO:0000269|PubMed:24923448"
FT   MUTAGEN         59..62
FT                   /note="IQSI->NQSN: No effect in vivo, in vitro forms a
FT                   complex with toxin YafQ which is still able to cleave RNA,
FT                   i.e. has lost antitoxin activity. YafQ-DinJ complex binds
FT                   DNA."
FT                   /evidence="ECO:0000269|PubMed:24923448"
FT   STRAND          4..10
FT                   /evidence="ECO:0007829|PDB:4ML0"
FT   HELIX           13..24
FT                   /evidence="ECO:0007829|PDB:4ML0"
FT   TURN            25..27
FT                   /evidence="ECO:0007829|PDB:4ML0"
FT   HELIX           30..44
FT                   /evidence="ECO:0007829|PDB:4ML0"
FT   HELIX           56..67
FT                   /evidence="ECO:0007829|PDB:4ML0"
FT   TURN            68..70
FT                   /evidence="ECO:0007829|PDB:4ML0"
FT   STRAND          71..76
FT                   /evidence="ECO:0007829|PDB:4ML0"
FT   HELIX           77..84
FT                   /evidence="ECO:0007829|PDB:4ML0"
SQ   SEQUENCE   86 AA;  9392 MW;  9B91EA370D90B3E2 CRC64;
     MAANAFVRAR IDEDLKNQAA DVLAGMGLTI SDLVRITLTK VAREKALPFD LREPNQLTIQ
     SIKNSEAGVD VHKAKDADDL FDKLGI