ID   DIPD_PROTE              Reviewed;         101 AA.
AC   P18684;
DT   01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=Diptericin-D;
DE   Flags: Precursor;
OS   Protophormia terraenovae (Northern blowfly) (Lucilia terraenovae).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Oestroidea;
OC   Calliphoridae; Chrysomyinae; Protophormia.
OX   NCBI_TaxID=34676;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RC   TISSUE=Larval fat body;
RX   PubMed=2544427; DOI=10.1111/j.1432-1033.1989.tb14848.x;
RA   Reichhart J.-M., Essrich M., Dimarcq J.-L., Hoffmann D., Hoffmann J.A.,
RA   Lagueux M.;
RT   "Insect immunity. Isolation of cDNA clones corresponding to diptericin, an
RT   inducible antibacterial peptide from Phormia terranovae (Diptera).
RT   Transcriptional profiles during immunization.";
RL   Eur. J. Biochem. 182:423-427(1989).
RN   [2]
RP   PROTEIN SEQUENCE OF 19-100, GLYCOSYLATION AT THR-28 AND THR-72, AND
RP   AMIDATION AT PHE-100.
RX   PubMed=7779781; DOI=10.1021/bi00022a012;
RA   Bulet P., Hegy G., Lambert J., van Dorsselaer A., Hoffmann J.A., Hetru C.;
RT   "Insect immunity. The inducible antibacterial peptide diptericin carries
RT   two O-glycans necessary for biological activity.";
RL   Biochemistry 34:7394-7400(1995).
RN   [3]
RP   GLYCOSYLATION AT THR-28 AND THR-72.
RX   PubMed=9177700; DOI=10.1006/abio.1997.2083;
RA   Uttenweiler-Joseph S., Moniatte M., Lambert J., van Dorsselaer A.,
RA   Bulet P.;
RT   "A matrix-assisted laser desorption ionization time-of-flight mass
RT   spectrometry approach to identify the origin of the glycan heterogeneity of
RT   diptericin, an O-glycosylated antibacterial peptide from insects.";
RL   Anal. Biochem. 247:366-375(1997).
CC   -!- FUNCTION: Has activity against E.coli.
CC   -!- INDUCTION: By bacterial infection. {ECO:0000269|PubMed:2544427}.
CC   -!- MISCELLANEOUS: There seems to be a family of diptericin in
CC       protophormia. Diptericin A is the predominant member.
CC   -!- SIMILARITY: Belongs to the attacin/sarcotoxin-2 family. {ECO:0000305}.
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DR   EMBL; X15851; CAB57822.1; -; mRNA.
DR   PIR; S04634; S04634.
DR   AlphaFoldDB; P18684; -.
DR   GlyConnect; 121; 3 O-Linked glycans (2 sites).
DR   GO; GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Glycoprotein; Immunity; Innate immunity; Signal.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000269|PubMed:7779781"
FT   CHAIN           19..100
FT                   /note="Diptericin-D"
FT                   /id="PRO_0000004907"
FT   MOD_RES         100
FT                   /note="Phenylalanine amide"
FT                   /evidence="ECO:0000269|PubMed:7779781"
FT   CARBOHYD        28
FT                   /note="O-linked (GalNAc...) threonine"
FT                   /evidence="ECO:0000269|PubMed:7779781,
FT                   ECO:0000269|PubMed:9177700"
FT                   /id="CAR_000126"
FT   CARBOHYD        72
FT                   /note="O-linked (GalNAc...) threonine"
FT                   /evidence="ECO:0000269|PubMed:7779781,
FT                   ECO:0000269|PubMed:9177700"
FT                   /id="CAR_000127"
FT   VARIANT         9
FT                   /note="I -> L"
FT   VARIANT         35
FT                   /note="P -> T"
SQ   SEQUENCE   101 AA;  10731 MW;  60E4DE34DF0E8C5A CRC64;
     MKLFYLLVIC ALSLAVMADE KPKLILPTPA PPNLPQLVGG GGGNRKDGFG VSVDAHQKVW
     TSDNGRHSIG VTPGYSQHLG GPYGNSRPDY RIGAGYSYNF G