DIR12_ARATH
ID DIR12_ARATH Reviewed; 185 AA.
AC O82498;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Dirigent protein 12;
DE Short=AtDIR12;
DE Flags: Precursor;
GN Name=DIR12; Synonyms=DP1; OrderedLocusNames=At4g11180; ORFNames=F2P3.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA Ralph S.G., Jancsik S., Bohlmann J.;
RT "Dirigent proteins in conifer defense II: Extended gene discovery,
RT phylogeny, and constitutive and stress-induced gene expression in spruce
RT (Picea spp.).";
RL Phytochemistry 68:1975-1991(2007).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=20023150; DOI=10.1104/pp.109.148031;
RA Matsuda F., Hirai M.Y., Sasaki E., Akiyama K., Yonekura-Sakakibara K.,
RA Provart N.J., Sakurai T., Shimada Y., Saito K.;
RT "AtMetExpress development: a phytochemical atlas of Arabidopsis
RT development.";
RL Plant Physiol. 152:566-578(2010).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=23115000; DOI=10.1007/s11103-012-9984-0;
RA Esfandiari E., Jin Z., Abdeen A., Griffiths J.S., Western T.L.,
RA Haughn G.W.;
RT "Identification and analysis of an outer-seed-coat-specific promoter from
RT Arabidopsis thaliana.";
RL Plant Mol. Biol. 81:93-104(2013).
CC -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC radical-coupling reaction, yielding optically active lignans from two
CC molecules of coniferyl alcohol in the biosynthesis of lignans,
CC flavonolignans, and alkaloids and thus plays a central role in plant
CC secondary metabolism (By similarity). Required for seed accumulation of
CC neolignans. {ECO:0000250, ECO:0000269|PubMed:20023150}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Seed coat specific expression.
CC {ECO:0000269|PubMed:20023150, ECO:0000269|PubMed:23115000}.
CC -!- DISRUPTION PHENOTYPE: Lacks seed-specific neolignans such as 3-{4-[2-
CC hydroxy-2-(4-hexosyloxy- 3-methoxyphenyl)-1-hydroxymethylethoxy]-3,5-
CC di-methoxyphenyl}acryloylcholine. {ECO:0000269|PubMed:20023150}.
CC -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC {ECO:0000305}.
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DR EMBL; AF080120; AAC35542.1; -; Genomic_DNA.
DR EMBL; AL049876; CAB43053.1; -; Genomic_DNA.
DR EMBL; AL161531; CAB81219.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82982.1; -; Genomic_DNA.
DR EMBL; BT004016; AAO42052.1; -; mRNA.
DR PIR; T01914; T01914.
DR RefSeq; NP_192857.1; NM_117189.2.
DR AlphaFoldDB; O82498; -.
DR SMR; O82498; -.
DR STRING; 3702.AT4G11180.1; -.
DR PaxDb; O82498; -.
DR PRIDE; O82498; -.
DR ProteomicsDB; 222145; -.
DR EnsemblPlants; AT4G11180.1; AT4G11180.1; AT4G11180.
DR GeneID; 826720; -.
DR Gramene; AT4G11180.1; AT4G11180.1; AT4G11180.
DR KEGG; ath:AT4G11180; -.
DR Araport; AT4G11180; -.
DR TAIR; locus:2136118; AT4G11180.
DR eggNOG; ENOG502RXV9; Eukaryota.
DR HOGENOM; CLU_087111_0_0_1; -.
DR InParanoid; O82498; -.
DR OMA; WVAWTLV; -.
DR OrthoDB; 1498667at2759; -.
DR PhylomeDB; O82498; -.
DR PRO; PR:O82498; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; O82498; baseline and differential.
DR Genevisible; O82498; AT.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009807; P:lignan biosynthetic process; IMP:TAIR.
DR GO; GO:0009699; P:phenylpropanoid biosynthetic process; IMP:TAIR.
DR Gene3D; 2.40.480.10; -; 1.
DR InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR InterPro; IPR004265; Dirigent.
DR PANTHER; PTHR46442; PTHR46442; 1.
DR Pfam; PF03018; Dirigent; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..185
FT /note="Dirigent protein 12"
FT /id="PRO_0000422843"
FT CARBOHYD 56
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 37..184
FT /evidence="ECO:0000250"
SQ SEQUENCE 185 AA; 20832 MW; 2A8DDB2427503156 CRC64;
MTNQIYKQVF SFFLSVLLLQ SSTVSYVPKS FDLKKPCKHF VLYLHNIAYD GDNAANATAA
TIVKPLGLGD HSFGELIIIN NPVTLDQNYL SKPVARAQGF YFYNMKTNYN AWVAWTLVFN
STKHKGTFTI MDANPFGLQP ARDLSIVGGT GDFLMTRGIA TFKTKLTQGS KYFCVEMNIK
LYECY
