DIR13_ARATH
ID DIR13_ARATH Reviewed; 184 AA.
AC Q9T017;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Dirigent protein 13;
DE Short=AtDIR13;
DE Flags: Precursor;
GN Name=DIR13; OrderedLocusNames=At4g11190; ORFNames=T22B4.170;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA Ralph S.G., Jancsik S., Bohlmann J.;
RT "Dirigent proteins in conifer defense II: Extended gene discovery,
RT phylogeny, and constitutive and stress-induced gene expression in spruce
RT (Picea spp.).";
RL Phytochemistry 68:1975-1991(2007).
RN [5]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=22854967; DOI=10.1074/jbc.m112.387423;
RA Kim K.-W., Moinuddin S.G.A., Atwell K.M., Costa M.A., Davin L.B.,
RA Lewis N.G.;
RT "Opposite stereoselectivities of dirigent proteins in Arabidopsis and
RT schizandra species.";
RL J. Biol. Chem. 287:33957-33972(2012).
CC -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC radical-coupling reaction, yielding optically active lignans from two
CC molecules of coniferyl alcohol in the biosynthesis of lignans,
CC flavonolignans, and alkaloids and thus plays a central role in plant
CC secondary metabolism. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in root vasculature and meristems,
CC cotyledons, flowers, siliques, and leaf trichomes. Localized in the
CC interfascicular/vascular cambia and developing xylem.
CC {ECO:0000269|PubMed:22854967}.
CC -!- DEVELOPMENTAL STAGE: In flowers, expressed in the vasculature of
CC sepals, petals, stamen filaments, and the gynoecium stylar region. In
CC siliques, observed in the stigmatic region and epidermal layers.
CC {ECO:0000269|PubMed:22854967}.
CC -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC {ECO:0000305}.
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DR EMBL; AL049876; CAB43054.1; -; Genomic_DNA.
DR EMBL; AL161531; CAB81220.1; -; Genomic_DNA.
DR EMBL; CP002687; AEE82983.1; -; Genomic_DNA.
DR EMBL; BT009718; AAP88352.1; -; mRNA.
DR PIR; T08198; T08198.
DR RefSeq; NP_192858.1; NM_117190.3.
DR AlphaFoldDB; Q9T017; -.
DR SMR; Q9T017; -.
DR STRING; 3702.AT4G11190.1; -.
DR PaxDb; Q9T017; -.
DR PRIDE; Q9T017; -.
DR ProteomicsDB; 224105; -.
DR DNASU; 826721; -.
DR EnsemblPlants; AT4G11190.1; AT4G11190.1; AT4G11190.
DR GeneID; 826721; -.
DR Gramene; AT4G11190.1; AT4G11190.1; AT4G11190.
DR KEGG; ath:AT4G11190; -.
DR Araport; AT4G11190; -.
DR TAIR; locus:2136128; AT4G11190.
DR eggNOG; ENOG502RXV9; Eukaryota.
DR HOGENOM; CLU_087111_0_0_1; -.
DR InParanoid; Q9T017; -.
DR OMA; YFHDIIF; -.
DR OrthoDB; 1498667at2759; -.
DR PhylomeDB; Q9T017; -.
DR PRO; PR:Q9T017; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q9T017; baseline and differential.
DR Genevisible; Q9T017; AT.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR Gene3D; 2.40.480.10; -; 1.
DR InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR InterPro; IPR004265; Dirigent.
DR PANTHER; PTHR46442; PTHR46442; 1.
DR Pfam; PF03018; Dirigent; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..184
FT /note="Dirigent protein 13"
FT /id="PRO_0000422844"
FT CARBOHYD 55
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 119
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 36..182
FT /evidence="ECO:0000250"
SQ SEQUENCE 184 AA; 20939 MW; 511314B348AD77A9 CRC64;
MANQIYIISL IFLSVLLYQS TTVLSFRQPF NLAKPCKRFV FYLHNVAYDG DNTDNATSAA
IVNPLGLGDF SFGKFVIMDN PVTMDQNMLS EQVARVQGFF FYHGKTKYDT WLSWSVVFNS
TQHKGALNIM GENAFMEPTR DLPVVGGTGD FVMTRGIATF MTDLVEGSKY FRVKMDIKLY
ECYY
