ID   DIR17_ARATH             Reviewed;         271 AA.
AC   F4J4N3; Q8GYI9; Q9M2N9;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   28-JUN-2011, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Dirigent protein 17;
DE            Short=AtDIR17;
GN   Name=DIR17; OrderedLocusNames=At3g58090; ORFNames=T10K17.300;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130713; DOI=10.1038/35048706;
RA   Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA   Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA   Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA   Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA   Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA   Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA   Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA   Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA   Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA   Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA   Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA   Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA   de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA   Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA   Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA   Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA   Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA   Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA   Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA   Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA   Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA   Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA   Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA   Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT   "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL   Nature 408:820-822(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   STRAIN=cv. Columbia;
RX   PubMed=11910074; DOI=10.1126/science.1071006;
RA   Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA   Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA   Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA   Shinagawa A., Shinozaki K.;
RT   "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL   Science 296:141-145(2002).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA   Ralph S.G., Jancsik S., Bohlmann J.;
RT   "Dirigent proteins in conifer defense II: Extended gene discovery,
RT   phylogeny, and constitutive and stress-induced gene expression in spruce
RT   (Picea spp.).";
RL   Phytochemistry 68:1975-1991(2007).
CC   -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC       radical-coupling reaction, yielding optically active lignans from two
CC       molecules of coniferyl alcohol in the biosynthesis of lignans,
CC       flavonolignans, and alkaloids and thus plays a central role in plant
CC       secondary metabolism. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=F4J4N3-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=F4J4N3-2; Sequence=VSP_047339;
CC   -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAB67637.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AL132977; CAB67637.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE79740.1; -; Genomic_DNA.
DR   EMBL; AK117592; BAC42249.1; -; mRNA.
DR   PIR; T46031; T46031.
DR   RefSeq; NP_191368.2; NM_115671.2. [F4J4N3-1]
DR   AlphaFoldDB; F4J4N3; -.
DR   SMR; F4J4N3; -.
DR   STRING; 3702.AT3G58090.1; -.
DR   PaxDb; F4J4N3; -.
DR   PRIDE; F4J4N3; -.
DR   ProteomicsDB; 222147; -. [F4J4N3-1]
DR   EnsemblPlants; AT3G58090.1; AT3G58090.1; AT3G58090. [F4J4N3-1]
DR   GeneID; 3769734; -.
DR   Gramene; AT3G58090.1; AT3G58090.1; AT3G58090. [F4J4N3-1]
DR   KEGG; ath:AT3G58090; -.
DR   Araport; AT3G58090; -.
DR   TAIR; locus:2095798; AT3G58090.
DR   eggNOG; ENOG502QUX4; Eukaryota.
DR   HOGENOM; CLU_1027969_0_0_1; -.
DR   InParanoid; F4J4N3; -.
DR   OMA; SADQNNF; -.
DR   OrthoDB; 1307715at2759; -.
DR   PRO; PR:F4J4N3; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; F4J4N3; baseline and differential.
DR   Genevisible; F4J4N3; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR   Gene3D; 2.40.480.10; -; 1.
DR   InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR   InterPro; IPR004265; Dirigent.
DR   PANTHER; PTHR21495; PTHR21495; 1.
DR   Pfam; PF03018; Dirigent; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Apoplast; Glycoprotein; Reference proteome; Secreted.
FT   CHAIN           1..271
FT                   /note="Dirigent protein 17"
FT                   /id="PRO_0000422848"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        255
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         1..165
FT                   /note="MEDTGSIKQEAQSHPPGIFEIPGEPAVVINGVPDEPQTDCMIAKDEPISSGT
FT                   VGSGEWLEGREVRKFFLGRYYSGTVTKFDKQSGWYRVEYEDGDSEDLDWSELEEVLLPL
FT                   DTKNSNTNAQSEYGEAGQRVNVKAPYPGHKPEKLVTTTVKAPYPGHKPEKLIPL -> M
FT                   VAV (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:11910074"
FT                   /id="VSP_047339"
SQ   SEQUENCE   271 AA;  29897 MW;  98FC1381DFC03BA0 CRC64;
     MEDTGSIKQE AQSHPPGIFE IPGEPAVVIN GVPDEPQTDC MIAKDEPISS GTVGSGEWLE
     GREVRKFFLG RYYSGTVTKF DKQSGWYRVE YEDGDSEDLD WSELEEVLLP LDTKNSNTNA
     QSEYGEAGQR VNVKAPYPGH KPEKLVTTTV KAPYPGHKPE KLIPLVDDIL TVGPEITSEE
     VGRAQGIFAS ADQNNFGLLM AFNFVFTKGE FSGSTVSMYG RNPIFSKVRE MPIIGGTGAF
     RFGRGYAQAK TFTFNTTSGN AVVKYNVYIW H