ID   DIR1_ARATH              Reviewed;         182 AA.
AC   Q9FIG6;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Dirigent protein 1;
DE            Short=AtDIR1;
DE   Flags: Precursor;
GN   Name=DIR1; OrderedLocusNames=At5g42510; ORFNames=MDH9.21;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA   Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT   features of the regions of 1,081,958 bp covered by seventeen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:379-391(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Bautista-Mercan V.R., Kim C.J., Chen H., Quan R., De Los Reyes C.,
RA   Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA   Ralph S.G., Jancsik S., Bohlmann J.;
RT   "Dirigent proteins in conifer defense II: Extended gene discovery,
RT   phylogeny, and constitutive and stress-induced gene expression in spruce
RT   (Picea spp.).";
RL   Phytochemistry 68:1975-1991(2007).
CC   -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC       radical-coupling reaction, yielding optically active lignans from two
CC       molecules of coniferyl alcohol in the biosynthesis of lignans,
CC       flavonolignans, and alkaloids and thus plays a central role in plant
CC       secondary metabolism. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC       {ECO:0000305}.
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DR   EMBL; AB016888; BAB10492.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED94819.1; -; Genomic_DNA.
DR   EMBL; BT030625; ABR46205.1; -; mRNA.
DR   RefSeq; NP_199066.1; NM_123616.2.
DR   AlphaFoldDB; Q9FIG6; -.
DR   SMR; Q9FIG6; -.
DR   STRING; 3702.AT5G42510.1; -.
DR   PaxDb; Q9FIG6; -.
DR   PRIDE; Q9FIG6; -.
DR   ProteomicsDB; 224120; -.
DR   EnsemblPlants; AT5G42510.1; AT5G42510.1; AT5G42510.
DR   GeneID; 834258; -.
DR   Gramene; AT5G42510.1; AT5G42510.1; AT5G42510.
DR   KEGG; ath:AT5G42510; -.
DR   Araport; AT5G42510; -.
DR   TAIR; locus:2162366; AT5G42510.
DR   eggNOG; ENOG502RXST; Eukaryota.
DR   HOGENOM; CLU_087111_2_0_1; -.
DR   InParanoid; Q9FIG6; -.
DR   OMA; HDKLYVM; -.
DR   OrthoDB; 1307715at2759; -.
DR   PhylomeDB; Q9FIG6; -.
DR   PRO; PR:Q9FIG6; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FIG6; baseline and differential.
DR   Genevisible; Q9FIG6; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR   Gene3D; 2.40.480.10; -; 1.
DR   InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR   InterPro; IPR004265; Dirigent.
DR   PANTHER; PTHR21495; PTHR21495; 1.
DR   Pfam; PF03018; Dirigent; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..182
FT                   /note="Dirigent protein 1"
FT                   /id="PRO_0000422832"
FT   CARBOHYD        125
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   182 AA;  20131 MW;  2A51B5B9067FD001 CRC64;
     MAKRFLLLLP LLSSILLLAV SVTAYSTTTP YQGYKPEKFT HLHFYFHDVI SGDKPTAVKV
     AEARPTTTLN VKFGVIMIAD DPLTEGPDPS SKEVGRAQGM YASTAMKDIV FTMVFNYVFT
     AGEFNGSTIA VYGRNDIFSK VRELPIIGGT GAFRFARGYA LPKTYKIVGL DAVVEYNVFI
     WH