DIR20_ARATH
ID DIR20_ARATH Reviewed; 187 AA.
AC Q9C891; Q8L7P2;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 104.
DE RecName: Full=Dirigent protein 20;
DE Short=AtDIR20;
DE Flags: Precursor;
GN Name=DIR20; OrderedLocusNames=At1g55210; ORFNames=F7A10.7;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA Ralph S.G., Jancsik S., Bohlmann J.;
RT "Dirigent proteins in conifer defense II: Extended gene discovery,
RT phylogeny, and constitutive and stress-induced gene expression in spruce
RT (Picea spp.).";
RL Phytochemistry 68:1975-1991(2007).
CC -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC radical-coupling reaction, yielding optically active lignans from two
CC molecules of coniferyl alcohol in the biosynthesis of lignans,
CC flavonolignans, and alkaloids and thus plays a central role in plant
CC secondary metabolism. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC {ECO:0000305}.
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DR EMBL; AC027034; AAG51557.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33205.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33206.1; -; Genomic_DNA.
DR EMBL; AY128336; AAM91539.1; -; mRNA.
DR EMBL; BT015679; AAU15178.1; -; mRNA.
DR PIR; H96593; H96593.
DR RefSeq; NP_001185232.1; NM_001198303.1.
DR RefSeq; NP_175917.1; NM_104394.4.
DR AlphaFoldDB; Q9C891; -.
DR SMR; Q9C891; -.
DR STRING; 3702.AT1G55210.1; -.
DR PaxDb; Q9C891; -.
DR PRIDE; Q9C891; -.
DR ProteomicsDB; 224121; -.
DR EnsemblPlants; AT1G55210.1; AT1G55210.1; AT1G55210.
DR EnsemblPlants; AT1G55210.2; AT1G55210.2; AT1G55210.
DR GeneID; 841964; -.
DR Gramene; AT1G55210.1; AT1G55210.1; AT1G55210.
DR Gramene; AT1G55210.2; AT1G55210.2; AT1G55210.
DR KEGG; ath:AT1G55210; -.
DR Araport; AT1G55210; -.
DR TAIR; locus:2035661; AT1G55210.
DR eggNOG; ENOG502RXST; Eukaryota.
DR HOGENOM; CLU_087111_2_1_1; -.
DR InParanoid; Q9C891; -.
DR OMA; YWQDILS; -.
DR OrthoDB; 1307715at2759; -.
DR PhylomeDB; Q9C891; -.
DR PRO; PR:Q9C891; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C891; baseline and differential.
DR Genevisible; Q9C891; AT.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR Gene3D; 2.40.480.10; -; 1.
DR InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR InterPro; IPR004265; Dirigent.
DR PANTHER; PTHR21495; PTHR21495; 1.
DR Pfam; PF03018; Dirigent; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..187
FT /note="Dirigent protein 20"
FT /id="PRO_0000422851"
FT CARBOHYD 71
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 92
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 127
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 69
FT /note="V -> F (in Ref. 3; AAM91539)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 187 AA; 20618 MW; 55C23D29553997A5 CRC64;
MAKLIFFLAV QILFLAVVSS AGDGEDFART MDRKLLGLHK KEKLTHFKVY WHDILSGPNP
TSIMIQPPVT NSSYFGAISM IDNALTAKVP MNSTVLGQAQ GFYAGAAQKE LGFLMAMNFA
FKTGKYNGST ITILGRNTAL SEVREMPIVG GSGLFRFARG YVEARTKWIN LKNGDATVEY
SCYVLHY
