ID   DIR23_ARATH             Reviewed;         187 AA.
AC   Q84TH6; Q9SKQ3;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Dirigent protein 23;
DE            Short=AtDIR23;
DE   Flags: Precursor;
GN   Name=DIR23; OrderedLocusNames=At2g21100; ORFNames=F26H11.14;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Cheuk R.F., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT   "Arabidopsis ORF clones.";
RL   Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA   Ralph S.G., Jancsik S., Bohlmann J.;
RT   "Dirigent proteins in conifer defense II: Extended gene discovery,
RT   phylogeny, and constitutive and stress-induced gene expression in spruce
RT   (Picea spp.).";
RL   Phytochemistry 68:1975-1991(2007).
CC   -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC       radical-coupling reaction, yielding optically active lignans from two
CC       molecules of coniferyl alcohol in the biosynthesis of lignans,
CC       flavonolignans, and alkaloids and thus plays a central role in plant
CC       secondary metabolism. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAD29806.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC006264; AAD29806.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; AEC07123.1; -; Genomic_DNA.
DR   EMBL; CP002685; ANM61894.1; -; Genomic_DNA.
DR   EMBL; BT005788; AAO64191.1; -; mRNA.
DR   EMBL; BT015116; AAT71988.1; -; mRNA.
DR   EMBL; AK228386; BAF00324.1; -; mRNA.
DR   PIR; B84597; B84597.
DR   RefSeq; NP_001324083.1; NM_001335731.1.
DR   RefSeq; NP_850009.1; NM_179678.2.
DR   AlphaFoldDB; Q84TH6; -.
DR   SMR; Q84TH6; -.
DR   STRING; 3702.AT2G21100.1; -.
DR   PaxDb; Q84TH6; -.
DR   PRIDE; Q84TH6; -.
DR   ProteomicsDB; 222216; -.
DR   EnsemblPlants; AT2G21100.1; AT2G21100.1; AT2G21100.
DR   EnsemblPlants; AT2G21100.2; AT2G21100.2; AT2G21100.
DR   GeneID; 816645; -.
DR   Gramene; AT2G21100.1; AT2G21100.1; AT2G21100.
DR   Gramene; AT2G21100.2; AT2G21100.2; AT2G21100.
DR   KEGG; ath:AT2G21100; -.
DR   Araport; AT2G21100; -.
DR   TAIR; locus:2047002; AT2G21100.
DR   eggNOG; ENOG502RY4Y; Eukaryota.
DR   HOGENOM; CLU_087111_2_1_1; -.
DR   InParanoid; Q84TH6; -.
DR   OMA; YAIAKTH; -.
DR   OrthoDB; 1307715at2759; -.
DR   PhylomeDB; Q84TH6; -.
DR   PRO; PR:Q84TH6; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q84TH6; baseline and differential.
DR   Genevisible; Q84TH6; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR   Gene3D; 2.40.480.10; -; 1.
DR   InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR   InterPro; IPR004265; Dirigent.
DR   PANTHER; PTHR21495; PTHR21495; 1.
DR   Pfam; PF03018; Dirigent; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..187
FT                   /note="Dirigent protein 23"
FT                   /id="PRO_0000422854"
FT   CARBOHYD        182
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   187 AA;  20594 MW;  DADE873A946B6C27 CRC64;
     MAKEEYVSRM LVMLIMIMPL VAQGSRLHSW ANRLEETGKD KVTNLQFYFH DTLSGKNPTA
     VKVAQGTDTE KSPTLFGAVF MVDDALTETA DPKSKLVGRA QGLYGSSCKE EVGLIMAMSF
     CFEDGPYKDS TISMIGKNSA MNPIREMPIV GGTGMFRMAR GYAIARTNWF DPKTGDAIVG
     YNVTIMH