ID   DIR25_ARATH             Reviewed;         389 AA.
AC   Q9LQQ0;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Dirigent protein 25;
DE            Short=AtDIR25;
DE   Flags: Precursor;
GN   Name=DIR25; OrderedLocusNames=At1g07730; ORFNames=F24B9.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA   Ralph S.G., Jancsik S., Bohlmann J.;
RT   "Dirigent proteins in conifer defense II: Extended gene discovery,
RT   phylogeny, and constitutive and stress-induced gene expression in spruce
RT   (Picea spp.).";
RL   Phytochemistry 68:1975-1991(2007).
CC   -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC       radical-coupling reaction, yielding optically active lignans from two
CC       molecules of coniferyl alcohol in the biosynthesis of lignans,
CC       flavonolignans, and alkaloids and thus plays a central role in plant
CC       secondary metabolism. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC       {ECO:0000305}.
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DR   EMBL; AC007583; AAF75080.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE28172.1; -; Genomic_DNA.
DR   PIR; F86212; F86212.
DR   RefSeq; NP_973782.1; NM_202053.1.
DR   AlphaFoldDB; Q9LQQ0; -.
DR   SMR; Q9LQQ0; -.
DR   STRING; 3702.AT1G07730.2; -.
DR   PaxDb; Q9LQQ0; -.
DR   ProteomicsDB; 222207; -.
DR   EnsemblPlants; AT1G07730.2; AT1G07730.2; AT1G07730.
DR   GeneID; 2745746; -.
DR   Gramene; AT1G07730.2; AT1G07730.2; AT1G07730.
DR   KEGG; ath:AT1G07730; -.
DR   Araport; AT1G07730; -.
DR   TAIR; locus:1006589878; AT1G07730.
DR   eggNOG; ENOG502SAD4; Eukaryota.
DR   HOGENOM; CLU_059816_0_0_1; -.
DR   InParanoid; Q9LQQ0; -.
DR   OMA; MAGCKVL; -.
DR   PhylomeDB; Q9LQQ0; -.
DR   PRO; PR:Q9LQQ0; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9LQQ0; baseline and differential.
DR   Genevisible; Q9LQQ0; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR   Gene3D; 2.40.480.10; -; 1.
DR   InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR   InterPro; IPR004265; Dirigent.
DR   PANTHER; PTHR46215; PTHR46215; 1.
DR   Pfam; PF03018; Dirigent; 1.
PE   3: Inferred from homology;
KW   Apoplast; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000255"
FT   CHAIN           22..389
FT                   /note="Dirigent protein 25"
FT                   /id="PRO_0000422856"
FT   REGION          50..135
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        58..99
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        115..131
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   389 AA;  38336 MW;  4585CFF779817312 CRC64;
     MAGCKVLFFL ILALAITFVS AARLLDEEED IGLVPLPTTS PGPLPTVGLG PFPTANSGPA
     TGIASGTGSA SGGLGSLGTN TGPGPLSTTG SSLLPVASSG TLPVTGPGPL PTSSGLLPGA
     SSGNLPGSGS GPLPTVGSGA AATGLGAGAG SVIGGSVPDN TLVFFMHDIL GGSNPTARAV
     TGVVANAALS GQIPFAKPNG ANLPVSNGVP SDNNNNGILN NNNVPLLVGL GGTTSNILQN
     NGNNMLNGLP VANGGQLPSG SSLQMLMFGT LTVMDNELTE GHELGSGLLG KAQGFYVASA
     LDGTSQTMAF TAMFESGGYE DSISFFGVHR TAASESHLGV MGGTGKYVNA RGFAIVKTFT
     GSSGTQQQQP HQFTDGLETV LECTVYLSY