ID   DIR2_ARATH              Reviewed;         185 AA.
AC   Q9FIG7;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Dirigent protein 2;
DE            Short=AtDIR2;
DE   Flags: Precursor;
GN   Name=DIR2; OrderedLocusNames=At5g42500; ORFNames=MDH9.20;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10048488; DOI=10.1093/dnares/5.6.379;
RA   Asamizu E., Sato S., Kaneko T., Nakamura Y., Kotani H., Miyajima N.,
RA   Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. VIII. Sequence
RT   features of the regions of 1,081,958 bp covered by seventeen physically
RT   assigned P1 and TAC clones.";
RL   DNA Res. 5:379-391(1998).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA   Ralph S.G., Jancsik S., Bohlmann J.;
RT   "Dirigent proteins in conifer defense II: Extended gene discovery,
RT   phylogeny, and constitutive and stress-induced gene expression in spruce
RT   (Picea spp.).";
RL   Phytochemistry 68:1975-1991(2007).
CC   -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC       radical-coupling reaction, yielding optically active lignans from two
CC       molecules of coniferyl alcohol in the biosynthesis of lignans,
CC       flavonolignans, and alkaloids and thus plays a central role in plant
CC       secondary metabolism. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC       {ECO:0000305}.
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DR   EMBL; AB016888; BAB10491.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED94817.1; -; Genomic_DNA.
DR   EMBL; AY093095; AAM13094.1; -; mRNA.
DR   EMBL; BT008442; AAP37801.1; -; mRNA.
DR   RefSeq; NP_199065.1; NM_123615.3.
DR   AlphaFoldDB; Q9FIG7; -.
DR   SMR; Q9FIG7; -.
DR   STRING; 3702.AT5G42500.1; -.
DR   PaxDb; Q9FIG7; -.
DR   PRIDE; Q9FIG7; -.
DR   ProteomicsDB; 224164; -.
DR   EnsemblPlants; AT5G42500.1; AT5G42500.1; AT5G42500.
DR   GeneID; 834257; -.
DR   Gramene; AT5G42500.1; AT5G42500.1; AT5G42500.
DR   KEGG; ath:AT5G42500; -.
DR   Araport; AT5G42500; -.
DR   TAIR; locus:2162361; AT5G42500.
DR   eggNOG; ENOG502RXST; Eukaryota.
DR   HOGENOM; CLU_087111_2_1_1; -.
DR   InParanoid; Q9FIG7; -.
DR   OMA; WASASME; -.
DR   OrthoDB; 1307715at2759; -.
DR   PhylomeDB; Q9FIG7; -.
DR   PRO; PR:Q9FIG7; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FIG7; baseline and differential.
DR   Genevisible; Q9FIG7; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR   Gene3D; 2.40.480.10; -; 1.
DR   InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR   InterPro; IPR004265; Dirigent.
DR   PANTHER; PTHR21495; PTHR21495; 1.
DR   Pfam; PF03018; Dirigent; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   CHAIN           28..185
FT                   /note="Dirigent protein 2"
FT                   /id="PRO_0000422833"
FT   CARBOHYD        70
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        111
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        128
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   185 AA;  20438 MW;  065B9F92334E4365 CRC64;
     MAKRFLLLLP LLSTILLLSV SVTESEAYST TKPCQGYKPD KFTHLHFYFH DVISGDKPTA
     VKVAEARRTN SSNVNFGVIM IADDPLTEGP DPSSKEVGRA QGMYALTAMK NISFTMVFNL
     AFTAGEFNGS TVAMYGRNEI FSKVREMPII GGTGAFRFAR GYAQAKTYKV VGLDAVVEYN
     VFIWH