DIR3_ARATH
ID DIR3_ARATH Reviewed; 191 AA.
AC Q9FI66;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Dirigent protein 3;
DE Short=AtDIR3;
DE Flags: Precursor;
GN Name=DIR3; OrderedLocusNames=At5g49040; ORFNames=K19E20.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10470850; DOI=10.1093/dnares/6.3.183;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Kotani H.,
RA Miyajima N., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IX. Sequence
RT features of the regions of 1,011,550 bp covered by seventeen P1 and TAC
RT clones.";
RL DNA Res. 6:183-195(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA Ralph S.G., Jancsik S., Bohlmann J.;
RT "Dirigent proteins in conifer defense II: Extended gene discovery,
RT phylogeny, and constitutive and stress-induced gene expression in spruce
RT (Picea spp.).";
RL Phytochemistry 68:1975-1991(2007).
CC -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC radical-coupling reaction, yielding optically active lignans from two
CC molecules of coniferyl alcohol in the biosynthesis of lignans,
CC flavonolignans, and alkaloids and thus plays a central role in plant
CC secondary metabolism. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC {ECO:0000305}.
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DR EMBL; AB017061; BAB10328.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95765.1; -; Genomic_DNA.
DR RefSeq; NP_199715.1; NM_124281.1.
DR AlphaFoldDB; Q9FI66; -.
DR SMR; Q9FI66; -.
DR STRING; 3702.AT5G49040.1; -.
DR PaxDb; Q9FI66; -.
DR PRIDE; Q9FI66; -.
DR ProteomicsDB; 222020; -.
DR EnsemblPlants; AT5G49040.1; AT5G49040.1; AT5G49040.
DR GeneID; 834963; -.
DR Gramene; AT5G49040.1; AT5G49040.1; AT5G49040.
DR KEGG; ath:AT5G49040; -.
DR Araport; AT5G49040; -.
DR TAIR; locus:2154359; AT5G49040.
DR eggNOG; ENOG502RXST; Eukaryota.
DR HOGENOM; CLU_087111_2_1_1; -.
DR InParanoid; Q9FI66; -.
DR OMA; AMTFAFK; -.
DR OrthoDB; 1307715at2759; -.
DR PhylomeDB; Q9FI66; -.
DR PRO; PR:Q9FI66; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FI66; baseline.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR Gene3D; 2.40.480.10; -; 1.
DR InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR InterPro; IPR004265; Dirigent.
DR PANTHER; PTHR21495; PTHR21495; 1.
DR Pfam; PF03018; Dirigent; 1.
PE 3: Inferred from homology;
KW Apoplast; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..191
FT /note="Dirigent protein 3"
FT /id="PRO_0000422834"
FT CARBOHYD 96
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 131
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 191 AA; 20897 MW; 6392FFD74DB83B3E CRC64;
MSKLILILTA QILLLTATAL AGKNGEDFAR TINRKHLGLG KKEKLTHLRV YWHDIVTGRN
PSSIRIQGPV AKYSSSSYFG SITMIDNALT LDVPINSTVV GQAQGMYVGA AQKEIGLLMA
MNLAFKTGKY NGSTITILGR NTVMSKVREM PVVGGSGMFR FARGYVEART KLFDMKTGDA
TVESNCYILH Y