ID   DIR4_ARATH              Reviewed;         186 AA.
AC   Q9SKQ2; A0MEN7;
DT   26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Dirigent protein 4;
DE            Short=AtDIR4;
DE   Flags: Precursor;
GN   Name=DIR4; OrderedLocusNames=At2g21110; ORFNames=F26H11.13;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA   Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT   "Simultaneous high-throughput recombinational cloning of open reading
RT   frames in closed and open configurations.";
RL   Plant Biotechnol. J. 4:317-324(2006).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA   Ralph S.G., Jancsik S., Bohlmann J.;
RT   "Dirigent proteins in conifer defense II: Extended gene discovery,
RT   phylogeny, and constitutive and stress-induced gene expression in spruce
RT   (Picea spp.).";
RL   Phytochemistry 68:1975-1991(2007).
CC   -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC       radical-coupling reaction, yielding optically active lignans from two
CC       molecules of coniferyl alcohol in the biosynthesis of lignans,
CC       flavonolignans, and alkaloids and thus plays a central role in plant
CC       secondary metabolism. {ECO:0000250}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABK28504.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AC006264; AAD29805.1; -; Genomic_DNA.
DR   EMBL; CP002685; AEC07124.1; -; Genomic_DNA.
DR   EMBL; DQ446539; ABE65841.1; -; mRNA.
DR   EMBL; DQ653009; ABK28504.1; ALT_SEQ; mRNA.
DR   PIR; C84597; C84597.
DR   RefSeq; NP_179707.1; NM_127681.2.
DR   AlphaFoldDB; Q9SKQ2; -.
DR   SMR; Q9SKQ2; -.
DR   STRING; 3702.AT2G21110.1; -.
DR   PaxDb; Q9SKQ2; -.
DR   PRIDE; Q9SKQ2; -.
DR   EnsemblPlants; AT2G21110.1; AT2G21110.1; AT2G21110.
DR   GeneID; 816646; -.
DR   Gramene; AT2G21110.1; AT2G21110.1; AT2G21110.
DR   KEGG; ath:AT2G21110; -.
DR   Araport; AT2G21110; -.
DR   TAIR; locus:2047127; AT2G21110.
DR   eggNOG; ENOG502RXRA; Eukaryota.
DR   HOGENOM; CLU_087111_2_1_1; -.
DR   InParanoid; Q9SKQ2; -.
DR   OMA; AHGQYYS; -.
DR   OrthoDB; 1409732at2759; -.
DR   PhylomeDB; Q9SKQ2; -.
DR   PRO; PR:Q9SKQ2; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q9SKQ2; baseline and differential.
DR   Genevisible; Q9SKQ2; AT.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009699; P:phenylpropanoid biosynthetic process; IEA:UniProt.
DR   Gene3D; 2.40.480.10; -; 1.
DR   InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR   InterPro; IPR004265; Dirigent.
DR   PANTHER; PTHR21495; PTHR21495; 1.
DR   Pfam; PF03018; Dirigent; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Glycoprotein; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..186
FT                   /note="Dirigent protein 4"
FT                   /id="PRO_0000422835"
FT   CARBOHYD        67
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        126
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        169
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        180
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   186 AA;  20427 MW;  6EEA3FF1AE39BC22 CRC64;
     MGKNLGLVVS FYLCITFALG EYFSETRPIT PKQLVVTNLH FFFHDTLTAP NPSAILIAKP
     THTRGDNDSS PSPFGSLFAL DDPLTVGPDP KSEKIGNARG MYVSSGKHVP TLTMYVDFGF
     TSGKFNGSSI AVFSRNTITE KEREVAVVGG RGRFRMARGV AQLNTYYVNL TNGDAIVEYN
     VTLYHY