DIR5_ARATH
ID DIR5_ARATH Reviewed; 182 AA.
AC Q9SH66;
DT 26-JUN-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 112.
DE RecName: Full=Dirigent protein 5;
DE Short=AtDIR5;
DE Flags: Precursor;
GN Name=DIR5; OrderedLocusNames=At1g64160; ORFNames=F22C12.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=17590394; DOI=10.1016/j.phytochem.2007.04.042;
RA Ralph S.G., Jancsik S., Bohlmann J.;
RT "Dirigent proteins in conifer defense II: Extended gene discovery,
RT phylogeny, and constitutive and stress-induced gene expression in spruce
RT (Picea spp.).";
RL Phytochemistry 68:1975-1991(2007).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND GENE FAMILY.
RC STRAIN=cv. Columbia;
RX PubMed=22854967; DOI=10.1074/jbc.m112.387423;
RA Kim K.-W., Moinuddin S.G.A., Atwell K.M., Costa M.A., Davin L.B.,
RA Lewis N.G.;
RT "Opposite stereoselectivities of dirigent proteins in Arabidopsis and
RT schizandra species.";
RL J. Biol. Chem. 287:33957-33972(2012).
CC -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC radical-coupling reaction, yielding optically active lignans from two
CC molecules of coniferyl alcohol in the biosynthesis of lignans,
CC flavonolignans, and alkaloids and thus plays a central role in plant
CC secondary metabolism. Enantiocomplementary dirigent protein that
CC mediates the laccase-catalyzed enantioselective oxidative phenol
CC coupling of (E)-coniferyl alcohol to (-)-pinoresinol.
CC {ECO:0000269|PubMed:22854967}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Confined to shoot meristem, vascular region of
CC cotyledons and siliques abscission zone. {ECO:0000269|PubMed:22854967}.
CC -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC {ECO:0000305}.
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DR EMBL; AC007764; AAF24580.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34203.1; -; Genomic_DNA.
DR EMBL; BT010486; AAQ65109.1; -; mRNA.
DR EMBL; AK175255; BAD43018.1; -; mRNA.
DR EMBL; AK176738; BAD44501.1; -; mRNA.
DR PIR; A96666; A96666.
DR RefSeq; NP_176598.1; NM_105089.3.
DR AlphaFoldDB; Q9SH66; -.
DR SMR; Q9SH66; -.
DR STRING; 3702.AT1G64160.1; -.
DR iPTMnet; Q9SH66; -.
DR PaxDb; Q9SH66; -.
DR PRIDE; Q9SH66; -.
DR ProteomicsDB; 222218; -.
DR EnsemblPlants; AT1G64160.1; AT1G64160.1; AT1G64160.
DR GeneID; 842720; -.
DR Gramene; AT1G64160.1; AT1G64160.1; AT1G64160.
DR KEGG; ath:AT1G64160; -.
DR Araport; AT1G64160; -.
DR TAIR; locus:2024593; AT1G64160.
DR eggNOG; ENOG502RXV9; Eukaryota.
DR HOGENOM; CLU_087111_0_0_1; -.
DR InParanoid; Q9SH66; -.
DR OMA; IFESDTM; -.
DR OrthoDB; 1498667at2759; -.
DR PhylomeDB; Q9SH66; -.
DR PRO; PR:Q9SH66; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SH66; baseline and differential.
DR Genevisible; Q9SH66; AT.
DR GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR GO; GO:0042349; F:guiding stereospecific synthesis activity; IDA:TAIR.
DR GO; GO:1901599; P:(-)-pinoresinol biosynthetic process; IDA:TAIR.
DR Gene3D; 2.40.480.10; -; 1.
DR InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR InterPro; IPR004265; Dirigent.
DR PANTHER; PTHR46442; PTHR46442; 1.
DR Pfam; PF03018; Dirigent; 1.
PE 2: Evidence at transcript level;
KW Apoplast; Disulfide bond; Glycoprotein; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000250"
FT CHAIN 24..182
FT /note="Dirigent protein 5"
FT /id="PRO_0000422836"
FT CARBOHYD 54
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 118
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 35..181
FT /evidence="ECO:0000250"
SQ SEQUENCE 182 AA; 20726 MW; 6000E7975507F09E CRC64;
MVGQMKSFLF LFVFLVLTKT VISARKPSKS QPKPCKNFVL YYHDIMFGVD DVQNATSAAV
TNPPGLGNFK FGKLVIFDDP MTIDKNFQSE PVARAQGFYF YDMKNDYNAW FAYTLVFNST
QHKGTLNIMG ADLMMVQSRD LSVVGGTGDF FMSRGIVTFE TDTFEGAKYF RVKMDIKLYE
CY
