ID   DIR_SINHE               Reviewed;         193 AA.
AC   Q1ZZU9;
DT   10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT   18-APR-2006, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Dirigent protein {ECO:0000303|Ref.1};
DE            Short=PhDPO {ECO:0000303|PubMed:23653238};
DE   Flags: Precursor;
GN   Name=DIR {ECO:0000303|PubMed:26359402};
GN   Synonyms=DPO {ECO:0000303|PubMed:23653238, ECO:0000303|Ref.1},
GN   Phex2312 {ECO:0000303|PubMed:26359402};
OS   Sinopodophyllum hexandrum (Himalayan may apple) (Podophyllum hexandrum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Ranunculales; Berberidaceae; Podophylloideae;
OC   Sinopodophyllum.
OX   NCBI_TaxID=93608;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Lan X.;
RT   "A dirigent protein oxidase gene from Tibet Podophyllum.";
RL   Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   TISSUE SPECIFICITY, AND INDUCTION BY WOUNDING; UV-LIGHT AND JASMONIC ACID.
RX   PubMed=23653238; DOI=10.1007/s00709-013-0505-z;
RA   Wankhede D.P., Biswas D.K., Rajkumar S., Sinha A.K.;
RT   "Expressed sequence tags and molecular cloning and characterization of gene
RT   encoding pinoresinol/lariciresinol reductase from Podophyllum hexandrum.";
RL   Protoplasma 250:1239-1249(2013).
RN   [3]
RP   FUNCTION, BIOTECHNOLOGY, INDUCTION BY WOUNDING, TISSUE SPECIFICITY, AND
RP   PATHWAY.
RX   PubMed=26359402; DOI=10.1126/science.aac7202;
RA   Lau W., Sattely E.S.;
RT   "Six enzymes from mayapple that complete the biosynthetic pathway to the
RT   etoposide aglycone.";
RL   Science 349:1224-1228(2015).
CC   -!- FUNCTION: Dirigent proteins impart stereoselectivity on the phenoxy
CC       radical-coupling reaction, yielding optically active lignans from two
CC       molecules of coniferyl alcohol in the biosynthesis of lignans,
CC       flavonolignans, and alkaloids and thus plays a central role in plant
CC       secondary metabolism (By similarity). Also involved in the biosynthesis
CC       of etoposide, a chemotherapeutic compound of the topoisomerase
CC       inhibitor family (PubMed:26359402). {ECO:0000250|UniProtKB:Q9SUQ8,
CC       ECO:0000269|PubMed:26359402}.
CC   -!- PATHWAY: Aromatic compound metabolism; phenylpropanoid biosynthesis.
CC       {ECO:0000269|PubMed:26359402}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:Q9SUQ8}.
CC   -!- SUBCELLULAR LOCATION: Secreted, extracellular space, apoplast
CC       {ECO:0000250|UniProtKB:Q9SUQ8}.
CC   -!- TISSUE SPECIFICITY: Expressed in rhizomes, stems, and leaves.
CC       {ECO:0000269|PubMed:23653238, ECO:0000269|PubMed:26359402}.
CC   -!- INDUCTION: Transiently induced after wounding and by jasmonic acid
CC       (MeJA) (PubMed:26359402, PubMed:23653238). After an exposition to UV-
CC       light, first transiently induced before fading out (PubMed:23653238).
CC       {ECO:0000269|PubMed:23653238, ECO:0000269|PubMed:26359402}.
CC   -!- BIOTECHNOLOGY: Combinatorially expression of Sinopodophyllum hexandrum
CC       (mayapple) genes of the podophyllotoxin pathway (e.g. DIR, PLR, SDH,
CC       CYP719A23, OMT3, CYP71CU1, OMT1, 2-ODD, CYP71BE54 and CYP82D61) in
CC       Nicotiana benthamiana (tobacco) results in the production of the
CC       chemotherapeutic compound etoposide. {ECO:0000305|PubMed:26359402}.
CC   -!- SIMILARITY: Belongs to the plant dirigent protein family.
CC       {ECO:0000305}.
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DR   EMBL; DQ414685; ABD66756.1; -; mRNA.
DR   AlphaFoldDB; Q1ZZU9; -.
DR   SMR; Q1ZZU9; -.
DR   UniPathway; UPA00711; -.
DR   GO; GO:0048046; C:apoplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0102910; F:dirigent protein activity; IDA:UniProtKB.
DR   GO; GO:0019438; P:aromatic compound biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0009699; P:phenylpropanoid biosynthetic process; IDA:UniProtKB.
DR   GO; GO:0009753; P:response to jasmonic acid; IEP:UniProtKB.
DR   GO; GO:0009411; P:response to UV; IEP:UniProtKB.
DR   GO; GO:0009611; P:response to wounding; IEP:UniProtKB.
DR   Gene3D; 2.40.480.10; -; 1.
DR   InterPro; IPR044859; Allene_oxi_cyc_Dirigent.
DR   InterPro; IPR004265; Dirigent.
DR   PANTHER; PTHR46442; PTHR46442; 1.
DR   Pfam; PF03018; Dirigent; 1.
PE   2: Evidence at transcript level;
KW   Apoplast; Disulfide bond; Glycoprotein; Secreted; Signal.
FT   SIGNAL          1..29
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..193
FT                   /note="Dirigent protein"
FT                   /id="PRO_5008193436"
FT   CARBOHYD        59
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        129
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   DISULFID        40..192
FT                   /evidence="ECO:0000250|UniProtKB:Q9SUQ8"
SQ   SEQUENCE   193 AA;  21521 MW;  ACD882FDE0D3A87E CRC64;
     MGGEKAFSFI FLLFLCFFLA NLSASSAHPP RQKLKQRIPC KQLVLYFHDV VYNGHNKANA
     TASIVGAPQG ADLVKLAGEN HFGNVVVFDD PITLDNNFHS PPVGRAQGLY VYDKKDTFHS
     WLSFSFTLNT TMHQGTLIFM GADPILIKNR DITVVGGTGD FFMARGIATI ATDSYEGEVY
     FRLKVDIKLY ECW