DISC_DROME
ID DISC_DROME Reviewed; 568 AA.
AC P23792; Q53YG0; Q9VXJ4;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Protein disconnected;
GN Name=disco; ORFNames=CG9908;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND MUTAGENESIS OF CYS-94 AND CYS-127.
RC STRAIN=Canton-S;
RX PubMed=1901262; DOI=10.1002/j.1460-2075.1991.tb08013.x;
RA Heilig J.S., Freeman M., Laverty T., Lee K.J., Campos A.R., Rubin G.M.,
RA Steller H.;
RT "Isolation and characterization of the disconnected gene of Drosophila
RT melanogaster.";
RL EMBO J. 10:809-815(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP DEVELOPMENTAL STAGE.
RX PubMed=1901263; DOI=10.1002/j.1460-2075.1991.tb08014.x;
RA Lee K.J., Freeman M., Steller H.;
RT "Expression of the disconnected gene during development of Drosophila
RT melanogaster.";
RL EMBO J. 10:817-826(1991).
CC -!- FUNCTION: Required for the establishment of stable connections between
CC the larval optic nerves, the Bolwig's nerves, and their target cells in
CC the brain during embryonic development. {ECO:0000269|PubMed:1901262}.
CC -!- INTERACTION:
CC P23792; B6VQA1: dimm; NbExp=3; IntAct=EBI-152064, EBI-166061;
CC P23792; Q9TW27: DIP1; NbExp=2; IntAct=EBI-152064, EBI-443968;
CC P23792; Q8T3W6: hale; NbExp=4; IntAct=EBI-152064, EBI-163796;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in the adult head and very
CC low, but detectable, levels in the body. {ECO:0000269|PubMed:1901262}.
CC -!- DEVELOPMENTAL STAGE: Expression is seen as early as 6 hours of
CC embryonic development and continues throughout embryonic and larval
CC stages, increasing in abundance at each larval molt. The level remains
CC high during pupal life but decreases to low levels in the adult.
CC {ECO:0000269|PubMed:1901262, ECO:0000269|PubMed:1901263}.
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DR EMBL; X56232; CAA39689.1; -; Genomic_DNA.
DR EMBL; AE014298; AAF48568.1; -; Genomic_DNA.
DR EMBL; AE014298; ABC67185.1; -; Genomic_DNA.
DR EMBL; BT003295; AAO25055.1; -; mRNA.
DR PIR; S15008; S15008.
DR RefSeq; NP_001033847.1; NM_001038758.2.
DR RefSeq; NP_523362.2; NM_078638.4.
DR AlphaFoldDB; P23792; -.
DR BioGRID; 58921; 26.
DR DIP; DIP-19064N; -.
DR IntAct; P23792; 57.
DR STRING; 7227.FBpp0074028; -.
DR PaxDb; P23792; -.
DR DNASU; 32579; -.
DR EnsemblMetazoa; FBtr0074250; FBpp0074028; FBgn0000459.
DR EnsemblMetazoa; FBtr0100384; FBpp0099796; FBgn0000459.
DR GeneID; 32579; -.
DR KEGG; dme:Dmel_CG9908; -.
DR UCSC; CG9908-RA; d. melanogaster.
DR CTD; 32579; -.
DR FlyBase; FBgn0000459; disco.
DR VEuPathDB; VectorBase:FBgn0000459; -.
DR eggNOG; ENOG502QR8N; Eukaryota.
DR GeneTree; ENSGT00390000005844; -.
DR HOGENOM; CLU_521012_0_0_1; -.
DR InParanoid; P23792; -.
DR OMA; PPMWSLL; -.
DR OrthoDB; 96985at2759; -.
DR PhylomeDB; P23792; -.
DR SignaLink; P23792; -.
DR BioGRID-ORCS; 32579; 0 hits in 3 CRISPR screens.
DR ChiTaRS; disco; fly.
DR GenomeRNAi; 32579; -.
DR PRO; PR:P23792; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0000459; Expressed in mouthpart and 56 other tissues.
DR Genevisible; P23792; DM.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0003677; F:DNA binding; IDA:FlyBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:FlyBase.
DR GO; GO:0007469; P:antennal development; IMP:FlyBase.
DR GO; GO:0007420; P:brain development; IMP:FlyBase.
DR GO; GO:0007623; P:circadian rhythm; NAS:FlyBase.
DR GO; GO:0008062; P:eclosion rhythm; IMP:FlyBase.
DR GO; GO:0007479; P:leg disc proximal/distal pattern formation; IMP:FlyBase.
DR GO; GO:0045475; P:locomotor rhythm; IMP:FlyBase.
DR GO; GO:0045494; P:photoreceptor cell maintenance; NAS:FlyBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:FlyBase.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:FlyBase.
DR GO; GO:0010468; P:regulation of gene expression; IMP:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0007601; P:visual perception; IEA:UniProtKB-KW.
DR InterPro; IPR040436; Disconnected-like.
DR InterPro; IPR013087; Znf_C2H2_type.
DR PANTHER; PTHR15021; PTHR15021; 1.
DR SMART; SM00355; ZnF_C2H2; 2.
DR PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR PROSITE; PS50157; ZINC_FINGER_C2H2_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Repeat; Sensory transduction; Vision; Zinc;
KW Zinc-finger.
FT CHAIN 1..568
FT /note="Protein disconnected"
FT /id="PRO_0000046919"
FT ZN_FING 92..115
FT /note="C2H2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT ZN_FING 120..145
FT /note="C2H2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT REGION 16..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 134..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 220..364
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 391..419
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 501..568
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 28..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 233..247
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 263..279
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 297..364
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 391..413
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 524..551
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 552..568
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 94
FT /note="C->Y: In Disco-1."
FT /evidence="ECO:0000269|PubMed:1901262"
FT MUTAGEN 127
FT /note="C->S: In Disco-1656."
FT /evidence="ECO:0000269|PubMed:1901262"
FT CONFLICT 412
FT /note="D -> E (in Ref. 1; CAA39689)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 568 AA; 62369 MW; C875E72A2C2E644B CRC64;
MEHIMNPFMS PAYLLGHGPH SHQHVHSHLP SHPQPNAASP ASSPGGSSGS GSGSAAGSGT
GSGSSLKPRR WGSPPINLAG QFINPATGKK RVQCSICFKT FCDKGALKIH FSAVHLREMH
KCTVEGCNMV FSSRRSRNRH SANPNPKLHS PHIRRKISPH DGRTAQQFPV FSPGTAAAAA
AVAGRLPVAF PGLLPPPPPH HGHHPYVMFG GQAGLHGLGL LSTGCQDPDS GSVDNEQDAD
PEDDNDFVYV DMQANSSSPA ASSEDQEEHE RDNEQDEEMH CSLSLASSSS IAADEERAAD
QPLDFSLHKR RKSEQDREQE QEQEQERERE AEKEQEQDVE SDKEHEPEQE HELEREKRSP
SDAFSMDQLL GKRKRHDSTA SSSACSTAAA SSASSSSASA SANPPQTSIK MDLDPDSDSA
YMTSRRQMLP LPVLDLEEHH HLRLLQTQMF AAAAAAAATS QAPPTAFLPA GSPVDLAKDS
PPMWSLLSEM YRSMLLKTQH QQYNHHHQLQ QQHQQEQHHH LTLSHHHQEQ HHHLGHHHMG
HHHHHHHQHH QQQPQQQSPA ATNAPISV
