DIT22_ARATH
ID DIT22_ARATH Reviewed; 549 AA.
AC Q9FMF8;
DT 03-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Dicarboxylate transporter 2.2, chloroplastic;
DE AltName: Full=AtpDCT2;
DE Flags: Precursor;
GN Name=DIT2-2; OrderedLocusNames=At5g64280; ORFNames=MSJ1.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=12154133; DOI=10.1093/pcp/pcf109;
RA Taniguchi M., Taniguchi Y., Kawasaki M., Takeda S., Kato T., Sato S.,
RA Tabata S., Miyake H., Sugiyama T.;
RT "Identifying and characterizing plastidic 2-oxoglutarate/malate and
RT dicarboxylate transporters in Arabidopsis thaliana.";
RL Plant Cell Physiol. 43:706-717(2002).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=12887583; DOI=10.1046/j.1365-313x.2003.01806.x;
RA Renne P., Dressen U., Hebbeker U., Hille D., Flugge U.I., Westhoff P.,
RA Weber A.P.;
RT "The Arabidopsis mutant dct is deficient in the plastidic glutamate/malate
RT translocator DiT2.";
RL Plant J. 35:316-331(2003).
CC -!- FUNCTION: May be involved in the transport of dicarboxylate compounds.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000305}; Multi-pass membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, rosette and cauline leaves,
CC stems, flowers and siliques. {ECO:0000269|PubMed:12154133,
CC ECO:0000269|PubMed:12887583}.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC DIT1 subfamily. {ECO:0000305}.
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DR EMBL; AB008268; BAB09859.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97865.1; -; Genomic_DNA.
DR EMBL; AY045893; AAK76567.1; -; mRNA.
DR EMBL; AY133825; AAM91759.1; -; mRNA.
DR RefSeq; NP_201233.1; NM_125824.4.
DR AlphaFoldDB; Q9FMF8; -.
DR SMR; Q9FMF8; -.
DR BioGRID; 21791; 1.
DR IntAct; Q9FMF8; 1.
DR STRING; 3702.AT5G64280.1; -.
DR iPTMnet; Q9FMF8; -.
DR PaxDb; Q9FMF8; -.
DR PRIDE; Q9FMF8; -.
DR ProteomicsDB; 224166; -.
DR EnsemblPlants; AT5G64280.1; AT5G64280.1; AT5G64280.
DR GeneID; 836549; -.
DR Gramene; AT5G64280.1; AT5G64280.1; AT5G64280.
DR KEGG; ath:AT5G64280; -.
DR Araport; AT5G64280; -.
DR TAIR; locus:2173343; AT5G64280.
DR eggNOG; ENOG502QQ8W; Eukaryota.
DR HOGENOM; CLU_005170_7_3_1; -.
DR InParanoid; Q9FMF8; -.
DR OMA; RGEWLMA; -.
DR OrthoDB; 842017at2759; -.
DR PhylomeDB; Q9FMF8; -.
DR PRO; PR:Q9FMF8; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FMF8; baseline and differential.
DR Genevisible; Q9FMF8; AT.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0015140; F:malate transmembrane transporter activity; IEA:UniProt.
DR InterPro; IPR030676; CitT-rel.
DR InterPro; IPR001898; SLC13A/DASS.
DR PANTHER; PTHR42826; PTHR42826; 1.
DR Pfam; PF00939; Na_sulph_symp; 1.
DR TIGRFAMs; TIGR00785; dass; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; Membrane; Plastid; Plastid inner membrane; Reference proteome;
KW Transit peptide; Transmembrane; Transmembrane helix; Transport.
FT TRANSIT 1..54
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 55..549
FT /note="Dicarboxylate transporter 2.2, chloroplastic"
FT /id="PRO_0000419185"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 220..240
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 247..267
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 294..314
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 344..364
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 365..385
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 403..423
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 436..456
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 523..543
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 57..79
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..79
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 549 AA; 58747 MW; 61FD036CDB0D45F4 CRC64;
MESLALRSIS LSASYLSLHR SSSKSFALLP PSISVHTSPT LRSLSISSPR FTLRATASSL
PEEQNKPQPP PPSPPQPQGA KLIPLAISVS IGLIVRFLIP RPEQVTSQGW QLLSIFLFTI
SGLVLGPLPV GAWAFIGLTA SIVTKTLPFS TAFAAFTNEL IWLIAISFFF ARGFIKTGLG
DRIATYFVKW LGKSTLGLSY GLAFCETLMG LIMPSTMARA GGVFLPVIKS LAISAGSYPG
DPSSRKLGSF LIQTQLQCSG ASGAILLTSA AQNLLCLKLA REVGVVISNP WITWFKVASV
PAFVSLLCTP LIIYKLYPPE LKHTPEAPAA AAKKLERLGP ITKNEWIMLG AMAFTVSLWV
FGEAIGIASV VSAMIGLSTL LLLGVINWDD CLSDKSAWDS LTWFAVLIGM AGQLTNLGVV
AWMSDCVAKL LQSLSLTWPA SFIILQACYL LIHYLFASQT GHAGALYPPF LAMQIAAGVP
GVLAALCLAF NNNLSGALAH YSGGPAALYY GAGYVDLRDM FRVGFVMALV QAIIWGGVGS
FWWKFLGLY
