ID   DIT9_BPNF               Reviewed;         598 AA.
AC   Q04395; B7SSN0;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   05-OCT-2016, sequence version 2.
DT   29-SEP-2021, entry version 43.
DE   RecName: Full=Tail knob protein gp9 {ECO:0000250|UniProtKB:P04331};
DE   AltName: Full=Distal tube protein {ECO:0000250|UniProtKB:P04331};
DE   AltName: Full=Gene product 9;
DE            Short=gp9;
DE   AltName: Full=Protein p9 {ECO:0000250|UniProtKB:P04331};
DE   AltName: Full=Tail protein;
GN   Name=9;
OS   Bacillus phage Nf (Bacteriophage Nf).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Salasmaviridae; Picovirinae; Beecentumtrevirus.
OX   NCBI_TaxID=10753;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Yoo S.K., Braithwaite D.K., Ito J.;
RT   "Head fiber protein gene of bacteriophage Nf.";
RL   J. Gen. Appl. Microbiol. 36:365-368(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=19207565; DOI=10.1111/j.1462-2920.2008.01845.x;
RA   Castilla-Llorente V., Salas M., Meijer W.J.;
RT   "Different responses to Spo0A-mediated suppression of the related Bacillus
RT   subtilis phages Nf and phi29.";
RL   Environ. Microbiol. 11:1137-1149(2009).
CC   -!- FUNCTION: Distal (knob) tail protein that plugs the end of the tube
CC       before DNA ejection and forms a channel perforating the host membrane
CC       during ejection. {ECO:0000250|UniProtKB:P04331}.
CC   -!- SUBUNIT: Homohexamer; forms a hexameric tube structure with six
CC       flexible hydrophobic loops. {ECO:0000250|UniProtKB:P04331}.
CC   -!- SUBCELLULAR LOCATION: Virion {ECO:0000250|UniProtKB:P04331}.
CC       Note=Present in 6 copies in the virion. Located at the tip of the tail
CC       and constitutes most of the tail knob. {ECO:0000250|UniProtKB:P04331}.
CC   -!- SIMILARITY: Belongs to the picovirinae distal tube protein family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA36996.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X52807; CAA36996.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; EU622808; ACH57077.1; -; Genomic_DNA.
DR   PIR; S11639; S11639.
DR   SMR; Q04395; -.
DR   Proteomes; UP000000744; Genome.
DR   GO; GO:0098026; C:virus tail, tube; IEA:UniProtKB-KW.
DR   GO; GO:0044694; P:pore-mediated entry of viral genome into host cell; IEA:UniProtKB-KW.
DR   GO; GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism; IEA:UniProtKB-KW.
DR   InterPro; IPR031772; Caud_tail_N.
DR   Pfam; PF16838; Caud_tail_N; 1.
PE   3: Inferred from homology;
KW   Late protein; Pore-mediated penetration of viral genome into host cell;
KW   Reference proteome; Viral genome ejection through host cell envelope;
KW   Viral penetration into host cytoplasm; Viral short tail ejection system;
KW   Viral tail protein; Viral tail tube protein; Virion;
KW   Virus entry into host cell.
FT   CHAIN           1..598
FT                   /note="Tail knob protein gp9"
FT                   /id="PRO_0000106582"
SQ   SEQUENCE   598 AA;  67361 MW;  7B7BE4EC43F8755E CRC64;
     MAYVPLSGSD VRIFSDVPFS NDYKNTRWFT SADAQYSYFN AKPRVHVINE CNFVGLKEGT
     PHIKVNKRID DLYNACYMIF RNTQYSNKWF YCFVTRLEYV NSGVTNLYFE IDVIQTWMFD
     FKFQPSYIVR EHQTMWDANK EPLTNTIDEG LNYGSEYDIV AVEQYRPYGD LMFMVCISKS
     KMHATAGETF KAGEIAANIN GAPQPLSYYV HPFYEDGSSP KVTIGSNEVQ VAKPTDFLKN
     MFTQEHAVKN IVSLYVTDYI GLNIHYDESA KTMSLKDSMF EHTQISDDKH PNVNTIYLKE
     IKEYEEKTID TGYKFASFAN NEQSKLLMYP YCVTTITDFK GNQIDIKNEY VNGSNLKIQV
     RGSLGVSNKV TYGVKDYNAD STLSGSQKLT ASLQHSLINN NPNDVAIIND YLSAYLQGNK
     NSLENQKDSI LFNGVMGMLG NGIGAAASAA TGSAVGVASS ATGMVSSAGN AVLQIQGMQA
     KQADIANTPP QLVKMGGNTA YDYGNGYRGV YVIKKQIKEE YRNILSDFFK KYGYKTNLVK
     MPNLRTRKSY NYVQTKDCNI TGNLNNEDLQ KIRTIFDSGI TLWHADPVGD YTLNNEVI