DIT9_BPPH2
ID DIT9_BPPH2 Reviewed; 599 AA.
AC P04331; B3VMP5;
DT 20-MAR-1987, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 2.
DT 29-SEP-2021, entry version 56.
DE RecName: Full=Tail knob protein gp9 {ECO:0000303|PubMed:27309813};
DE AltName: Full=Distal tube protein {ECO:0000305};
DE AltName: Full=Gene product 9 {ECO:0000305};
DE Short=gp9 {ECO:0000305};
DE AltName: Full=Protein p9 {ECO:0000305};
GN Name=9 {ECO:0000312|EMBL:ACE96032.1};
OS Bacillus phage phi29 (Bacteriophage phi-29).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Salasmaviridae; Picovirinae; Salasvirus.
OX NCBI_TaxID=10756;
OH NCBI_TaxID=1423; Bacillus subtilis.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3803926; DOI=10.1016/0378-1119(86)90406-3;
RA Vlcek C., Paces V.;
RT "Nucleotide sequence of the late region of Bacillus phage phi 29 completes
RT the 19,285-bp sequence of phi 29 genome. Comparison with the homologous
RT sequence of phage PZA.";
RL Gene 46:215-225(1986).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Villegas A.P., Lingohr E.J., Ceyssens P.-J., Kropinski A.M.;
RL Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 265-599.
RX PubMed=6096227; DOI=10.1016/0378-1119(84)90108-2;
RA Garcia J.A., Mendez E., Salas M.;
RT "Cloning, nucleotide sequence and high level expression of the gene coding
RT for the connector protein of Bacillus subtilis phage phi 29.";
RL Gene 30:87-98(1984).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=8985375; DOI=10.1128/jvi.71.1.487-494.1997;
RA Trottier M., Guo P.;
RT "Approaches to determine stoichiometry of viral assembly components.";
RL J. Virol. 71:487-494(1997).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=11562162; DOI=10.1006/jsbi.2001.4375;
RA Peterson C., Simon M., Hodges J., Mertens P., Higgins L., Egelman E.,
RA Anderson D.;
RT "Composition and mass of the bacteriophage phi29 prohead and virion.";
RL J. Struct. Biol. 135:18-25(2001).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=18606992; DOI=10.1073/pnas.0803787105;
RA Xiang Y., Morais M.C., Cohen D.N., Bowman V.D., Anderson D.L.,
RA Rossmann M.G.;
RT "Crystal and cryoEM structural studies of a cell wall degrading enzyme in
RT the bacteriophage phi29 tail.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:9552-9557(2008).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), SUBUNIT, FUNCTION, AND SUBCELLULAR
RP LOCATION.
RX PubMed=27309813; DOI=10.1038/nature18017;
RA Xu J., Gui M., Wang D., Xiang Y.;
RT "The bacteriophage phi29 tail possesses a pore-forming loop for cell
RT membrane penetration.";
RL Nature 534:544-547(2016).
CC -!- FUNCTION: Distal (knob) tail protein that plugs the end of the tube
CC before DNA ejection and forms a channel perforating the host membrane
CC during ejection. {ECO:0000269|PubMed:18606992,
CC ECO:0000269|PubMed:27309813}.
CC -!- SUBUNIT: Homohexamer; forms a hexameric tube structure with six
CC flexible hydrophobic loops. {ECO:0000269|PubMed:27309813}.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:11562162,
CC ECO:0000269|PubMed:27309813, ECO:0000269|PubMed:8985375}. Note=Present
CC in 6 copies in the virion (PubMed:27309813). Located at the tip of the
CC tail and constitutes most of the tail knob (PubMed:18606992,
CC PubMed:27309813). {ECO:0000269|PubMed:11562162,
CC ECO:0000269|PubMed:18606992, ECO:0000269|PubMed:27309813}.
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DR EMBL; M14782; AAA32282.1; -; Genomic_DNA.
DR EMBL; EU771092; ACE96032.1; -; Genomic_DNA.
DR EMBL; M12456; AAA32291.1; -; Genomic_DNA.
DR PIR; D25816; WMBPT9.
DR RefSeq; YP_002004538.1; NC_011048.1.
DR PDB; 5FB4; X-ray; 2.04 A; A/B/C=1-599.
DR PDB; 5FB5; X-ray; 3.50 A; A/B=1-599.
DR PDB; 5FEI; X-ray; 2.60 A; A/B=1-599.
DR PDBsum; 5FB4; -.
DR PDBsum; 5FB5; -.
DR PDBsum; 5FEI; -.
DR SMR; P04331; -.
DR PRIDE; P04331; -.
DR GeneID; 6446507; -.
DR KEGG; vg:6446507; -.
DR Proteomes; UP000001207; Genome.
DR GO; GO:0098015; C:virus tail; IDA:UniProtKB.
DR GO; GO:0098026; C:virus tail, tube; IEA:UniProtKB-KW.
DR GO; GO:0044694; P:pore-mediated entry of viral genome into host cell; IEA:UniProtKB-KW.
DR GO; GO:0099002; P:viral genome ejection through host cell envelope, short tail mechanism; IEA:UniProtKB-KW.
DR InterPro; IPR031772; Caud_tail_N.
DR Pfam; PF16838; Caud_tail_N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Late protein;
KW Pore-mediated penetration of viral genome into host cell;
KW Reference proteome; Viral genome ejection through host cell envelope;
KW Viral penetration into host cytoplasm; Viral short tail ejection system;
KW Viral tail protein; Viral tail tube protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..599
FT /note="Tail knob protein gp9"
FT /id="PRO_0000106583"
FT CONFLICT 41
FT /note="R -> S (in Ref. 2; ACE96032)"
FT /evidence="ECO:0000305"
FT CONFLICT 121
FT /note="M -> I (in Ref. 2; ACE96032)"
FT /evidence="ECO:0000305"
FT STRAND 6..15
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 24..27
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 32..40
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 44..52
FT /evidence="ECO:0007829|PDB:5FB4"
FT TURN 56..59
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 69..72
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 77..81
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 83..85
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 90..101
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 104..111
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 113..117
FT /evidence="ECO:0007829|PDB:5FB4"
FT TURN 118..120
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 121..123
FT /evidence="ECO:0007829|PDB:5FB5"
FT STRAND 126..131
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 157..165
FT /evidence="ECO:0007829|PDB:5FB4"
FT TURN 167..170
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 172..180
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 199..205
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 207..214
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 222..225
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 235..244
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 246..251
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 252..259
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 266..268
FT /evidence="ECO:0007829|PDB:5FB4"
FT TURN 269..272
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 273..276
FT /evidence="ECO:0007829|PDB:5FB4"
FT TURN 278..280
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 281..286
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 289..291
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 294..299
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 308..314
FT /evidence="ECO:0007829|PDB:5FB4"
FT TURN 315..318
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 325..328
FT /evidence="ECO:0007829|PDB:5FB4"
FT TURN 330..332
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 333..338
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 340..342
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 344..347
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 349..351
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 352..362
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 366..368
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 371..374
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 386..392
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 393..396
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 397..400
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 413..419
FT /evidence="ECO:0007829|PDB:5FB5"
FT HELIX 423..425
FT /evidence="ECO:0007829|PDB:5FB5"
FT STRAND 429..432
FT /evidence="ECO:0007829|PDB:5FB5"
FT STRAND 463..465
FT /evidence="ECO:0007829|PDB:5FB5"
FT HELIX 469..479
FT /evidence="ECO:0007829|PDB:5FB5"
FT HELIX 493..495
FT /evidence="ECO:0007829|PDB:5FB5"
FT STRAND 496..498
FT /evidence="ECO:0007829|PDB:5FB5"
FT HELIX 500..505
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 510..518
FT /evidence="ECO:0007829|PDB:5FB4"
FT HELIX 520..533
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 535..541
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 548..558
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 561..565
FT /evidence="ECO:0007829|PDB:5FEI"
FT HELIX 567..579
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 581..584
FT /evidence="ECO:0007829|PDB:5FB4"
FT STRAND 586..588
FT /evidence="ECO:0007829|PDB:5FB4"
SQ SEQUENCE 599 AA; 67590 MW; 8A4F6649BFEE0536 CRC64;
MAYVPLSGTN VRILADVPFS NDYKNTRWFT SSSNQYNWFN RKSRVYEMSK VTFMGFRENK
PYVSVSLPID KLYSASYIMF QNADYGNKWF YAFVTELEFK NSAVTYVHFE IDVLQTWMFD
MKFQESFIVR EHVKLWNDDG TPTINTIDEG LSYGSEYDIV SVENHKPYDD MMFLVIISKS
IMHGTPGEEE SRLNDINASL NGMPQPLCYY IHPFYKDGKV PKTYIGDNNA NLSPIVNMLT
NIFSQKSAVN DIVNMYVTDY IGLKLDYKNG DKELKLDKDM FEQAGIADDK HGNVDTIFVK
KIPDYEALEI DTGDKWGGFT KDQESKLMMY PYCVTEITDF KGNHMNLKTE YINNSKLKIQ
VRGSLGVSNK VAYSVQDYNA DSALSGGNRL TASLDSSLIN NNPNDIAILN DYLSAYLQGN
KNSLENQKSS ILFNGIMGMI GGGISAGASA AGGSALGMAS SVTGMTSTAG NAVLQMQAMQ
AKQADIANIP PQLTKMGGNT AFDYGNGYRG VYVIKKQLKA EYRRSLSSFF HKYGYKINRV
KKPNLRTRKA FNYVQTKDCF ISGDINNNDL QEIRTIFDNG ITLWHTDNIG NYSVENELR
