DIT_BPLP2
ID DIT_BPLP2 Reviewed; 298 AA.
AC D3WAD3; D3KFX6;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 20-APR-2010, sequence version 1.
DT 02-JUN-2021, entry version 18.
DE RecName: Full=Distal tail protein {ECO:0000303|PubMed:20351260};
DE Short=Dit;
DE AltName: Full=Gene product 15 {ECO:0000305};
DE Short=Gp15 {ECO:0000305};
OS Lactococcus phage p2 (Lactococcus lactis bacteriophage p2).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Skunavirus.
OX NCBI_TaxID=254252;
OH NCBI_TaxID=1358; Lactococcus lactis.
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Tremblay D.M., Deveau H., Moineau S.;
RT "Complete genomic sequence of Lactococcus lactis phage p2.";
RL Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0007744|PDB:2WZP, ECO:0007744|PDB:2X53}
RP X-RAY CRYSTALLOGRAPHY (2.60 ANGSTROMS) OF 51-298, SUBUNIT, SUBCELLULAR
RP LOCATION, FUNCTION, AND INTERACTION WITH THE RECEPTOR BINDING PROTEIN.
RX PubMed=20351260; DOI=10.1073/pnas.1000232107;
RA Sciara G., Bebeacua C., Bron P., Tremblay D., Ortiz-Lombardia M.,
RA Lichiere J., van Heel M., Campanacci V., Moineau S., Cambillau C.;
RT "Structure of lactococcal phage p2 baseplate and its mechanism of
RT activation.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:6852-6857(2010).
CC -!- FUNCTION: Forms the distal part of the tail. Self-associates as two
CC rings organized back to back, with a central channel allowing DNA
CC ejection. {ECO:0000269|PubMed:20351260}.
CC -!- SUBUNIT: Homohexamer. Interacts with the receptor binding protein.
CC {ECO:0000269|PubMed:20351260}.
CC -!- INTERACTION:
CC D3WAD3; D3KFX4; NbExp=8; IntAct=EBI-15845172, EBI-15845184;
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:20351260}. Note=Part
CC of the tail. {ECO:0000269|PubMed:20351260}.
CC -!- SIMILARITY: Belongs to the skunalikevirus distal tail protein family.
CC {ECO:0000305}.
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DR EMBL; GQ979703; ADC80090.1; -; Genomic_DNA.
DR PDB; 2WZP; X-ray; 2.60 A; P/Q=2-298.
DR PDB; 2X53; X-ray; 3.90 A; S/T/U/V/W/X=2-298.
DR PDB; 6ZIG; EM; 42.20 A; 2/3/4/5/6/7/S/T/U/V/W/X=1-298.
DR PDB; 6ZIH; EM; 28.70 A; 2/3/4/5/6/7/S/T/U/V/W/X=1-298.
DR PDB; 6ZJJ; EM; 22.00 A; 2/3/4/5/6/7/S/T/U/V/W/X=1-298.
DR PDBsum; 2WZP; -.
DR PDBsum; 2X53; -.
DR PDBsum; 6ZIG; -.
DR PDBsum; 6ZIH; -.
DR PDBsum; 6ZJJ; -.
DR SMR; D3WAD3; -.
DR DIP; DIP-59523N; -.
DR IntAct; D3WAD3; 2.
DR Proteomes; UP000002348; Genome.
DR GO; GO:0098015; C:virus tail; IEA:UniProtKB-KW.
DR GO; GO:0099001; P:viral genome ejection through host cell envelope, long flexible tail mechanism; IEA:UniProtKB-KW.
DR InterPro; IPR031899; Baseplate_Gp15.
DR Pfam; PF16774; Baseplate; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome;
KW Viral genome ejection through host cell envelope;
KW Viral long flexible tail ejection system;
KW Viral penetration into host cytoplasm; Viral tail protein; Virion;
KW Virus entry into host cell.
FT CHAIN 1..298
FT /note="Distal tail protein"
FT /id="PRO_0000438225"
FT STRAND 4..10
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 18..20
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 21..23
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 24..29
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 43..45
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 48..50
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 55..57
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 61..67
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 72..87
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 92..97
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 102..112
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 120..122
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 125..145
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 154..156
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 160..163
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 172..174
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 184..188
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 190..192
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 196..205
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 212..218
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 223..229
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 236..241
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 248..253
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 256..259
FT /evidence="ECO:0007829|PDB:2WZP"
FT HELIX 261..263
FT /evidence="ECO:0007829|PDB:2WZP"
FT TURN 266..268
FT /evidence="ECO:0007829|PDB:2WZP"
FT STRAND 276..294
FT /evidence="ECO:0007829|PDB:2WZP"
SQ SEQUENCE 298 AA; 34475 MW; 3682A87D09DDA993 CRC64;
MVRQYKIHTN LDGTDDKVWD VTNGKVRFYQ PSNLGLQSTN NIWQSNGIGV MGTRSITQPQ
IEFKLETFGE SLEENYQLMK DFVNDILSKK FVTLEYQTEI FQVYADLALA DVTKTEGYGK
NGTFSEKITF DIITKWYTYE NLTFDKIQNG KVIAGMSKIY GGTAPGNYKY IKGTSYTYYG
ESDIDRLSRW DIKEEIFSFM GILYPKLPKT PAGVRFLDDI GNEYTAIVFK TEQVQDYILI
NTDVNDETYQ GWKGTTALNL FPVMDFERYR TRIIEKGQME LINLSKAEFK IKRKADFV
