ID   DIUH2_MANSE             Reviewed;          30 AA.
AC   P24858;
DT   01-MAR-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-1992, sequence version 1.
DT   25-MAY-2022, entry version 67.
DE   RecName: Full=Diuretic hormone 2;
DE            Short=DH-2;
DE   AltName: Full=DPII;
DE   AltName: Full=Diuresin {ECO:0000303|PubMed:10753561};
DE            Short=MD {ECO:0000303|PubMed:10753561};
DE   AltName: Full=Diuretic peptide 2;
DE            Short=DP-2;
OS   Manduca sexta (Tobacco hawkmoth) (Tobacco hornworm).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Lepidoptera; Glossata; Ditrysia; Bombycoidea;
OC   Sphingidae; Sphinginae; Sphingini; Manduca.
OX   NCBI_TaxID=7130;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, AND AMIDATION AT VAL-30.
RC   TISSUE=Corpora cardiaca;
RX   PubMed=1764106; DOI=10.1016/0006-291x(91)92025-f;
RA   Blackburn M.B., Kingan T.G., Bodnar W., Shabanowitz J., Hunt D.F.,
RA   Kempe T., Wagner R.M., Raina A.K., Schnee M.E., Ma M.C.;
RT   "Isolation and identification of a new diuretic peptide from the tobacco
RT   hornworm, Manduca sexta.";
RL   Biochem. Biophys. Res. Commun. 181:927-932(1991).
RN   [2]
RP   POSSIBLE ROLE IN PLANT PROTECTION.
RX   PubMed=10753561; DOI=10.1006/gcen.1999.7435;
RA   Ma M., Emery S.B., Wong W.K., De Loof A.;
RT   "Effects of Manduca diuresin on neonates of the tobacco hornworm, Manduca
RT   sexta.";
RL   Gen. Comp. Endocrinol. 118:1-7(2000).
CC   -!- FUNCTION: Regulation of fluid secretion. {ECO:0000269|PubMed:1764106}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:1764106}.
CC   -!- MISCELLANEOUS: Prolonged ingestion of diuretic hormone 2 by M.sexta
CC       neonates results in reduced food consumption, slowed growth and reduced
CC       developmental rates. A high percentage of neonates fail to molt into
CC       second instar larvae and death usually follows shortly thereafter,
CC       suggesting the possible use of this insecticidal peptide in plant
CC       protection. {ECO:0000269|PubMed:10753561}.
CC   -!- SIMILARITY: Belongs to the sauvagine/corticotropin-releasing
CC       factor/urotensin I family. {ECO:0000305}.
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DR   PIR; JS0645; JS0645.
DR   AlphaFoldDB; P24858; -.
DR   SMR; P24858; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   InterPro; IPR000187; CRF.
DR   SMART; SM00039; CRF; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Hormone; Secreted.
FT   PEPTIDE         1..30
FT                   /note="Diuretic hormone 2"
FT                   /id="PRO_0000043597"
FT   MOD_RES         30
FT                   /note="Valine amide"
FT                   /evidence="ECO:0000269|PubMed:1764106"
SQ   SEQUENCE   30 AA;  3561 MW;  5C2D6BD2DD8A2717 CRC64;
     SFSVNPAVDI LQHRYMEKVA QNNRNFLNRV