ID   DIUX_APIME              Reviewed;          31 AA.
AC   P85830;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Diuretic hormone class 2;
DE   AltName: Full=Diuretic peptide;
DE            Short=DP;
DE   AltName: Full=GLDLGLSRGFSGSQAAKHLMGLAAANYAGGP-amide;
DE   Contains:
DE     RecName: Full=Brain peptide GLDLGLSRGFSGSQAA;
DE   Contains:
DE     RecName: Full=Brain peptide GLDLGLSRGFSGSQAAKH;
OS   Apis mellifera (Honeybee).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae;
OC   Apis.
OX   NCBI_TaxID=7460;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND AMIDATION AT PRO-31.
RC   TISSUE=Brain {ECO:0000269|PubMed:17068263};
RX   PubMed=17068263; DOI=10.1126/science.1124128;
RA   Hummon A.B., Richmond T.A., Verleyen P., Baggerman G., Huybrechts J.,
RA   Ewing M.A., Vierstraete E., Rodriguez-Zas S.L., Schoofs L., Robinson G.E.,
RA   Sweedler J.V.;
RT   "From the genome to the proteome: uncovering peptides in the Apis brain.";
RL   Science 314:647-649(2006).
CC   -!- FUNCTION: Regulation of fluid secretion. Stimulates primary urine
CC       secretion by Malpighian tubules and causes a dose-dependent stimulation
CC       of cAMP levels in the tubules. Has a nonselective effect on Na(+)/K(+)
CC       ion transport. In vitro, primarily elevates intracellular Ca(2+) (By
CC       similarity). {ECO:0000250|UniProtKB:P82372}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:P82372}.
CC   -!- MASS SPECTROMETRY: [Diuretic hormone class 2]: Mass=2985.52;
CC       Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC   -!- MASS SPECTROMETRY: [Diuretic hormone class 2]: Mass=2985.52;
CC       Method=MALDI; Evidence={ECO:0000269|PubMed:17068263};
CC   -!- MASS SPECTROMETRY: [Brain peptide GLDLGLSRGFSGSQAA]: Mass=1534.76;
CC       Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC   -!- MASS SPECTROMETRY: [Brain peptide GLDLGLSRGFSGSQAAKH]: Mass=1799.93;
CC       Method=Electrospray; Evidence={ECO:0000269|PubMed:17068263};
CC   -!- SIMILARITY: Belongs to the diuretic hormone class 2 family.
CC       {ECO:0000305}.
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DR   AlphaFoldDB; P85830; -.
DR   STRING; 7460.GB45734-PA; -.
DR   PaxDb; P85830; -.
DR   eggNOG; ENOG502SAVV; Eukaryota.
DR   Proteomes; UP000005203; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Hormone; Reference proteome;
KW   Secreted.
FT   PEPTIDE         1..31
FT                   /note="Diuretic hormone class 2"
FT                   /id="PRO_0000339295"
FT   PEPTIDE         1..18
FT                   /note="Brain peptide GLDLGLSRGFSGSQAAKH"
FT                   /id="PRO_0000339297"
FT   PEPTIDE         1..16
FT                   /note="Brain peptide GLDLGLSRGFSGSQAA"
FT                   /id="PRO_0000339296"
FT   MOD_RES         31
FT                   /note="Proline amide"
FT                   /evidence="ECO:0000269|PubMed:17068263"
SQ   SEQUENCE   31 AA;  2988 MW;  0F311E0C3AD71A46 CRC64;
     GLDLGLSRGF SGSQAAKHLM GLAAANYAGG P