DIUX_DROME
ID DIUX_DROME Reviewed; 116 AA.
AC Q9VLK4;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Diuretic hormone class 2;
DE Short=Diuretic peptide;
DE AltName: Full=DH(31);
DE AltName: Full=DP;
DE Flags: Precursor;
GN Name=Dh31; ORFNames=CG13094;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PROTEIN SEQUENCE OF 76-106, IDENTIFICATION BY MASS SPECTROMETRY, MASS
RP SPECTROMETRY, AND AMIDATION AT PRO-106.
RC TISSUE=Midgut {ECO:0000303|PubMed:21214272};
RX PubMed=21214272; DOI=10.1021/pr101116g;
RA Reiher W., Shirras C., Kahnt J., Baumeister S., Isaac R.E., Wegener C.;
RT "Peptidomics and peptide hormone processing in the Drosophila midgut.";
RL J. Proteome Res. 10:1881-1892(2011).
RN [5]
RP FUNCTION, AND SYNTHESIS OF 76-106.
RX PubMed=11316500; DOI=10.1242/jeb.204.10.1795;
RA Coast G.M., Webster S.G., Schegg K.M., Tobe S.S., Schooley D.A.;
RT "The Drosophila melanogaster homologue of an insect calcitonin-like
RT diuretic peptide stimulates V-ATPase activity in fruit fly Malpighian
RT tubules.";
RL J. Exp. Biol. 204:1795-1804(2001).
CC -!- FUNCTION: Regulation of fluid secretion. Stimulates Malpighian tubules
CC fluid secretion by activating the apical membrane V-ATPase via cyclic
CC AMP of principal cells in the main secretory segment.
CC {ECO:0000269|PubMed:11316500}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- MASS SPECTROMETRY: Mass=3149.57; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:21214272};
CC -!- SIMILARITY: Belongs to the diuretic hormone class 2 family.
CC {ECO:0000305}.
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DR EMBL; AE014134; AAF52685.1; -; Genomic_DNA.
DR EMBL; BT012436; AAS93707.1; -; mRNA.
DR RefSeq; NP_523514.1; NM_078790.3.
DR AlphaFoldDB; Q9VLK4; -.
DR BioGRID; 60301; 1.
DR STRING; 7227.FBpp0079308; -.
DR PaxDb; Q9VLK4; -.
DR DNASU; 34181; -.
DR EnsemblMetazoa; FBtr0079699; FBpp0079307; FBgn0032048.
DR GeneID; 34181; -.
DR KEGG; dme:Dmel_CG13094; -.
DR CTD; 34181; -.
DR FlyBase; FBgn0032048; Dh31.
DR VEuPathDB; VectorBase:FBgn0032048; -.
DR eggNOG; ENOG502SAVV; Eukaryota.
DR HOGENOM; CLU_152129_0_0_1; -.
DR InParanoid; Q9VLK4; -.
DR BioGRID-ORCS; 34181; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 34181; -.
DR PRO; PR:Q9VLK4; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0032048; Expressed in brain and 13 other tissues.
DR ExpressionAtlas; Q9VLK4; baseline and differential.
DR Genevisible; Q9VLK4; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0008613; F:diuretic hormone activity; IDA:FlyBase.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IPI:FlyBase.
DR GO; GO:0007189; P:adenylate cyclase-activating G protein-coupled receptor signaling pathway; IDA:FlyBase.
DR GO; GO:0007589; P:body fluid secretion; IDA:FlyBase.
DR GO; GO:0007619; P:courtship behavior; IMP:FlyBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IDA:FlyBase.
DR GO; GO:2000252; P:negative regulation of feeding behavior; IMP:FlyBase.
DR GO; GO:0010841; P:positive regulation of circadian sleep/wake cycle, wakefulness; IMP:FlyBase.
DR GO; GO:0009266; P:response to temperature stimulus; IMP:FlyBase.
DR InterPro; IPR034439; DH2-like.
DR PANTHER; PTHR41146; PTHR41146; 1.
PE 1: Evidence at protein level;
KW Amidation; Cleavage on pair of basic residues; Direct protein sequencing;
KW Hormone; Reference proteome; Secreted; Signal.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT PROPEP 26..75
FT /evidence="ECO:0000255"
FT /id="PRO_0000006257"
FT PEPTIDE 76..106
FT /note="Diuretic hormone class 2"
FT /evidence="ECO:0000269|PubMed:21214272"
FT /id="PRO_0000006258"
FT PROPEP 112..116
FT /evidence="ECO:0000255"
FT /id="PRO_0000006259"
FT MOD_RES 106
FT /note="Proline amide"
FT /evidence="ECO:0000269|PubMed:21214272"
SQ SEQUENCE 116 AA; 12622 MW; 1BE68AA1EAA8FA49 CRC64;
MTNRCACFAL AFLLFCLLAI SSIEAAPMPS QSNGGYGGAG YNELEEVPDD LLMELMTRFG
RTIIRARNDL ENSKRTVDFG LARGYSGTQE AKHRMGLAAA NFAGGPGRRR RSETDV
