ID   DIUX_RHOPR              Reviewed;          31 AA.
AC   P85826; P86167;
DT   03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-MAR-2009, sequence version 1.
DT   25-MAY-2022, entry version 26.
DE   RecName: Full=Diuretic hormone class 2 {ECO:0000303|PubMed:19137558};
DE   AltName: Full=DH(31) {ECO:0000303|PubMed:19137558};
DE   AltName: Full=Diuretic peptide {ECO:0000250|UniProtKB:P82372};
DE            Short=DP {ECO:0000250|UniProtKB:P82372};
DE   AltName: Full=Rhopr-DH31 {ECO:0000303|PubMed:19137558};
DE   Contains:
DE     RecName: Full=Diuretic hormone class 2(1-16) {ECO:0000250|UniProtKB:P82372};
DE     AltName: Full=Rhopr-DH31(1-16) {ECO:0000303|PubMed:19137558};
OS   Rhodnius prolixus (Triatomid bug).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Paraneoptera; Hemiptera; Heteroptera; Panheteroptera;
OC   Cimicomorpha; Reduviidae; Triatominae; Rhodnius.
OX   NCBI_TaxID=13249;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND
RP   AMIDATION AT PRO-31.
RC   TISSUE=CNS;
RX   PubMed=18203994; DOI=10.1242/jeb.013771;
RA   Te Brugge V.A., Schooley D.A., Orchard I.;
RT   "Amino acid sequence and biological activity of a calcitonin-like diuretic
RT   hormone (DH31) from Rhodnius prolixus.";
RL   J. Exp. Biol. 211:382-390(2008).
RN   [2] {ECO:0000305}
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND AMIDATION AT PRO-31.
RC   TISSUE=Brain {ECO:0000269|PubMed:19137558};
RX   PubMed=19137558; DOI=10.1002/pmic.200800499;
RA   Ons S., Richter F., Urlaub H., Pomar R.R.;
RT   "The neuropeptidome of Rhodnius prolixus brain.";
RL   Proteomics 9:788-792(2009).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX   PubMed=15626502; DOI=10.1016/j.peptides.2004.08.025;
RA   Te Brugge V.A., Lombardi V.C., Schooley D.A., Orchard I.;
RT   "Presence and activity of a Dippu-DH31-like peptide in the blood-feeding
RT   bug, Rhodnius prolixus.";
RL   Peptides 26:29-42(2005).
CC   -!- FUNCTION: Regulation of fluid secretion. Stimulates primary urine
CC       secretion by the Malpighian tubules. Increases the frequency of
CC       contraction in the heart and hindgut. Causes a dose-dependent
CC       stimulation of cAMP levels in the dorsal vessel and hindgut, but not in
CC       Malpighian tubules. Has synergistic effects on urine secretion by
CC       Malpighian tubules with serotonin. {ECO:0000269|PubMed:15626502,
CC       ECO:0000269|PubMed:18203994}.
CC   -!- SUBCELLULAR LOCATION: Perikaryon {ECO:0000269|PubMed:15626502}. Cell
CC       projection, dendrite {ECO:0000269|PubMed:15626502}. Cell projection,
CC       axon {ECO:0000269|PubMed:15626502}. Secreted
CC       {ECO:0000269|PubMed:15626502}.
CC   -!- TISSUE SPECIFICITY: Detected throughout the central nervous system in
CC       fifth instar larvae, particularly in the medial and lateral
CC       neurosecretory cells and the dorsal unpaired median neurons of the
CC       mesothoracic ganglionic mass. Also detected in nerve processes over the
CC       salivary glands, dorsal hindgut, anterior dorsal vessel and dorsal
CC       diaphragm, and at the neurohemal sites of the abdominal nerves. Not
CC       detected in the nerves directly innervating the heart, alary muscles or
CC       posterior dorsal vessel. One hour after feeding levels on the dorsal
CC       surface of the mesothoracic ganglionic mass, the neurohemal sites and
CC       the processes above the dorsal hindgut are much reduced while
CC       background levels are increased (at protein level).
CC       {ECO:0000269|PubMed:15626502, ECO:0000269|PubMed:18203994}.
CC   -!- MASS SPECTROMETRY: [Diuretic hormone class 2]: Mass=2986.61;
CC       Method=MALDI; Evidence={ECO:0000269|PubMed:18203994,
CC       ECO:0000269|PubMed:19137558};
CC   -!- MASS SPECTROMETRY: [Diuretic hormone class 2]: Mass=2986.5;
CC       Method=MALDI; Evidence={ECO:0000269|PubMed:19137558};
CC   -!- MASS SPECTROMETRY: [Diuretic hormone class 2(1-16)]: Mass=1535.79;
CC       Method=MALDI; Evidence={ECO:0000269|PubMed:19137558};
CC   -!- SIMILARITY: Belongs to the diuretic hormone class 2 family.
CC       {ECO:0000255}.
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DR   AlphaFoldDB; P85826; -.
DR   Proteomes; UP000015103; Unassembled WGS sequence.
DR   GO; GO:0030424; C:axon; IDA:UniProtKB.
DR   GO; GO:0030425; C:dendrite; IDA:UniProtKB.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0043204; C:perikaryon; IDA:UniProtKB.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0007589; P:body fluid secretion; IDA:UniProtKB.
DR   GO; GO:0035810; P:positive regulation of urine volume; IDA:UniProtKB.
DR   GO; GO:0008016; P:regulation of heart contraction; IDA:UniProtKB.
DR   GO; GO:0043134; P:regulation of hindgut contraction; IDA:UniProtKB.
PE   1: Evidence at protein level;
KW   Amidation; Cell projection; Direct protein sequencing; Hormone;
KW   Reference proteome; Secreted.
FT   PEPTIDE         1..31
FT                   /note="Diuretic hormone class 2"
FT                   /evidence="ECO:0000269|PubMed:19137558"
FT                   /id="PRO_0000364023"
FT   PEPTIDE         1..16
FT                   /note="Diuretic hormone class 2(1-16)"
FT                   /evidence="ECO:0000269|PubMed:19137558"
FT                   /id="PRO_0000365761"
FT   MOD_RES         31
FT                   /note="Proline amide"
FT                   /evidence="ECO:0000269|PubMed:18203994,
FT                   ECO:0000269|PubMed:19137558"
SQ   SEQUENCE   31 AA;  2988 MW;  0F311E0C3AD71A46 CRC64;
     GLDLGLSRGF SGSQAAKHLM GLAAANYAGG P