DIV4A_BACSU
ID DIV4A_BACSU Reviewed; 164 AA.
AC P71021; O08052; Q796I7;
DT 20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=Septum site-determining protein DivIVA;
DE AltName: Full=Cell division initiation protein DivIVA;
DE AltName: Full=Minicell-associated protein DivIVA;
GN Name=divIVA; Synonyms=ylmJ; OrderedLocusNames=BSU15420;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTAGENESIS OF ALA-78 AND
RP 157-ASP--GLU-164, AND DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=9045828; DOI=10.1128/jb.179.5.1671-1683.1997;
RA Cha J.-H., Stewart G.C.;
RT "The divIVA minicell locus of Bacillus subtilis.";
RL J. Bacteriol. 179:1671-1683(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION,
RP INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=168;
RX PubMed=9219999; DOI=10.1046/j.1365-2958.1997.3811764.x;
RA Edwards D.H., Errington J.;
RT "The Bacillus subtilis DivIVA protein targets to the division septum and
RT controls the site specificity of cell division.";
RL Mol. Microbiol. 24:905-915(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [4]
RP MUTAGENESIS OF PHE-17; ARG-18; GLY-19 AND TYR-20.
RX PubMed=15554965; DOI=10.1111/j.1365-2958.2004.04363.x;
RA Perry S.E., Edwards D.H.;
RT "Identification of a polar targeting determinant for Bacillus subtilis
RT DivIVA.";
RL Mol. Microbiol. 54:1237-1249(2004).
RN [5]
RP SUBUNIT, AND MUTAGENESIS OF LEU-121 AND GLU-162.
RX PubMed=11882716; DOI=10.1099/00221287-148-3-807;
RA Muchova K., Kutejova E., Scott D.J., Brannigan J.A., Lewis R.J.,
RA Wilkinson A.J., Barak I.;
RT "Oligomerization of the Bacillus subtilis division protein DivIVA.";
RL Microbiology 148:807-813(2002).
RN [6]
RP SUBUNIT.
RX PubMed=15165232; DOI=10.1111/j.1365-2958.2004.04074.x;
RA Stahlberg H., Kutejova E., Muchova K., Gregorini M., Lustig A.,
RA Mueller S.A., Olivieri V., Engel A., Wilkinson A.J., Barak I.;
RT "Oligomeric structure of the Bacillus subtilis cell division protein DivIVA
RT determined by transmission electron microscopy.";
RL Mol. Microbiol. 52:1281-1290(2004).
RN [7]
RP SUBCELLULAR LOCATION, AND INTERACTION WITH FTSZ; MIND AND SPO0J.
RX PubMed=16885474; DOI=10.1128/jb.01750-05;
RA Perry S.E., Edwards D.H.;
RT "The Bacillus subtilis DivIVA protein has a sporulation-specific proximity
RT to Spo0J.";
RL J. Bacteriol. 188:6039-6043(2006).
RN [8]
RP INTERACTION WITH MINJ.
RC STRAIN=3610;
RX PubMed=18976281; DOI=10.1111/j.1365-2958.2008.06469.x;
RA Patrick J.E., Kearns D.B.;
RT "MinJ (YvjD) is a topological determinant of cell division in Bacillus
RT subtilis.";
RL Mol. Microbiol. 70:1166-1179(2008).
RN [9]
RP INTERACTION WITH MINJ.
RC STRAIN=168;
RX PubMed=19019154; DOI=10.1111/j.1365-2958.2008.06501.x;
RA Bramkamp M., Emmins R., Weston L., Donovan C., Daniel R.A., Errington J.;
RT "A novel component of the division-site selection system of Bacillus
RT subtilis and a new mode of action for the division inhibitor MinCD.";
RL Mol. Microbiol. 70:1556-1569(2008).
CC -!- FUNCTION: May act as a pilot protein, directing MinCD to the polar
CC septation sites or by inhibiting MinCD at the midcell site of division.
CC Required for polar localization of the chromosome during sporulation.
CC {ECO:0000269|PubMed:9045828, ECO:0000269|PubMed:9219999}.
CC -!- SUBUNIT: Oligomers. Interacts with FtsZ, MinD, MinJ and Spo0J. The
CC association with Spo0J is transient and occurs at a specific point
CC during development. {ECO:0000269|PubMed:11882716,
CC ECO:0000269|PubMed:15165232, ECO:0000269|PubMed:16885474,
CC ECO:0000269|PubMed:18976281, ECO:0000269|PubMed:19019154}.
CC -!- INTERACTION:
CC P71021; O32049: comN; NbExp=4; IntAct=EBI-5243654, EBI-6418652;
CC P71021; P71021: divIVA; NbExp=10; IntAct=EBI-5243654, EBI-5243654;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16885474,
CC ECO:0000269|PubMed:9219999}. Note=Localized at the cell division site
CC and found exclusively at the cell pole during sporulation.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:9219999}.
CC -!- DISRUPTION PHENOTYPE: Misplacement of the septum during cell division,
CC resulting in the formation of small, circular, anucleate minicells.
CC {ECO:0000269|PubMed:9045828, ECO:0000269|PubMed:9219999}.
CC -!- MISCELLANEOUS: Overexpression of DivIVA is lethal.
CC -!- SIMILARITY: Belongs to the DivIVA family. {ECO:0000305}.
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DR EMBL; U60901; AAB49279.1; -; Genomic_DNA.
DR EMBL; Z86114; CAB06818.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB13416.1; -; Genomic_DNA.
DR PIR; B69616; B69616.
DR RefSeq; NP_389425.1; NC_000964.3.
DR RefSeq; WP_003221468.1; NZ_JNCM01000035.1.
DR PDB; 2WUJ; X-ray; 1.40 A; A/B=1-57.
DR PDB; 2WUK; X-ray; 1.90 A; A/B/C/D=1-57.
DR PDBsum; 2WUJ; -.
DR PDBsum; 2WUK; -.
DR AlphaFoldDB; P71021; -.
DR SMR; P71021; -.
DR IntAct; P71021; 11.
DR MINT; P71021; -.
DR STRING; 224308.BSU15420; -.
DR jPOST; P71021; -.
DR PaxDb; P71021; -.
DR PRIDE; P71021; -.
DR EnsemblBacteria; CAB13416; CAB13416; BSU_15420.
DR GeneID; 64303433; -.
DR GeneID; 939972; -.
DR KEGG; bsu:BSU15420; -.
DR PATRIC; fig|224308.179.peg.1680; -.
DR eggNOG; COG3599; Bacteria.
DR InParanoid; P71021; -.
DR OMA; QSIFVAQ; -.
DR PhylomeDB; P71021; -.
DR BioCyc; BSUB:BSU15420-MON; -.
DR EvolutionaryTrace; P71021; -.
DR PRO; PR:P71021; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0000917; P:division septum assembly; IEA:UniProtKB-KW.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR InterPro; IPR019933; DivIVA_domain.
DR InterPro; IPR007793; DivIVA_fam.
DR PANTHER; PTHR35794; PTHR35794; 1.
DR Pfam; PF05103; DivIVA; 1.
DR TIGRFAMs; TIGR03544; DivI1A_domain; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; Coiled coil; Cytoplasm;
KW Reference proteome; Septation; Sporulation.
FT CHAIN 1..164
FT /note="Septum site-determining protein DivIVA"
FT /id="PRO_0000360608"
FT COILED 29..107
FT /evidence="ECO:0000255"
FT MUTAGEN 17
FT /note="F->L: Diffuse localization."
FT /evidence="ECO:0000269|PubMed:15554965"
FT MUTAGEN 18
FT /note="R->A,C: Localization at cytoplasmic foci instead of
FT the cell pole. Stable interaction with Spo0J and
FT association with the chromosome."
FT /evidence="ECO:0000269|PubMed:15554965"
FT MUTAGEN 19
FT /note="G->A: Localized at cytoplasmic foci."
FT /evidence="ECO:0000269|PubMed:15554965"
FT MUTAGEN 20
FT /note="Y->C: No effect on localization."
FT /evidence="ECO:0000269|PubMed:15554965"
FT MUTAGEN 78
FT /note="A->T: In divIVA1; loss of function resulting in
FT sporulation defect and in the formation of minicells."
FT /evidence="ECO:0000269|PubMed:9045828"
FT MUTAGEN 121
FT /note="L->P: In divIVA2; loss of function."
FT /evidence="ECO:0000269|PubMed:11882716"
FT MUTAGEN 157..164
FT /note="DAVFEEKE->LQVSGVVI: No effect."
FT /evidence="ECO:0000269|PubMed:9045828"
FT MUTAGEN 162
FT /note="E->K: No effect."
FT /evidence="ECO:0000269|PubMed:11882716"
FT HELIX 5..8
FT /evidence="ECO:0007829|PDB:2WUJ"
FT STRAND 19..21
FT /evidence="ECO:0007829|PDB:2WUJ"
FT HELIX 22..51
FT /evidence="ECO:0007829|PDB:2WUJ"
SQ SEQUENCE 164 AA; 19341 MW; B62978CA02708F68 CRC64;
MPLTPNDIHN KTFTKSFRGY DEDEVNEFLA QVRKDYEIVL RKKTELEAKV NELDERIGHF
ANIEETLNKS ILVAQEAAED VKRNSQKEAK LIVREAEKNA DRIINESLSK SRKIAMEIEE
LKKQSKVFRT RFQMLIEAQL DLLKNDDWDH LLEYEVDAVF EEKE
