DJ1A_ARATH
ID DJ1A_ARATH Reviewed; 392 AA.
AC Q9FPF0; Q9LKA6;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Protein DJ-1 homolog A;
DE Short=AtDJ1A;
GN Name=DJ1A; OrderedLocusNames=At3g14990; ORFNames=K15M2.13;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia; TISSUE=Rosette leaf;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=cv. Columbia;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP INDUCTION BY CIS-JASMONE.
RX PubMed=18356298; DOI=10.1073/pnas.0710305105;
RA Bruce T.J., Matthes M.C., Chamberlain K., Woodcock C.M., Mohib A.,
RA Webster B., Smart L.E., Birkett M.A., Pickett J.A., Napier J.A.;
RT "cis-Jasmone induces Arabidopsis genes that affect the chemical ecology of
RT multitrophic interactions with aphids and their parasitoids.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:4553-4558(2008).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH CSD1 AND GPX2, INDUCTION,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=20406884; DOI=10.1242/jcs.063222;
RA Xu X.M., Lin H., Maple J., Bjoerkblom B., Alves G., Larsen J.P.,
RA Moeller S.G.;
RT "The Arabidopsis DJ-1a protein confers stress protection through cytosolic
RT SOD activation.";
RL J. Cell Sci. 123:1644-1651(2010).
RN [7]
RP SUBUNIT.
RX PubMed=23651081; DOI=10.1111/febs.12321;
RA Kwon K., Choi D., Hyun J.K., Jung H.S., Baek K., Park C.;
RT "Novel glyoxalases from Arabidopsis thaliana.";
RL FEBS J. 280:3328-3339(2013).
CC -!- FUNCTION: Involved in oxidative stress response. Confers protection
CC against diverse stresses by binding both CSD1 and GPX2 and mediating
CC the cytosolic activation of the Cu-Zn-dependent superoxide dismutase
CC activity of CSD1. {ECO:0000269|PubMed:20406884}.
CC -!- SUBUNIT: Homodimer (Probable). Interacts with CSD1 and GPX2.
CC {ECO:0000269|PubMed:20406884, ECO:0000269|PubMed:23651081,
CC ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:20406884}.
CC Nucleus {ECO:0000269|PubMed:20406884}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9FPF0-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9FPF0-2; Sequence=VSP_046013;
CC -!- INDUCTION: By high light, copper, hydrogen peroxide, methyl viologen
CC and cis-jasmone, but not methyl jasmonate.
CC {ECO:0000269|PubMed:18356298, ECO:0000269|PubMed:20406884}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but mutant plants have increased susceptibility to
CC oxidative stress-induced cell death and accelerated cell death in aging
CC plants. {ECO:0000269|PubMed:20406884}.
CC -!- SIMILARITY: Belongs to the peptidase C56 family. {ECO:0000305}.
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DR EMBL; AP000370; BAA97062.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75598.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75599.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75600.1; -; Genomic_DNA.
DR EMBL; AF326856; AAG41438.1; -; mRNA.
DR EMBL; AF349515; AAK15562.1; -; mRNA.
DR EMBL; AY039574; AAK62629.1; -; mRNA.
DR EMBL; AY129490; AAM91076.1; -; mRNA.
DR EMBL; AK317457; BAH20124.1; -; mRNA.
DR RefSeq; NP_001030698.1; NM_001035621.1. [Q9FPF0-2]
DR RefSeq; NP_188117.1; NM_112361.5. [Q9FPF0-1]
DR RefSeq; NP_850588.1; NM_180257.1. [Q9FPF0-2]
DR AlphaFoldDB; Q9FPF0; -.
DR SMR; Q9FPF0; -.
DR BioGRID; 6062; 7.
DR IntAct; Q9FPF0; 2.
DR STRING; 3702.AT3G14990.1; -.
DR iPTMnet; Q9FPF0; -.
DR MetOSite; Q9FPF0; -.
DR PaxDb; Q9FPF0; -.
DR PRIDE; Q9FPF0; -.
DR ProteomicsDB; 222206; -. [Q9FPF0-1]
DR EnsemblPlants; AT3G14990.1; AT3G14990.1; AT3G14990. [Q9FPF0-1]
DR EnsemblPlants; AT3G14990.2; AT3G14990.2; AT3G14990. [Q9FPF0-2]
DR EnsemblPlants; AT3G14990.3; AT3G14990.3; AT3G14990. [Q9FPF0-2]
DR GeneID; 820728; -.
DR Gramene; AT3G14990.1; AT3G14990.1; AT3G14990. [Q9FPF0-1]
DR Gramene; AT3G14990.2; AT3G14990.2; AT3G14990. [Q9FPF0-2]
DR Gramene; AT3G14990.3; AT3G14990.3; AT3G14990. [Q9FPF0-2]
DR KEGG; ath:AT3G14990; -.
DR Araport; AT3G14990; -.
DR TAIR; locus:2086295; AT3G14990.
DR eggNOG; KOG2764; Eukaryota.
DR HOGENOM; CLU_000445_44_0_1; -.
DR InParanoid; Q9FPF0; -.
DR OMA; ISDCANT; -.
DR PhylomeDB; Q9FPF0; -.
DR BRENDA; 4.2.1.130; 399.
DR PRO; PR:Q9FPF0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9FPF0; baseline and differential.
DR Genevisible; Q9FPF0; AT.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0019172; F:glyoxalase III activity; IDA:TAIR.
DR GO; GO:1903189; P:glyoxal metabolic process; IBA:GO_Central.
DR GO; GO:1900409; P:positive regulation of cellular response to oxidative stress; IDA:UniProtKB.
DR Gene3D; 3.40.50.880; -; 2.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR006287; DJ-1.
DR InterPro; IPR002818; DJ-1/PfpI.
DR Pfam; PF01965; DJ-1_PfpI; 2.
DR SUPFAM; SSF52317; SSF52317; 2.
DR TIGRFAMs; TIGR01383; not_thiJ; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Nucleus; Reference proteome; Repeat;
KW Stress response.
FT CHAIN 1..392
FT /note="Protein DJ-1 homolog A"
FT /id="PRO_0000421813"
FT DOMAIN 6..174
FT /note="PfpI endopeptidase 1"
FT DOMAIN 212..378
FT /note="PfpI endopeptidase 2"
FT VAR_SEQ 1..23
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046013"
SQ SEQUENCE 392 AA; 41857 MW; A29CE52C5E3D090A CRC64;
MASFTKTVLI PIAHGTEPLE AVAMITVLRR GGADVTVASV ETQVGVDACH GIKMVADTLL
SDITDSVFDL IVLPGGLPGG ETLKNCKSLE NMVKKQDSDG RLNAAICCAP ALALGTWGLL
EGKKATGYPV FMEKLAATCA TAVESRVQID GRIVTSRGPG TTIEFSITLI EQLFGKEKAD
EVSSILLLRP NPGEEFTFTE LNQTNWSFED TPQILVPIAE ESEEIEAIAL VDILRRAKAN
VVIAAVGNSL EVEGSRKAKL VAEVLLDEVA EKSFDLIVLP GGLNGAQRFA SCEKLVNMLR
KQAEANKPYG GICASPAYVF EPNGLLKGKK ATTHPVVSDK LSDKSHIEHR VVVDGNVITS
RAPGTAMEFS LAIVEKFYGR EKALQLGKAT LV
