DJ1A_DROME
ID DJ1A_DROME Reviewed; 217 AA.
AC A1Z9J4;
DT 15-MAR-2017, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Protein DJ-1alpha {ECO:0000305};
GN Name=DJ-1alpha {ECO:0000312|FlyBase:FBgn0033885};
GN ORFNames=CG6646 {ECO:0000312|FlyBase:FBgn0033885};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=16139213; DOI=10.1016/j.cub.2005.07.064;
RA Meulener M., Whitworth A.J., Armstrong-Gold C.E., Rizzu P., Heutink P.,
RA Wes P.D., Pallanck L.J., Bonini N.M.;
RT "Drosophila DJ-1 mutants are selectively sensitive to environmental toxins
RT associated with Parkinson's disease.";
RL Curr. Biol. 15:1572-1577(2005).
RN [4]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=16139214; DOI=10.1016/j.cub.2005.07.036;
RA Menzies F.M., Yenisetti S.C., Min K.T.;
RT "Roles of Drosophila DJ-1 in survival of dopaminergic neurons and oxidative
RT stress.";
RL Curr. Biol. 15:1578-1582(2005).
RN [5]
RP SUBCELLULAR LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=16203113; DOI=10.1016/j.gene.2005.06.040;
RA Park J., Kim S.Y., Cha G.H., Lee S.B., Kim S., Chung J.;
RT "Drosophila DJ-1 mutants show oxidative stress-sensitive locomotive
RT dysfunction.";
RL Gene 361:133-139(2005).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20457924; DOI=10.1073/pnas.0911175107;
RA Hao L.Y., Giasson B.I., Bonini N.M.;
RT "DJ-1 is critical for mitochondrial function and rescues PINK1 loss of
RT function.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:9747-9752(2010).
CC -!- FUNCTION: Plays an important role in cell protection against oxidative
CC stress and cell death acting as oxidative stress sensor
CC (PubMed:16139213, PubMed:16139214, PubMed:20457924). Does not play a
CC role in methylglyoxal detoxification (By similarity).
CC {ECO:0000250|UniProtKB:Q99497, ECO:0000250|UniProtKB:Q9VA37,
CC ECO:0000269|PubMed:16139213, ECO:0000269|PubMed:16139214,
CC ECO:0000269|PubMed:20457924}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16203113}. Nucleus
CC {ECO:0000269|PubMed:16203113}. Mitochondrion
CC {ECO:0000269|PubMed:16203113}.
CC -!- TISSUE SPECIFICITY: Expressed in testis (at protein level).
CC {ECO:0000269|PubMed:16139213, ECO:0000269|PubMed:16139214,
CC ECO:0000269|PubMed:16203113}.
CC -!- DEVELOPMENTAL STAGE: Expressed at high levels only in the later stages
CC of development. {ECO:0000269|PubMed:16139214}.
CC -!- DISRUPTION PHENOTYPE: Double knockouts for dj-1beta and dj-1alpha is
CC viable but with reduced male fertility (PubMed:16139213,
CC PubMed:20457924). Double knockouts for dj-1beta and dj-1alpha is more
CC sensitive to chemical agents that induce oxidative stress
CC (PubMed:16139213). Double knockouts for dj-1beta and dj-1alpha shows
CC reduced lifespan and decreased spontaneous movement over time
CC (PubMed:20457924). Double knockouts for dj-1beta and dj-1alpha
CC spermatozoa are morphologically normal but immotile with abnormal
CC vacuoles in the Nebenkern, abnormal mitochondria and aberrant
CC separation of investment cones during sperm individualization
CC (PubMed:20457924). {ECO:0000269|PubMed:16139213,
CC ECO:0000269|PubMed:20457924}.
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DR EMBL; AE013599; AAF58316.2; -; Genomic_DNA.
DR RefSeq; NP_610916.1; NM_137072.1.
DR AlphaFoldDB; A1Z9J4; -.
DR SMR; A1Z9J4; -.
DR STRING; 7227.FBpp0086741; -.
DR MEROPS; C56.002; -.
DR PaxDb; A1Z9J4; -.
DR PRIDE; A1Z9J4; -.
DR EnsemblMetazoa; FBtr0087615; FBpp0086741; FBgn0033885.
DR GeneID; 36543; -.
DR KEGG; dme:Dmel_CG6646; -.
DR UCSC; CG6646-RA; d. melanogaster.
DR CTD; 36543; -.
DR FlyBase; FBgn0033885; DJ-1alpha.
DR VEuPathDB; VectorBase:FBgn0033885; -.
DR eggNOG; KOG2764; Eukaryota.
DR GeneTree; ENSGT00390000001231; -.
DR HOGENOM; CLU_000445_44_2_1; -.
DR InParanoid; A1Z9J4; -.
DR OMA; CSGDLWQ; -.
DR OrthoDB; 1165707at2759; -.
DR PhylomeDB; A1Z9J4; -.
DR BioGRID-ORCS; 36543; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36543; -.
DR PRO; PR:A1Z9J4; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0033885; Expressed in testis and 10 other tissues.
DR ExpressionAtlas; A1Z9J4; baseline and differential.
DR Genevisible; A1Z9J4; DM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0051920; F:peroxiredoxin activity; ISS:FlyBase.
DR GO; GO:0036524; F:protein deglycase activity; ISS:FlyBase.
DR GO; GO:0042417; P:dopamine metabolic process; IMP:FlyBase.
DR GO; GO:0046295; P:glycolate biosynthetic process; IBA:GO_Central.
DR GO; GO:1903189; P:glyoxal metabolic process; IBA:GO_Central.
DR GO; GO:0042775; P:mitochondrial ATP synthesis coupled electron transport; IGI:FlyBase.
DR GO; GO:1901215; P:negative regulation of neuron death; IBA:GO_Central.
DR GO; GO:0070050; P:neuron cellular homeostasis; IMP:FlyBase.
DR GO; GO:0014065; P:phosphatidylinositol 3-kinase signaling; IGI:FlyBase.
DR GO; GO:0051896; P:regulation of protein kinase B signaling; IMP:FlyBase.
DR GO; GO:0006979; P:response to oxidative stress; IDA:FlyBase.
DR Gene3D; 3.40.50.880; -; 1.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR006287; DJ-1.
DR InterPro; IPR002818; DJ-1/PfpI.
DR Pfam; PF01965; DJ-1_PfpI; 1.
DR SUPFAM; SSF52317; SSF52317; 1.
DR TIGRFAMs; TIGR01383; not_thiJ; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Mitochondrion; Nucleus; Oxidation; Reference proteome.
FT CHAIN 1..217
FT /note="Protein DJ-1alpha"
FT /id="PRO_0000439171"
FT ACT_SITE 133
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:Q99497"
FT MOD_RES 133
FT /note="Cysteine sulfinic acid (-SO2H); alternate"
FT /evidence="ECO:0000250|UniProtKB:Q9VA37"
SQ SEQUENCE 217 AA; 23173 MW; 78836E7B28181382 CRC64;
MLSVLRKSFP NGVTHAHRVI RCKSNQDKCA KNALIILAPG AEEMEFTISA DVLRRGKILV
TVAGLHDCEP VKCSRSVVIV PDTSLEEAVT RGDYDVVVLP GGLAGNKALM NSSAVGDVLR
CQESKGGLIA AICAAPTALA KHGIGKGKSI TSHPDMKPQL KELYCYIDDK TVVQDGNIIT
SRGPGTTFDF ALKITEQLVG AEVAKEVAKA MLWTYKP
