DJC25_HUMAN
ID DJC25_HUMAN Reviewed; 360 AA.
AC Q9H1X3; Q5QTD8; Q96BN9;
DT 02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=DnaJ homolog subfamily C member 25;
GN Name=DNAJC25;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Prostate;
RA Zan Q., Guo J.H., Yu L.;
RL Submitted (NOV-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RA Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S.,
RA Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y.,
RA Phelan M., Farmer A.;
RT "Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RC TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=24275569; DOI=10.1016/j.jprot.2013.11.014;
RA Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L.,
RA Ye M., Zou H.;
RT "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver
RT phosphoproteome.";
RL J. Proteomics 96:253-262(2014).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9H1X3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9H1X3-2; Sequence=VSP_035180;
CC Name=3;
CC IsoId=Q9H1X3-3; Sequence=VSP_035181, VSP_035182;
CC -!- SIMILARITY: Belongs to the DNAJC25 family. {ECO:0000305}.
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DR EMBL; AF447177; AAQ04643.1; -; mRNA.
DR EMBL; AK095452; BAG53057.1; -; mRNA.
DR EMBL; BT007068; AAP35731.1; -; mRNA.
DR EMBL; AL135787; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471105; EAW59082.1; -; Genomic_DNA.
DR EMBL; CH471105; EAW59085.1; -; Genomic_DNA.
DR EMBL; BC015391; AAH15391.1; -; mRNA.
DR EMBL; BC048318; AAH48318.1; -; mRNA.
DR CCDS; CCDS43862.1; -. [Q9H1X3-1]
DR RefSeq; NP_001015882.2; NM_001015882.2. [Q9H1X3-1]
DR RefSeq; NP_004116.2; NM_004125.3. [Q9H1X3-3]
DR AlphaFoldDB; Q9H1X3; -.
DR SMR; Q9H1X3; -.
DR BioGRID; 139240; 283.
DR BioGRID; 139267; 36.
DR IntAct; Q9H1X3; 2.
DR STRING; 9606.ENSP00000320650; -.
DR iPTMnet; Q9H1X3; -.
DR PhosphoSitePlus; Q9H1X3; -.
DR BioMuta; DNAJC25; -.
DR DMDM; 74752592; -.
DR EPD; Q9H1X3; -.
DR jPOST; Q9H1X3; -.
DR MassIVE; Q9H1X3; -.
DR MaxQB; Q9H1X3; -.
DR PaxDb; Q9H1X3; -.
DR PeptideAtlas; Q9H1X3; -.
DR PRIDE; Q9H1X3; -.
DR ProteomicsDB; 80452; -. [Q9H1X3-1]
DR ProteomicsDB; 80453; -. [Q9H1X3-2]
DR ProteomicsDB; 80454; -. [Q9H1X3-3]
DR Antibodypedia; 15144; 101 antibodies from 18 providers.
DR DNASU; 548645; -.
DR Ensembl; ENST00000313525.4; ENSP00000320650.3; ENSG00000059769.20. [Q9H1X3-1]
DR GeneID; 548645; -.
DR GeneID; 552891; -.
DR KEGG; hsa:548645; -.
DR KEGG; hsa:552891; -.
DR MANE-Select; ENST00000313525.4; ENSP00000320650.3; NM_001015882.3; NP_001015882.2.
DR UCSC; uc004bfl.3; human. [Q9H1X3-1]
DR CTD; 548645; -.
DR CTD; 552891; -.
DR DisGeNET; 548645; -.
DR DisGeNET; 552891; -.
DR GeneCards; DNAJC25; -.
DR HGNC; HGNC:34187; DNAJC25.
DR HPA; ENSG00000059769; Tissue enriched (liver).
DR neXtProt; NX_Q9H1X3; -.
DR OpenTargets; ENSG00000059769; -.
DR PharmGKB; PA165585764; -.
DR VEuPathDB; HostDB:ENSG00000059769; -.
DR eggNOG; KOG0722; Eukaryota.
DR GeneTree; ENSGT00390000013355; -.
DR HOGENOM; CLU_055735_0_0_1; -.
DR InParanoid; Q9H1X3; -.
DR OMA; IGKAYRQ; -.
DR OrthoDB; 1332758at2759; -.
DR PhylomeDB; Q9H1X3; -.
DR TreeFam; TF313563; -.
DR PathwayCommons; Q9H1X3; -.
DR SignaLink; Q9H1X3; -.
DR BioGRID-ORCS; 548645; 9 hits in 1057 CRISPR screens.
DR BioGRID-ORCS; 552891; 2 hits in 129 CRISPR screens.
DR ChiTaRS; DNAJC25; human.
DR Pharos; Q9H1X3; Tdark.
DR PRO; PR:Q9H1X3; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q9H1X3; protein.
DR Bgee; ENSG00000059769; Expressed in epithelial cell of pancreas and 176 other tissues.
DR ExpressionAtlas; Q9H1X3; baseline and differential.
DR Genevisible; Q9H1X3; HS.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR044632; DNAJC25-like.
DR InterPro; IPR036869; J_dom_sf.
DR PANTHER; PTHR44176; PTHR44176; 1.
DR Pfam; PF00226; DnaJ; 1.
DR PRINTS; PR00625; JDOMAIN.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chaperone; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..360
FT /note="DnaJ homolog subfamily C member 25"
FT /id="PRO_0000348569"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..170
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 49..124
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT VAR_SEQ 1..122
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039, ECO:0000303|Ref.1"
FT /id="VSP_035180"
FT VAR_SEQ 113..153
FT /note="DEETRKDYDYMLDHPEEYYSHYYHYYSRRLAPKVDVRVVIL -> VSQAAAE
FT LQQYCMQNACKDALLVGVPAGSNPFREPRSCALL (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.3"
FT /id="VSP_035181"
FT VAR_SEQ 154..360
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:15489334, ECO:0000303|Ref.3"
FT /id="VSP_035182"
SQ SEQUENCE 360 AA; 42404 MW; 6D6673C4B71ABD19 CRC64;
MGAPLLSPGW GAGAAGRRWW MLLAPLLPAL LLVRPAGALV EGLYCGTRDC YEVLGVSRSA
GKAEIARAYR QLARRYHPDR YRPQPGDEGP GRTPQSAEEA FLLVATAYET LKDEETRKDY
DYMLDHPEEY YSHYYHYYSR RLAPKVDVRV VILVSVCAIS VFQFFSWWNS YNKAISYLAT
VPKYRIQATE IAKQQGLLKK AKEKGKNKKS KEEIRDEEEN IIKNIIKSKI DIKGGYQKPQ
ICDLLLFQII LAPFHLCSYI VWYCRWIYNF NIKGKEYGEE ERLYIIRKSM KMSKSQFDSL
EDHQKETFLK RELWIKENYE VYKQEQEEEL KKKLANDPRW KRYRRWMKNE GPGRLTFVDD
