ID   DJC27_BOVIN             Reviewed;         273 AA.
AC   A5D7F5;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   12-JUN-2007, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=DnaJ homolog subfamily C member 27;
DE   AltName: Full=Rab and DnaJ domain-containing protein;
GN   Name=DNAJC27; Synonyms=RBJ;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal skin;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: GTPase which can activate the MEK/ERK pathway and induce cell
CC       transformation when overexpressed. May act as a nuclear scaffold for
CC       MAPK1, probably by association with MAPK1 nuclear export signal leading
CC       to enhanced ERK1/ERK2 signaling. {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SUBUNIT: Interacts directly with MAPK1 (wild-type and kinase-deficient
CC       forms). Interacts directly (in GTP-bound form) with MAP2K1 (wild-type
CC       and kinase-deficient forms). {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; BC140536; AAI40537.1; -; mRNA.
DR   RefSeq; NP_001091578.1; NM_001098109.1.
DR   AlphaFoldDB; A5D7F5; -.
DR   SMR; A5D7F5; -.
DR   STRING; 9913.ENSBTAP00000024278; -.
DR   PaxDb; A5D7F5; -.
DR   PRIDE; A5D7F5; -.
DR   Ensembl; ENSBTAT00000024278; ENSBTAP00000024278; ENSBTAG00000018239.
DR   GeneID; 540033; -.
DR   KEGG; bta:540033; -.
DR   CTD; 51277; -.
DR   VEuPathDB; HostDB:ENSBTAG00000018239; -.
DR   VGNC; VGNC:59212; DNAJC27.
DR   eggNOG; KOG0098; Eukaryota.
DR   GeneTree; ENSGT00940000157133; -.
DR   HOGENOM; CLU_041217_16_0_1; -.
DR   InParanoid; A5D7F5; -.
DR   OMA; MGNAETG; -.
DR   OrthoDB; 1288652at2759; -.
DR   TreeFam; TF328564; -.
DR   Proteomes; UP000009136; Chromosome 11.
DR   Bgee; ENSBTAG00000018239; Expressed in spermatid and 102 other tissues.
DR   ExpressionAtlas; A5D7F5; baseline and differential.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS51419; RAB; 1.
PE   2: Evidence at transcript level;
KW   GTP-binding; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..273
FT                   /note="DnaJ homolog subfamily C member 27"
FT                   /id="PRO_0000332974"
FT   DOMAIN          217..273
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   REGION          1..18
FT                   /note="Required for interaction with MAPK1"
FT                   /evidence="ECO:0000250|UniProtKB:Q8CFP6"
FT   BINDING         23..30
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         71..75
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         134..137
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   273 AA;  30759 MW;  466335916E28C3F2 CRC64;
     MEASMPKRKE PGKSLRIKVI SMGNAEVGKS CIIKRYCEKR FVSKYLATIG IDYGVTKVQV
     RDREIKVNIF DMAGDPFFYE VRNEFYKDTQ GVILVYDVGQ KDSFDALDTW LAEMKQDLGP
     HGNMENIVFA VCANKIDCTK HRCVDESEGR LWAESKGFLY FETSAQTGEG INEMFQTFYL
     SIVDLCENGG KRPNTNSSAS FTKEQADTIR RIRSSKDSWD MLGVKPGASR DEVNKAYRKL
     AVLLHPDKCV APGSEDAFKA VVNARTALLK NIK