ID   DJC27_PONAB             Reviewed;         273 AA.
AC   Q5RDE5;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 84.
DE   RecName: Full=DnaJ homolog subfamily C member 27;
DE   AltName: Full=Rab and DnaJ domain-containing protein;
GN   Name=DNAJC27; Synonyms=RBJ;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: GTPase which can activate the MEK/ERK pathway and induce cell
CC       transformation when overexpressed. May act as a nuclear scaffold for
CC       MAPK1, probably by association with MAPK1 nuclear export signal leading
CC       to enhanced ERK1/ERK2 signaling. {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SUBUNIT: Interacts directly with MAPK1 (wild-type and kinase-deficient
CC       forms). Interacts directly (in GTP-bound form) with MAP2K1 (wild-type
CC       and kinase-deficient forms). {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; CR857967; CAH90212.1; -; mRNA.
DR   RefSeq; NP_001125081.1; NM_001131609.1.
DR   AlphaFoldDB; Q5RDE5; -.
DR   SMR; Q5RDE5; -.
DR   STRING; 9601.ENSPPYP00000014064; -.
DR   GeneID; 100171963; -.
DR   KEGG; pon:100171963; -.
DR   CTD; 51277; -.
DR   eggNOG; KOG0098; Eukaryota.
DR   InParanoid; Q5RDE5; -.
DR   OrthoDB; 1288652at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS51419; RAB; 1.
PE   2: Evidence at transcript level;
KW   GTP-binding; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..273
FT                   /note="DnaJ homolog subfamily C member 27"
FT                   /id="PRO_0000332977"
FT   DOMAIN          217..273
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   REGION          1..18
FT                   /note="Required for interaction with MAPK1"
FT                   /evidence="ECO:0000250|UniProtKB:Q8CFP6"
FT   BINDING         23..30
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         71..75
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         134..137
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   273 AA;  30737 MW;  A0A3CE40FDD5602A CRC64;
     MEANMPKRKE PGRSLRIKVI SMGNAEVGKS CIIKRYCEKR FVSKYLATIG IDYGVTKVHV
     RDREIKVNIF DMAGHPFFYE VRNEFYKDTQ GVILVYDVGQ KDSFDALDAW LAEMKQELGP
     HGNMENIIFV VCANKIDCTK HRCVDESEGR LWAESKGFLY FETSAQTGEG INEMFQTFYI
     SIVDLCENGG KRPTTNSSAS FTKEQADAIR RIRNSKGSWD MLGVKPGASR DEVNKACRKL
     AVLLHPDKCV APGSEDAFKA VVNARTALLK NIK