ID   DJC27_RAT               Reviewed;         273 AA.
AC   Q6IML7;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=DnaJ homolog subfamily C member 27;
DE   AltName: Full=Rab and DnaJ domain-containing protein;
GN   Name=Dnajc27; Synonyms=Rbj;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-169.
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=14980719; DOI=10.1016/j.gene.2003.11.010;
RA   Nepomuceno-Silva J.L., de Melo L.D., Mendonca S.M., Paixao J.C.,
RA   Lopes U.G.;
RT   "RJLs: a new family of Ras-related GTP-binding proteins.";
RL   Gene 327:221-232(2004).
CC   -!- FUNCTION: GTPase which can activate the MEK/ERK pathway and induce cell
CC       transformation when overexpressed. May act as a nuclear scaffold for
CC       MAPK1, probably by association with MAPK1 nuclear export signal leading
CC       to enhanced ERK1/ERK2 signaling. {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SUBUNIT: Interacts directly with MAPK1 (wild-type and kinase-deficient
CC       forms). Interacts directly (in GTP-bound form) with MAP2K1 (wild-type
CC       and kinase-deficient forms). {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:Q8CFP6}.
CC   -!- SIMILARITY: Belongs to the small GTPase superfamily. Rab family.
CC       {ECO:0000305}.
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DR   EMBL; DY314277; -; NOT_ANNOTATED_CDS; mRNA.
DR   EMBL; BK001286; DAA01325.1; -; mRNA.
DR   RefSeq; NP_996727.2; NM_206845.2.
DR   AlphaFoldDB; Q6IML7; -.
DR   SMR; Q6IML7; -.
DR   STRING; 10116.ENSRNOP00000005317; -.
DR   PaxDb; Q6IML7; -.
DR   Ensembl; ENSRNOT00000005317; ENSRNOP00000005317; ENSRNOG00000003988.
DR   GeneID; 298859; -.
DR   KEGG; rno:298859; -.
DR   CTD; 51277; -.
DR   RGD; 1564414; Dnajc27.
DR   eggNOG; KOG0098; Eukaryota.
DR   GeneTree; ENSGT00940000157133; -.
DR   HOGENOM; CLU_041217_16_0_1; -.
DR   InParanoid; Q6IML7; -.
DR   OrthoDB; 1288652at2759; -.
DR   PRO; PR:Q6IML7; -.
DR   Proteomes; UP000002494; Chromosome 6.
DR   Bgee; ENSRNOG00000003988; Expressed in frontal cortex and 19 other tissues.
DR   ExpressionAtlas; Q6IML7; baseline and differential.
DR   Genevisible; Q6IML7; RN.
DR   GO; GO:0005634; C:nucleus; ISO:RGD.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0003924; F:GTPase activity; ISO:RGD.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; ISO:RGD.
DR   GO; GO:0043410; P:positive regulation of MAPK cascade; ISO:RGD.
DR   GO; GO:0071701; P:regulation of MAPK export from nucleus; ISO:RGD.
DR   CDD; cd06257; DnaJ; 1.
DR   Gene3D; 1.10.287.110; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   InterPro; IPR001623; DnaJ_domain.
DR   InterPro; IPR036869; J_dom_sf.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   InterPro; IPR001806; Small_GTPase.
DR   Pfam; PF00226; DnaJ; 1.
DR   Pfam; PF00071; Ras; 1.
DR   PRINTS; PR00625; JDOMAIN.
DR   SMART; SM00271; DnaJ; 1.
DR   SMART; SM00174; RHO; 1.
DR   SUPFAM; SSF46565; SSF46565; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR00231; small_GTP; 1.
DR   PROSITE; PS50076; DNAJ_2; 1.
DR   PROSITE; PS51419; RAB; 1.
PE   2: Evidence at transcript level;
KW   GTP-binding; Nucleotide-binding; Nucleus; Reference proteome.
FT   CHAIN           1..273
FT                   /note="DnaJ homolog subfamily C member 27"
FT                   /id="PRO_0000332978"
FT   DOMAIN          217..273
FT                   /note="J"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT   REGION          1..18
FT                   /note="Required for interaction with MAPK1"
FT                   /evidence="ECO:0000250|UniProtKB:Q8CFP6"
FT   BINDING         23..30
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         71..75
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
FT   BINDING         134..137
FT                   /ligand="GTP"
FT                   /ligand_id="ChEBI:CHEBI:37565"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   273 AA;  30949 MW;  C22DCCA9BB978970 CRC64;
     MESNVPKRKE PLKSLRIKVI SMGNAEVGKS CIIKRYCEKR FVSKYLATIG IDYGVTKVQV
     RDREIKVNIF DMAGHPFFFE VRNEFYKDTQ GVILVYDVGQ KDSFDALDSW LAEMKQELGP
     HGNMENIVFV VCANKIDCSK HRCIDESEGR LWAESRGFLY FETSAQTGEG INEMFQTFYM
     SIVDLCENGG KRPTANSSAS YTKEQADTIR RIRTSKDSWE MLGVRPGASR EEVNKAYRKL
     AVLLHPDKCV APGSEDAFKA VVNARTALLK NIK