DJC28_HUMAN
ID DJC28_HUMAN Reviewed; 388 AA.
AC Q9NX36; D3DSF2;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 19-SEP-2002, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=DnaJ homolog subfamily C member 28;
GN Name=DNAJC28; Synonyms=C21orf55, C21orf78;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12036298; DOI=10.1006/geno.2002.6782;
RA Gardiner K., Slavov D., Bechtel L., Davisson M.;
RT "Annotation of human chromosome 21 for relevance to Down syndrome: gene
RT structure and expression analysis.";
RL Genomics 79:833-843(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-347, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic kidney;
RX PubMed=17693683; DOI=10.1074/mcp.m700120-mcp200;
RA Tang L.-Y., Deng N., Wang L.-S., Dai J., Wang Z.-L., Jiang X.-S., Li S.-J.,
RA Li L., Sheng Q.-H., Wu D.-Q., Li L., Zeng R.;
RT "Quantitative phosphoproteome profiling of Wnt3a-mediated signaling
RT network: indicating the involvement of ribonucleoside-diphosphate reductase
RT M2 subunit phosphorylation at residue serine 20 in canonical Wnt signal
RT transduction.";
RL Mol. Cell. Proteomics 6:1952-1967(2007).
CC -!- FUNCTION: May have a role in protein folding or as a chaperone.
CC -!- TISSUE SPECIFICITY: Expressed in the fetal and adult brain, testis,
CC uterus, spleen and liver.
CC -!- DEVELOPMENTAL STAGE: Not expressed in 8 and 10 weeks old fetuses.
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DR EMBL; AF462153; AAL59618.2; -; mRNA.
DR EMBL; AK000468; BAA91185.1; -; mRNA.
DR EMBL; CH471079; EAX09830.1; -; Genomic_DNA.
DR EMBL; CH471079; EAX09831.1; -; Genomic_DNA.
DR CCDS; CCDS13626.1; -.
DR RefSeq; NP_001035282.1; NM_001040192.2.
DR RefSeq; NP_001307675.1; NM_001320746.2.
DR RefSeq; NP_060303.2; NM_017833.4.
DR AlphaFoldDB; Q9NX36; -.
DR SMR; Q9NX36; -.
DR BioGRID; 120283; 95.
DR IntAct; Q9NX36; 6.
DR MINT; Q9NX36; -.
DR STRING; 9606.ENSP00000479716; -.
DR iPTMnet; Q9NX36; -.
DR PhosphoSitePlus; Q9NX36; -.
DR BioMuta; DNAJC28; -.
DR DMDM; 23396536; -.
DR EPD; Q9NX36; -.
DR MassIVE; Q9NX36; -.
DR MaxQB; Q9NX36; -.
DR PaxDb; Q9NX36; -.
DR PeptideAtlas; Q9NX36; -.
DR PRIDE; Q9NX36; -.
DR ProteomicsDB; 83029; -.
DR Antibodypedia; 7335; 111 antibodies from 15 providers.
DR DNASU; 54943; -.
DR Ensembl; ENST00000314399.3; ENSP00000320303.3; ENSG00000177692.12.
DR Ensembl; ENST00000381947.4; ENSP00000371373.3; ENSG00000177692.12.
DR Ensembl; ENST00000402202.1; ENSP00000385777.1; ENSG00000177692.12.
DR Ensembl; ENST00000571432.5; ENSP00000461665.1; ENSG00000262911.5.
DR Ensembl; ENST00000572540.1; ENSP00000461737.1; ENSG00000262911.5.
DR Ensembl; ENST00000573306.1; ENSP00000461084.1; ENSG00000262911.5.
DR Ensembl; ENST00000615701.1; ENSP00000483670.1; ENSG00000262911.5.
DR Ensembl; ENST00000617313.4; ENSP00000479716.1; ENSG00000177692.12.
DR GeneID; 54943; -.
DR KEGG; hsa:54943; -.
DR MANE-Select; ENST00000381947.4; ENSP00000371373.3; NM_001040192.3; NP_001035282.1.
DR UCSC; uc002yrv.5; human.
DR CTD; 54943; -.
DR GeneCards; DNAJC28; -.
DR HGNC; HGNC:1297; DNAJC28.
DR HPA; ENSG00000177692; Tissue enhanced (testis).
DR neXtProt; NX_Q9NX36; -.
DR OpenTargets; ENSG00000177692; -.
DR PharmGKB; PA162383922; -.
DR VEuPathDB; HostDB:ENSG00000177692; -.
DR eggNOG; KOG0568; Eukaryota.
DR GeneTree; ENSGT00940000154065; -.
DR HOGENOM; CLU_040968_1_0_1; -.
DR InParanoid; Q9NX36; -.
DR OMA; TGFFNWM; -.
DR OrthoDB; 1394654at2759; -.
DR PhylomeDB; Q9NX36; -.
DR TreeFam; TF323730; -.
DR PathwayCommons; Q9NX36; -.
DR SignaLink; Q9NX36; -.
DR BioGRID-ORCS; 54943; 10 hits in 1065 CRISPR screens.
DR GeneWiki; C21orf55; -.
DR GenomeRNAi; 54943; -.
DR Pharos; Q9NX36; Tbio.
DR PRO; PR:Q9NX36; -.
DR Proteomes; UP000005640; Chromosome 21.
DR RNAct; Q9NX36; protein.
DR Bgee; ENSG00000177692; Expressed in islet of Langerhans and 96 other tissues.
DR Genevisible; Q9NX36; HS.
DR GO; GO:0017119; C:Golgi transport complex; IBA:GO_Central.
DR GO; GO:0007030; P:Golgi organization; IBA:GO_Central.
DR GO; GO:0048213; P:Golgi vesicle prefusion complex stabilization; IBA:GO_Central.
DR GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR018961; DnaJ_homolog_subfam-C_membr-28.
DR InterPro; IPR036869; J_dom_sf.
DR Pfam; PF00226; DnaJ; 1.
DR Pfam; PF09350; DUF1992; 1.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 1: Evidence at protein level;
KW Chaperone; Coiled coil; Phosphoprotein; Reference proteome.
FT CHAIN 1..388
FT /note="DnaJ homolog subfamily C member 28"
FT /id="PRO_0000071139"
FT DOMAIN 51..115
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT COILED 261..349
FT /evidence="ECO:0000255"
FT MOD_RES 347
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:17693683"
FT CONFLICT 316..388
FT /note="LIVPILTRQKVHFDAQKEIVRAQKIYETLIKTKEVTDRNPNNLDQGEGEKTP
FT EIKKGFLNWMNLWKFIKIRSF -> CSHPDQAKSPF (in Ref. 2; BAA91185)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 388 AA; 45806 MW; CB19D9FD060031D0 CRC64;
MNTMYVMMAQ ILRSHLIKAT VIPNRVKMLP YFGIIRNRMM STHKSKKKIR EYYRLLNVEE
GCSADEVRES FHKLAKQYHP DSGSNTADSA TFIRIEKAYR KVLSHVIEQT NASQSKGEEE
EDVEKFKYKT PQHRHYLSFE GIGFGTPTQR EKHYRQFRAD RAAEQVMEYQ KQKLQSQYFP
DSVIVKNIRQ SKQQKITQAI ERLVEDLIQE SMAKGDFDNL SGKGKPLKKF SDCSYIDPMT
HNLNRILIDN GYQPEWILKQ KEISDTIEQL REAILVSRKK LGNPMTPTEK KQWNHVCEQF
QENIRKLNKR INDFNLIVPI LTRQKVHFDA QKEIVRAQKI YETLIKTKEV TDRNPNNLDQ
GEGEKTPEIK KGFLNWMNLW KFIKIRSF
