DJP1_YEAST
ID DJP1_YEAST Reviewed; 432 AA.
AC P40564; D6VVT4; Q6Q5N6;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1995, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=DnaJ-like protein 1;
DE AltName: Full=Peroxisome assembly protein 22;
GN Name=DJP1; Synonyms=ICS1, PAS22; OrderedLocusNames=YIR004W; ORFNames=YIB4W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7762303; DOI=10.1002/yea.320110109;
RA Voss H., Tamames J., Teodoru C., Valencia A., Sensen C., Wiemann S.,
RA Schwager C., Zimmermann J., Sander C., Ansorge W.;
RT "Nucleotide sequence and analysis of the centromeric region of yeast
RT chromosome IX.";
RL Yeast 11:61-78(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169870;
RA Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL Nature 387:84-87(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [5]
RP FUNCTION.
RX PubMed=7507454; DOI=10.1093/genetics/135.3.731;
RA Elgersma Y., van den Berg M., Tabak H.F., Distel B.;
RT "An efficient positive selection procedure for the isolation of peroxisomal
RT import and peroxisome assembly mutants of Saccharomyces cerevisiae.";
RL Genetics 135:731-740(1993).
RN [6]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=9679141; DOI=10.1083/jcb.142.2.421;
RA Hettema E.H., Ruigrok C.C.M., Koerkamp M.G., van den Berg M., Tabak H.F.,
RA Distel B., Braakman I.;
RT "The cytosolic DnaJ-like protein djp1p is involved specifically in
RT peroxisomal protein import.";
RL J. Cell Biol. 142:421-434(1998).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [8]
RP INTERACTION WITH SLN1.
RX PubMed=15590828; DOI=10.1128/ec.3.6.1544-1556.2004;
RA Lu J.M.-Y., Deschenes R.J., Fassler J.S.;
RT "Role for the Ran binding protein, Mog1p, in Saccharomyces cerevisiae SLN1-
RT SKN7 signal transduction.";
RL Eukaryot. Cell 3:1544-1556(2004).
CC -!- FUNCTION: Required for peroxisomal protein import which maintains the
CC function of peroxisomes. {ECO:0000269|PubMed:7507454,
CC ECO:0000269|PubMed:9679141}.
CC -!- SUBUNIT: Interacts with SLN1. {ECO:0000269|PubMed:15590828}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:9679141}.
CC -!- MISCELLANEOUS: Present with 8970 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the DnaJ family. {ECO:0000305}.
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DR EMBL; X79743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; Z38062; CAA86206.1; -; Genomic_DNA.
DR EMBL; AY557886; AAS56212.1; -; Genomic_DNA.
DR EMBL; BK006942; DAA08550.1; -; Genomic_DNA.
DR PIR; S48438; S48438.
DR RefSeq; NP_012269.1; NM_001179526.1.
DR AlphaFoldDB; P40564; -.
DR SMR; P40564; -.
DR BioGRID; 34995; 55.
DR DIP; DIP-5923N; -.
DR IntAct; P40564; 1.
DR STRING; 4932.YIR004W; -.
DR TCDB; 3.A.30.1.1; the endoplasmic reticulum surface retrieval pathway (er-surf).
DR iPTMnet; P40564; -.
DR UCD-2DPAGE; P40564; -.
DR MaxQB; P40564; -.
DR PaxDb; P40564; -.
DR PRIDE; P40564; -.
DR EnsemblFungi; YIR004W_mRNA; YIR004W; YIR004W.
DR GeneID; 854820; -.
DR KEGG; sce:YIR004W; -.
DR SGD; S000001443; DJP1.
DR VEuPathDB; FungiDB:YIR004W; -.
DR eggNOG; KOG0691; Eukaryota.
DR GeneTree; ENSGT00940000176532; -.
DR HOGENOM; CLU_025145_3_1_1; -.
DR InParanoid; P40564; -.
DR OMA; TETDKGP; -.
DR BioCyc; YEAST:G3O-31425-MON; -.
DR PRO; PR:P40564; -.
DR Proteomes; UP000002311; Chromosome IX.
DR RNAct; P40564; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005933; C:cellular bud; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0005739; C:mitochondrion; IEA:GOC.
DR GO; GO:0051087; F:chaperone binding; ISS:SGD.
DR GO; GO:0016558; P:protein import into peroxisome matrix; IMP:SGD.
DR GO; GO:0045040; P:protein insertion into mitochondrial outer membrane; IMP:SGD.
DR CDD; cd06257; DnaJ; 1.
DR Gene3D; 1.10.287.110; -; 1.
DR InterPro; IPR001623; DnaJ_domain.
DR InterPro; IPR018253; DnaJ_domain_CS.
DR InterPro; IPR026894; DnaJ_X.
DR InterPro; IPR036869; J_dom_sf.
DR Pfam; PF00226; DnaJ; 1.
DR Pfam; PF14308; DnaJ-X; 1.
DR PRINTS; PR00625; JDOMAIN.
DR SMART; SM00271; DnaJ; 1.
DR SUPFAM; SSF46565; SSF46565; 1.
DR PROSITE; PS00636; DNAJ_1; 1.
DR PROSITE; PS50076; DNAJ_2; 1.
PE 1: Evidence at protein level;
KW Chaperone; Cytoplasm; Reference proteome.
FT CHAIN 1..432
FT /note="DnaJ-like protein 1"
FT /id="PRO_0000071141"
FT DOMAIN 4..73
FT /note="J"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00286"
FT REGION 117..187
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..134
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 135..152
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 325
FT /note="E -> G (in Ref. 4; AAS56212)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 432 AA; 48575 MW; 8127D6886BC78B96 CRC64;
MVVDTEYYDL LGVSTTASSI EIKKAYRKKS IQEHPDKNPN DPTATERFQA ISEAYQVLGD
DDLRAKYDKY GRKEAIPQGG FEDAAEQFSV IFGGDAFASY IGELMLLKNL QKTEELNAED
EAEKEKENVE TMEESPADGK TNGTTNAVDA ALGNTNEKDD KNKARTTSGN LTVHDGNKKN
EQVGAEAKKK KTKLEQFEEE QEVEKQKRVD QLSKTLIERL SILTESVYDD ACKDSFKKKF
EEEANLLKME SFGLDILHTI GDVYYEKAEI FLASQNLFGM GGIFHSMKAK GGVFMDTLRT
VSAAIDAQNT MKELEKMKEA STNNEPLFDK DGNEQIKPTT EELAQQEQLL MGKVLSAAWH
GSKYEITSTL RGVCKKVLED DSVSKKTLIR RAEAMKLLGE VFKKTFRTKV EQEEAQIFEE
LVAEATKKKR HT
