DJ_DROME
ID DJ_DROME Reviewed; 248 AA.
AC O01352; Q8INS3; Q9VI50;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Sperm-specific protein Don juan;
GN Name=dj; ORFNames=CG1980;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A).
RX PubMed=9232593; DOI=10.1016/s0925-4773(97)00031-2;
RA Santel A., Winhauer T., Bluemer N., Renkawitz-Pohl R.;
RT "The Drosophila don juan (dj) gene encodes a novel sperm specific protein
RT component characterized by an unusual domain of a repetitive amino acid
RT motif.";
RL Mech. Dev. 64:19-30(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE (ISOFORM A).
RC STRAIN=Berkeley;
RA Celniker S.E., George R.A., Galle R., Svirskas R.R., Hoskins R.A.,
RA Agbayani A., Arcaina T.T., Baxter E., Blazej R.G., Chavez C., Chew M.,
RA Doyle C.M., Farfan D.E., Flanagan J., Houston K.A., Hummasti S.R.,
RA Karra K., Kearney L., Kim S.H., Lee B., Lomotan M.A., Mak J., Mazda P.,
RA Mok M.S., Moshrefi A.R., Moshrefi M., Nixon K., Pacleb J.M., Park S.,
RA Pfeiffer B., Punch E., Snir E., Twomey B., Wan K.H., Whitelaw K.R., Yee A.,
RA Zhang R., Zieran L.L., Kimmel B.E.;
RT "Sequencing of ANT-C antennapedia complex.";
RL Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=9788872; DOI=10.1242/jcs.111.22.3299;
RA Santel A., Bluemer N., Kaempfer M., Renkawitz-Pohl R.;
RT "Flagellar mitochondrial association of the male-specific Don Juan protein
RT in Drosophila spermatozoa.";
RL J. Cell Sci. 111:3299-3309(1998).
CC -!- FUNCTION: May be involved in the final steps of mitochondrial
CC differentiation within the flagellum.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9788872}.
CC Mitochondrion {ECO:0000269|PubMed:9788872}. Note=Predominantly nuclear.
CC Some fraction is associated with mitochondria.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=O01352-1; Sequence=Displayed;
CC Name=B;
CC IsoId=O01352-2; Sequence=VSP_009364;
CC -!- TISSUE SPECIFICITY: Expression limited to post-meiotic male germ cells.
CC Expressed in elongated spermatids during individualization and in
CC finally elongated nuclei of spermatids. After completion of nuclear
CC shaping it is no longer expressed in the sperm heads with the onset of
CC individualization. {ECO:0000269|PubMed:9788872}.
CC -!- DEVELOPMENTAL STAGE: Expressed post-meiotically.
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DR EMBL; U90537; AAC05722.1; -; mRNA.
DR EMBL; AC004266; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AE014297; AAF54078.3; -; Genomic_DNA.
DR EMBL; AE014297; AAN13355.2; -; Genomic_DNA.
DR RefSeq; NP_477220.3; NM_057872.5. [O01352-2]
DR RefSeq; NP_731118.3; NM_169163.4. [O01352-1]
DR AlphaFoldDB; O01352; -.
DR SMR; O01352; -.
DR IntAct; O01352; 3.
DR MINT; O01352; -.
DR STRING; 7227.FBpp0088683; -.
DR PaxDb; O01352; -.
DR PRIDE; O01352; -.
DR EnsemblMetazoa; FBtr0089741; FBpp0088682; FBgn0019828. [O01352-2]
DR EnsemblMetazoa; FBtr0089742; FBpp0088683; FBgn0019828. [O01352-1]
DR GeneID; 40838; -.
DR KEGG; dme:Dmel_CG1980; -.
DR CTD; 40838; -.
DR FlyBase; FBgn0019828; dj.
DR VEuPathDB; VectorBase:FBgn0019828; -.
DR eggNOG; ENOG502TBEV; Eukaryota.
DR GeneTree; ENSGT00940000169927; -.
DR PhylomeDB; O01352; -.
DR BioGRID-ORCS; 40838; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40838; -.
DR PRO; PR:O01352; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0019828; Expressed in seminal fluid secreting gland and 10 other tissues.
DR ExpressionAtlas; O01352; baseline and differential.
DR Genevisible; O01352; DM.
DR GO; GO:0005930; C:axoneme; IDA:FlyBase.
DR GO; GO:0016007; C:mitochondrial derivative; IDA:FlyBase.
DR GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR GO; GO:0031514; C:motile cilium; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0007291; P:sperm individualization; IEP:FlyBase.
DR GO; GO:0007283; P:spermatogenesis; TAS:FlyBase.
PE 2: Evidence at transcript level;
KW Alternative splicing; Coiled coil; Mitochondrion; Nucleus;
KW Reference proteome; Repeat.
FT CHAIN 1..248
FT /note="Sperm-specific protein Don juan"
FT /id="PRO_0000079924"
FT REPEAT 147..152
FT /note="1"
FT REPEAT 153..158
FT /note="2"
FT REPEAT 159..164
FT /note="3"
FT REPEAT 165..170
FT /note="4"
FT REPEAT 171..176
FT /note="5"
FT REPEAT 177..182
FT /note="6"
FT REPEAT 183..188
FT /note="7"
FT REPEAT 189..194
FT /note="8"
FT REGION 146..200
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 147..194
FT /note="8 X 6 AA tandem repeat of D-P-C-K-K-K"
FT COILED 82..147
FT /evidence="ECO:0000255"
FT COILED 197..244
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..39
FT /note="MFKRTALILRRCFQPTFIRPHHINVLENFKEADDLPNQG -> MSLRTLRKG
FT (in isoform B)"
FT /evidence="ECO:0000305"
FT /id="VSP_009364"
FT CONFLICT 27
FT /note="E -> D (in Ref. 1; AAC05722)"
FT /evidence="ECO:0000305"
FT CONFLICT 39
FT /note="G -> R (in Ref. 1; AAC05722)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 248 AA; 29127 MW; 5294BF0FF60E7361 CRC64;
MFKRTALILR RCFQPTFIRP HHINVLENFK EADDLPNQGQ AKFVDVSIHD PQHIRSALVS
PMQRKFLQDL EQQQTVRIKW FKEGNQDELE NMKNECRRLA LEIIMAAKGG DIKKACKELA
EKEKCKQIEL KKKCKELEKK TKCAKKDPCK KKDPCKKKDP CKKKDPCKKK DPCKKKDPCK
KKDPCKKKDP CKKKGGDLKK KCKKLAEKEK CKKLAKKEKM KKLQKKCKKM AQKEKCKKMA
KKDKCKKK
