DKC1_DICDI
ID DKC1_DICDI Reviewed; 540 AA.
AC Q54T81;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 115.
DE RecName: Full=Probable H/ACA ribonucleoprotein complex subunit 4;
DE EC=5.4.99.-;
GN Name=nola4; Synonyms=dkc1; ORFNames=DDB_G0281933;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=16512674; DOI=10.1021/pr050350q;
RA Reinders Y., Schulz I., Graef R., Sickmann A.;
RT "Identification of novel centrosomal proteins in Dictyostelium discoideum
RT by comparative proteomic approaches.";
RL J. Proteome Res. 5:589-598(2006).
CC -!- FUNCTION: Plays a central role in ribosomal RNA processing. Probable
CC catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA
CC snoRNP) complex, which catalyzes pseudouridylation of rRNA. This
CC involves the isomerization of uridine such that the ribose is
CC subsequently attached to C5, instead of the normal N1. Pseudouridine
CC ('psi') residues may serve to stabilize the conformation of rRNAs (By
CC similarity). {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a uridine in RNA = a pseudouridine in RNA;
CC Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC -!- SUBUNIT: Component of the small nucleolar ribonucleoprotein particles
CC containing H/ACA-type snoRNAs (H/ACA snoRNPs). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000043; EAL66526.1; -; Genomic_DNA.
DR RefSeq; XP_640508.1; XM_635416.1.
DR AlphaFoldDB; Q54T81; -.
DR SMR; Q54T81; -.
DR IntAct; Q54T81; 1.
DR STRING; 44689.DDB0233679; -.
DR PaxDb; Q54T81; -.
DR EnsemblProtists; EAL66526; EAL66526; DDB_G0281933.
DR GeneID; 8623323; -.
DR KEGG; ddi:DDB_G0281933; -.
DR dictyBase; DDB_G0281933; nola4.
DR eggNOG; KOG2529; Eukaryota.
DR HOGENOM; CLU_032087_3_2_1; -.
DR InParanoid; Q54T81; -.
DR OMA; GPFKEDE; -.
DR PhylomeDB; Q54T81; -.
DR Reactome; R-DDI-171319; Telomere Extension By Telomerase.
DR PRO; PR:Q54T81; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0031429; C:box H/ACA snoRNP complex; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; IDA:dictyBase.
DR GO; GO:0005697; C:telomerase holoenzyme complex; ISS:dictyBase.
DR GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; ISS:dictyBase.
DR GO; GO:0003720; F:telomerase activity; ISS:dictyBase.
DR GO; GO:0000495; P:box H/ACA RNA 3'-end processing; IBA:GO_Central.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IMP:dictyBase.
DR GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0046689; P:response to mercury ion; IDA:dictyBase.
DR GO; GO:0006979; P:response to oxidative stress; IMP:dictyBase.
DR GO; GO:0031118; P:rRNA pseudouridine synthesis; IBA:GO_Central.
DR GO; GO:0031120; P:snRNA pseudouridine synthesis; IBA:GO_Central.
DR Gene3D; 2.30.130.10; -; 1.
DR InterPro; IPR012960; Dyskerin-like.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR002501; PsdUridine_synth_N.
DR InterPro; IPR002478; PUA.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR InterPro; IPR032819; TruB_C.
DR InterPro; IPR004521; Uncharacterised_CHP00451.
DR PANTHER; PTHR23127; PTHR23127; 1.
DR Pfam; PF08068; DKCLD; 1.
DR Pfam; PF01472; PUA; 1.
DR Pfam; PF16198; TruB_C_2; 1.
DR Pfam; PF01509; TruB_N; 1.
DR SMART; SM01136; DKCLD; 1.
DR SMART; SM00359; PUA; 1.
DR SUPFAM; SSF55120; SSF55120; 1.
DR SUPFAM; SSF88697; SSF88697; 1.
DR TIGRFAMs; TIGR00425; CBF5; 1.
DR TIGRFAMs; TIGR00451; unchar_dom_2; 1.
DR PROSITE; PS50890; PUA; 1.
PE 1: Evidence at protein level;
KW Coiled coil; Isomerase; Nucleus; Reference proteome; Ribonucleoprotein;
KW Ribosome biogenesis; RNA-binding; rRNA processing.
FT CHAIN 1..540
FT /note="Probable H/ACA ribonucleoprotein complex subunit 4"
FT /id="PRO_0000331202"
FT DOMAIN 280..355
FT /note="PUA"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 414..540
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 448..494
FT /evidence="ECO:0000255"
FT COMPBIAS 1..21
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 436..531
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 109
FT /note="Nucleophile"
FT /evidence="ECO:0000250"
SQ SEQUENCE 540 AA; 60804 MW; 9418744A362617C9 CRC64;
MTTDKKSKSK SSEKSTQEVE QVIKPEKTPI LDTSKWPLLL KNYDQLSVRT GHYTPIPNGH
SPLKRPIKEY VKYGIINLDK PSNPSSHEVV AWIRTILRVT KTGHSGTLDP KVTGCLIVCI
ERATRLVKSQ QGAGKEYIGI VRLHGAIEGT TELSKAVDTL TGALFQRPPQ KSAVKKRLRV
RTIHNSKLLE FDPERNLGLI WVDCEAGTYI RTLCVHIGLL MGVGGHMQEL RRVRSGIMSE
KKGQVTMHDV KDAQYEYDNS KDESYLRRVI QPLEAILTNY KRIVVKDSAI NAICYGAKLM
IPGLLRYEQD IESNEEVVLI TTKGEAIAIG IAQMTTASMA TCDHGVVATI KRVIMDRDVY
PVRWGLGPKA KVKKEMIKDG KLDKFGKPNE KTPSDWTNTY TYYTGQVATT TTTSPVESMN
VDTPKKATAS VAVKAEVESS EDEKPVPKKE KKDKKEKKKD SSDDESEEEK SSKKDKKEKK
EKKEKKEKKS SKDDSDDESS KKEKKDKKRK KSSDKDSDSE KESSDKKDKK DKKEKKKSKN
