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DKC1_DROME
ID   DKC1_DROME              Reviewed;         508 AA.
AC   O44081; A8E745; Q0GLB4; Q0MVP1; Q8T408; Q9UAN8; Q9V3Z5;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 171.
DE   RecName: Full=H/ACA ribonucleoprotein complex subunit 4;
DE            EC=5.4.99.-;
DE   AltName: Full=Nucleolar protein AT band 60B;
DE   AltName: Full=Protein minifly;
GN   Name=Nop60B; Synonyms=mfl; ORFNames=CG3333;
OS   Drosophila melanogaster (Fruit fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC   Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=7227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=9829824; DOI=10.1007/s004380050866;
RA   Phillips B., Billin A.N., Cadwell C., Buchholz R., Erickson C.,
RA   Merriam J.R., Carbon J., Poole S.J.;
RT   "The Nop60B gene of Drosophila encodes an essential nucleolar protein that
RT   functions in yeast.";
RL   Mol. Gen. Genet. 260:20-29(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM A), FUNCTION, AND
RP   SUBCELLULAR LOCATION.
RX   PubMed=10087258; DOI=10.1083/jcb.144.6.1123;
RA   Giordano E., Peluso I., Senger S., Furia M.;
RT   "minifly, a Drosophila gene required for ribosome biogenesis.";
RL   J. Cell Biol. 144:1123-1133(1999).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND C), AND DEVELOPMENTAL STAGE.
RX   PubMed=17328797; DOI=10.1186/1471-2199-8-15;
RA   Riccardo S., Tortoriello G., Giordano E., Turano M., Furia M.;
RT   "The coding/non-coding overlapping architecture of the gene encoding the
RT   Drosophila pseudouridine synthase.";
RL   BMC Mol. Biol. 8:15-15(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Berkeley;
RX   PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA   Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA   Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA   George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA   Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA   Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA   Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA   An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA   Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA   Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA   Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA   Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA   Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA   Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA   Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA   Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA   Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA   Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA   Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA   Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA   Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA   Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA   McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA   Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA   Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA   Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA   Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA   Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA   Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA   Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA   Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA   Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA   Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA   Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA   Venter J.C.;
RT   "The genome sequence of Drosophila melanogaster.";
RL   Science 287:2185-2195(2000).
RN   [5]
RP   GENOME REANNOTATION.
RC   STRAIN=Berkeley;
RX   PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA   Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA   Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA   Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA   Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA   Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA   Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT   "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT   review.";
RL   Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-468 (ISOFORM A).
RC   STRAIN=Berkeley; TISSUE=Testis;
RX   PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA   Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA   Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA   Celniker S.E.;
RT   "A Drosophila full-length cDNA resource.";
RL   Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-468 (ISOFORM A).
RC   STRAIN=Berkeley; TISSUE=Embryo;
RA   Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA   Celniker S.E.;
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN   [8]
RP   FUNCTION.
RX   PubMed=12645924; DOI=10.1016/s0012-1606(02)00013-1;
RA   Kauffman T., Tran J., DiNardo S.;
RT   "Mutations in Nop60B, the Drosophila homolog of human dyskeratosis
RT   congenita 1, affect the maintenance of the germ-line stem cell lineage
RT   during spermatogenesis.";
RL   Dev. Biol. 253:189-199(2003).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-442; THR-443; SER-444;
RP   SER-445; THR-449; SER-455 AND THR-458, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   TISSUE=Embryo;
RX   PubMed=18327897; DOI=10.1021/pr700696a;
RA   Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT   "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL   J. Proteome Res. 7:1675-1682(2008).
CC   -!- FUNCTION: Plays a central role in ribosomal RNA processing. Probable
CC       catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA
CC       snoRNP) complex, which catalyzes pseudouridylation of rRNA. This
CC       involves the isomerization of uridine such that the ribose is
CC       subsequently attached to C5, instead of the normal N1. Pseudouridine
CC       ('psi') residues may serve to stabilize the conformation of rRNAs.
CC       Required for maintenance of the germline stem cell lineage during
CC       spermatogenesis. {ECO:0000269|PubMed:10087258,
CC       ECO:0000269|PubMed:12645924, ECO:0000269|PubMed:9829824}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a uridine in RNA = a pseudouridine in RNA;
CC         Xref=Rhea:RHEA:48348, Rhea:RHEA-COMP:12068, Rhea:RHEA-COMP:12069,
CC         ChEBI:CHEBI:65314, ChEBI:CHEBI:65315;
CC   -!- SUBUNIT: Component of the small nucleolar ribonucleoprotein particle
CC       containing H/ACA-type snoRNAs (H/ACA snoRNPs). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:10087258,
CC       ECO:0000269|PubMed:9829824}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=A; Synonyms=B;
CC         IsoId=O44081-1; Sequence=Displayed;
CC       Name=C; Synonyms=alpha;
CC         IsoId=O44081-2; Sequence=VSP_036583, VSP_036584;
CC   -!- DEVELOPMENTAL STAGE: Isoform C is expressed both maternally and
CC       zygotically. Isoform A is expressed at the same level in adult females
CC       but at a much lower level in ovaries. {ECO:0000269|PubMed:17328797}.
CC   -!- SIMILARITY: Belongs to the pseudouridine synthase TruB family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABV82369.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AF017230; AAC97117.1; -; mRNA.
DR   EMBL; AF089837; AAD16092.1; -; mRNA.
DR   EMBL; AF097634; AAD19897.1; -; Genomic_DNA.
DR   EMBL; DQ841550; ABH08965.1; -; mRNA.
DR   EMBL; DQ857345; ABI17547.1; -; mRNA.
DR   EMBL; DQ857346; ABI17548.1; -; mRNA.
DR   EMBL; AE013599; AAF47178.1; -; Genomic_DNA.
DR   EMBL; AE013599; AAX52682.1; -; Genomic_DNA.
DR   EMBL; AY089408; AAL90146.1; -; mRNA.
DR   EMBL; BT030987; ABV82369.1; ALT_INIT; mRNA.
DR   RefSeq; NP_001014547.1; NM_001014547.4. [O44081-1]
DR   RefSeq; NP_001163286.1; NM_001169815.3. [O44081-1]
DR   RefSeq; NP_001163289.1; NM_001169818.3. [O44081-1]
DR   RefSeq; NP_525120.1; NM_080381.5. [O44081-1]
DR   AlphaFoldDB; O44081; -.
DR   SMR; O44081; -.
DR   BioGRID; 63449; 11.
DR   DIP; DIP-20128N; -.
DR   IntAct; O44081; 3.
DR   STRING; 7227.FBpp0099583; -.
DR   iPTMnet; O44081; -.
DR   PaxDb; O44081; -.
DR   EnsemblMetazoa; FBtr0072258; FBpp0072166; FBgn0259937. [O44081-1]
DR   EnsemblMetazoa; FBtr0100213; FBpp0099583; FBgn0259937. [O44081-1]
DR   EnsemblMetazoa; FBtr0300227; FBpp0289464; FBgn0259937. [O44081-1]
DR   EnsemblMetazoa; FBtr0300861; FBpp0290083; FBgn0259937. [O44081-1]
DR   GeneID; 37873; -.
DR   KEGG; dme:Dmel_CG3333; -.
DR   UCSC; CG3333-RB; d. melanogaster.
DR   CTD; 37873; -.
DR   FlyBase; FBgn0259937; Nop60B.
DR   VEuPathDB; VectorBase:FBgn0259937; -.
DR   eggNOG; KOG2529; Eukaryota.
DR   GeneTree; ENSGT00510000047092; -.
DR   HOGENOM; CLU_032087_3_2_1; -.
DR   InParanoid; O44081; -.
DR   OMA; GPFKEDE; -.
DR   PhylomeDB; O44081; -.
DR   Reactome; R-DME-171319; Telomere Extension By Telomerase.
DR   SignaLink; O44081; -.
DR   BioGRID-ORCS; 37873; 0 hits in 1 CRISPR screen.
DR   ChiTaRS; Nop60B; fly.
DR   GenomeRNAi; 37873; -.
DR   PRO; PR:O44081; -.
DR   Proteomes; UP000000803; Chromosome 2R.
DR   Bgee; FBgn0259937; Expressed in ovary and 14 other tissues.
DR   ExpressionAtlas; O44081; baseline and differential.
DR   Genevisible; O44081; DM.
DR   GO; GO:0031429; C:box H/ACA snoRNP complex; IBA:GO_Central.
DR   GO; GO:0005730; C:nucleolus; IDA:FlyBase.
DR   GO; GO:0009982; F:pseudouridine synthase activity; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000495; P:box H/ACA RNA 3'-end processing; IBA:GO_Central.
DR   GO; GO:0007281; P:germ cell development; IMP:FlyBase.
DR   GO; GO:1990481; P:mRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0001522; P:pseudouridine synthesis; IMP:FlyBase.
DR   GO; GO:0042254; P:ribosome biogenesis; IMP:FlyBase.
DR   GO; GO:0006364; P:rRNA processing; IMP:FlyBase.
DR   GO; GO:0031118; P:rRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0031120; P:snRNA pseudouridine synthesis; IBA:GO_Central.
DR   GO; GO:0035220; P:wing disc development; IMP:FlyBase.
DR   Gene3D; 2.30.130.10; -; 1.
DR   InterPro; IPR012960; Dyskerin-like.
DR   InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR   InterPro; IPR002501; PsdUridine_synth_N.
DR   InterPro; IPR002478; PUA.
DR   InterPro; IPR015947; PUA-like_sf.
DR   InterPro; IPR036974; PUA_sf.
DR   InterPro; IPR004802; tRNA_PsdUridine_synth_B_fam.
DR   InterPro; IPR032819; TruB_C.
DR   PANTHER; PTHR23127; PTHR23127; 1.
DR   Pfam; PF08068; DKCLD; 1.
DR   Pfam; PF01472; PUA; 1.
DR   Pfam; PF16198; TruB_C_2; 1.
DR   Pfam; PF01509; TruB_N; 1.
DR   SMART; SM01136; DKCLD; 1.
DR   SMART; SM00359; PUA; 1.
DR   SUPFAM; SSF55120; SSF55120; 1.
DR   SUPFAM; SSF88697; SSF88697; 1.
DR   TIGRFAMs; TIGR00425; CBF5; 1.
DR   PROSITE; PS50890; PUA; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Isomerase; Nucleus; Phosphoprotein;
KW   Reference proteome; Ribonucleoprotein; Ribosome biogenesis; RNA-binding;
KW   rRNA processing.
FT   CHAIN           1..508
FT                   /note="H/ACA ribonucleoprotein complex subunit 4"
FT                   /id="PRO_0000121989"
FT   DOMAIN          294..369
FT                   /note="PUA"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00161"
FT   REGION          1..29
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          423..508
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..24
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        424..451
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        484..508
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        123
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   MOD_RES         442
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         443
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         444
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         445
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         449
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         455
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   MOD_RES         458
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000269|PubMed:18327897"
FT   VAR_SEQ         212..253
FT                   /note="GVFWVSCEAGSYIRTMCVHLGLVLGVGGQMLELRRVRSGIQS -> DLLIRS
FT                   CNIWTITLIKEISSKIFLKIIECHRNLKSSHDTLLR (in isoform C)"
FT                   /evidence="ECO:0000303|PubMed:17328797"
FT                   /id="VSP_036583"
FT   VAR_SEQ         254..508
FT                   /note="Missing (in isoform C)"
FT                   /evidence="ECO:0000303|PubMed:17328797"
FT                   /id="VSP_036584"
FT   CONFLICT        69
FT                   /note="P -> R (in Ref. 2; AAD19897)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   508 AA;  56831 MW;  3CAE3F91C84E0A94 CRC64;
     MADVEVRKEK KKKKIKEEPL DGDDIGTLQK QGNFQIKPSS KIAELDTSQW PLLLKNFDKL
     NIRSNHYTPL AHGSSPLNRD IKEYMKTGFI NLDKPSNPSS HEVVAWIKKI LKVEKTGHSG
     TLDPKVTGCL IVCIDRATRL VKSQQSAGKE YVAIFKLHGA VESVAKVRQG LEKLRGALFQ
     RPPLISAVKR QLRVRTVYDS KLLDYDETRN MGVFWVSCEA GSYIRTMCVH LGLVLGVGGQ
     MLELRRVRSG IQSERDGMVT MHDVLDAMWL YENHKDESML RRVIKPLEGL LVNHKRIIMK
     DSSVNAVCYG AKITLPGVLR YEDGIEIDQE IVICTTKGEA ICLAIALMTT ATMASCDHGV
     VAKIKRVIME RDTYPRKWGL GPKASAKKAL IAAGKLDKFG RPNENTPKEW LTGYVDYNAK
     KPAAQEVSPT NGSSEPSKRK LSTSSVEETA AAAVSEETPS KDKKKKKKKH KGDEEAPEAA
     EEEAEPVEKE KKKKKKKDKD RDRDEAQE
 
 
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