DKKL1_HUMAN
ID DKKL1_HUMAN Reviewed; 242 AA.
AC Q9UK85;
DT 21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Dickkopf-like protein 1 {ECO:0000305};
DE AltName: Full=Cancer/testis antigen 34;
DE Short=CT34;
DE AltName: Full=Protein soggy-1;
DE Short=SGY-1 {ECO:0000303|PubMed:10570958};
DE Flags: Precursor;
GN Name=DKKL1 {ECO:0000312|HGNC:HGNC:16528};
GN Synonyms=SGY1 {ECO:0000312|HGNC:HGNC:16528}; ORFNames=UNQ735/PRO1429;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10570958; DOI=10.1016/s0378-1119(99)00365-0;
RA Krupnik V.E., Sharp J.D., Jiang C., Robison K., Chickering T.W.,
RA Amaravadi L., Brown D.E., Guyot D., Mays G., Leiby K., Chang B., Duong T.,
RA Goodearl A.D.J., Gearing D.P., Sokol S.Y., McCarthy S.A.;
RT "Functional and structural diversity of the human Dickkopf gene family.";
RL Gene 238:301-313(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Tate G.;
RT "Genomic organization of the human and mouse Soggy.";
RL Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=22817830; DOI=10.1186/1477-7827-10-51;
RA Yan Q., Wu X., Chen C., Diao R., Lai Y., Huang J., Chen J., Yu Z., Gui Y.,
RA Tang A., Cai Z.;
RT "Developmental expression and function of DKKL1/Dkkl1 in humans and mice.";
RL Reprod. Biol. Endocrinol. 10:51-51(2012).
CC -!- FUNCTION: Involved in fertilization by facilitating sperm penetration
CC of the zona pellucida. May promote spermatocyte apoptosis, thereby
CC limiting sperm production. In adults, may reduce testosterone synthesis
CC in Leydig cells. Is not essential either for development or fertility.
CC {ECO:0000250|UniProtKB:Q9QZL9}.
CC -!- SUBUNIT: Interacts with SLXL1; Co-localize in seminiferous tubules.
CC Interacts with SLY. {ECO:0000250|UniProtKB:Q9QZL9}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9QZL9}.
CC Cytoplasmic vesicle, secretory vesicle, acrosome
CC {ECO:0000250|UniProtKB:Q9QZL9}. Note=Localized specifically to the
CC crescent shaped acrosome at the apex of the sperm head.
CC {ECO:0000250|UniProtKB:Q9QZL9}.
CC -!- TISSUE SPECIFICITY: More highly expressed in adult testis than in fetal
CC testis. Exclusively expressed in the testis (at protein level). Intense
CC expression in stages II, III and IV of spermatogenesis, whereas
CC expression is lower in stage I. {ECO:0000269|PubMed:22817830}.
CC -!- INDUCTION: Abnormal expression in testes of patients with male
CC infertility. {ECO:0000269|PubMed:22817830}.
CC -!- DOMAIN: Contains a N-terminal domain similar to that of the N-terminal
CC section of DKK3.
CC -!- PTM: N-glycosylated during spermatogenesis. Not N-glycosylated in
CC mature sperm. {ECO:0000250|UniProtKB:Q9QZL9}.
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DR EMBL; AF177398; AAF02678.1; -; mRNA.
DR EMBL; AB047818; BAB12400.1; -; Genomic_DNA.
DR EMBL; AY358922; AAQ89281.1; -; mRNA.
DR EMBL; BC030581; AAH30581.1; -; mRNA.
DR CCDS; CCDS12762.1; -.
DR RefSeq; NP_001184230.1; NM_001197301.1.
DR RefSeq; NP_001184231.1; NM_001197302.1.
DR RefSeq; NP_055234.1; NM_014419.3.
DR AlphaFoldDB; Q9UK85; -.
DR BioGRID; 118011; 117.
DR IntAct; Q9UK85; 78.
DR STRING; 9606.ENSP00000221498; -.
DR GlyGen; Q9UK85; 2 sites.
DR iPTMnet; Q9UK85; -.
DR PhosphoSitePlus; Q9UK85; -.
DR BioMuta; DKKL1; -.
DR DMDM; 13124528; -.
DR MassIVE; Q9UK85; -.
DR PaxDb; Q9UK85; -.
DR PeptideAtlas; Q9UK85; -.
DR PRIDE; Q9UK85; -.
DR ProteomicsDB; 84740; -.
DR Antibodypedia; 31990; 270 antibodies from 27 providers.
DR DNASU; 27120; -.
DR Ensembl; ENST00000221498.7; ENSP00000221498.1; ENSG00000104901.7.
DR GeneID; 27120; -.
DR KEGG; hsa:27120; -.
DR MANE-Select; ENST00000221498.7; ENSP00000221498.1; NM_014419.4; NP_055234.1.
DR UCSC; uc002pnk.4; human.
DR CTD; 27120; -.
DR DisGeNET; 27120; -.
DR GeneCards; DKKL1; -.
DR HGNC; HGNC:16528; DKKL1.
DR HPA; ENSG00000104901; Tissue enriched (testis).
DR MIM; 605418; gene.
DR neXtProt; NX_Q9UK85; -.
DR OpenTargets; ENSG00000104901; -.
DR PharmGKB; PA134974559; -.
DR VEuPathDB; HostDB:ENSG00000104901; -.
DR eggNOG; ENOG502SZ2Z; Eukaryota.
DR GeneTree; ENSGT00390000014026; -.
DR InParanoid; Q9UK85; -.
DR OMA; RRSHQDT; -.
DR OrthoDB; 1323997at2759; -.
DR PhylomeDB; Q9UK85; -.
DR TreeFam; TF337340; -.
DR PathwayCommons; Q9UK85; -.
DR SignaLink; Q9UK85; -.
DR BioGRID-ORCS; 27120; 10 hits in 1066 CRISPR screens.
DR GenomeRNAi; 27120; -.
DR Pharos; Q9UK85; Tbio.
DR PRO; PR:Q9UK85; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9UK85; protein.
DR Bgee; ENSG00000104901; Expressed in left testis and 126 other tissues.
DR ExpressionAtlas; Q9UK85; baseline and differential.
DR Genevisible; Q9UK85; HS.
DR GO; GO:0001669; C:acrosomal vesicle; ISS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0039706; F:co-receptor binding; IBA:GO_Central.
DR GO; GO:0048019; F:receptor antagonist activity; IBA:GO_Central.
DR GO; GO:0009653; P:anatomical structure morphogenesis; TAS:ProtInc.
DR GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IBA:GO_Central.
DR GO; GO:2000225; P:negative regulation of testosterone biosynthetic process; ISS:UniProtKB.
DR GO; GO:0007341; P:penetration of zona pellucida; ISS:UniProtKB.
DR GO; GO:0043065; P:positive regulation of apoptotic process; ISS:UniProtKB.
DR GO; GO:0045600; P:positive regulation of fat cell differentiation; IEA:Ensembl.
DR InterPro; IPR039863; DKK-like.
DR PANTHER; PTHR12113; PTHR12113; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Glycoprotein; Reference proteome; Secreted; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..242
FT /note="Dickkopf-like protein 1"
FT /id="PRO_0000007228"
FT CARBOHYD 97
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 112
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VARIANT 24
FT /note="L -> R (in dbSNP:rs2303757)"
FT /id="VAR_053061"
FT VARIANT 29
FT /note="A -> T (in dbSNP:rs919364)"
FT /id="VAR_024432"
FT VARIANT 104
FT /note="L -> I (in dbSNP:rs35693137)"
FT /id="VAR_053062"
FT VARIANT 109
FT /note="M -> R (in dbSNP:rs2303759)"
FT /id="VAR_021967"
FT VARIANT 187
FT /note="G -> S (in dbSNP:rs1054770)"
FT /id="VAR_053063"
FT VARIANT 214
FT /note="E -> K (in dbSNP:rs2288481)"
FT /id="VAR_021968"
SQ SEQUENCE 242 AA; 27007 MW; 8811A4460CB5389E CRC64;
MGEASPPAPA RRHLLVLLLL LSTLVIPSAA APIHDADAQE SSLGLTGLQS LLQGFSRLFL
KGNLLRGIDS LFSAPMDFRG LPGNYHKEEN QEHQLGNNTL SSHLQIDKMT DNKTGEVLIS
ENVVASIQPA EGSFEGDLKV PRMEEKEALV PIQKATDSFH TELHPRVAFW IIKLPRRRSH
QDALEGGHWL SEKRHRLQAI RDGLRKGTHK DVLEEGTESS SHSRLSPRKT HLLYILRPSR
QL
