ID   DKSA_BUCAI              Reviewed;         151 AA.
AC   P57294;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=RNA polymerase-binding transcription factor DksA {ECO:0000255|HAMAP-Rule:MF_00926};
GN   Name=dksA {ECO:0000255|HAMAP-Rule:MF_00926}; OrderedLocusNames=BU198;
OS   Buchnera aphidicola subsp. Acyrthosiphon pisum (strain APS) (Acyrthosiphon
OS   pisum symbiotic bacterium).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Erwiniaceae; Buchnera.
OX   NCBI_TaxID=107806;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=APS;
RX   PubMed=10993077; DOI=10.1038/35024074;
RA   Shigenobu S., Watanabe H., Hattori M., Sakaki Y., Ishikawa H.;
RT   "Genome sequence of the endocellular bacterial symbiont of aphids Buchnera
RT   sp. APS.";
RL   Nature 407:81-86(2000).
CC   -!- FUNCTION: Transcription factor that acts by binding directly to the RNA
CC       polymerase (RNAP). Required for negative regulation of rRNA expression
CC       and positive regulation of several amino acid biosynthesis promoters.
CC       Also required for regulation of fis expression. {ECO:0000255|HAMAP-
CC       Rule:MF_00926}.
CC   -!- SUBUNIT: Interacts directly with the RNA polymerase.
CC       {ECO:0000255|HAMAP-Rule:MF_00926}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00926}.
CC   -!- SIMILARITY: Belongs to the DksA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00926}.
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DR   EMBL; BA000003; BAB12915.1; -; Genomic_DNA.
DR   RefSeq; NP_240029.1; NC_002528.1.
DR   RefSeq; WP_010895996.1; NC_002528.1.
DR   AlphaFoldDB; P57294; -.
DR   SMR; P57294; -.
DR   STRING; 107806.10038880; -.
DR   EnsemblBacteria; BAB12915; BAB12915; BAB12915.
DR   KEGG; buc:BU198; -.
DR   PATRIC; fig|107806.10.peg.209; -.
DR   eggNOG; COG1734; Bacteria.
DR   HOGENOM; CLU_043144_2_0_6; -.
DR   OMA; KKGEEYM; -.
DR   Proteomes; UP000001806; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0010468; P:regulation of gene expression; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00926; DksA; 1.
DR   InterPro; IPR012784; DksA_RNA_pol-bd.
DR   InterPro; IPR037187; DnaK_N.
DR   InterPro; IPR000962; Znf_DskA_TraR.
DR   InterPro; IPR020458; Znf_DskA_TraR_CS.
DR   Pfam; PF01258; zf-dskA_traR; 1.
DR   SUPFAM; SSF109635; SSF109635; 1.
DR   TIGRFAMs; TIGR02420; dksA; 1.
DR   PROSITE; PS01102; ZF_DKSA_1; 1.
DR   PROSITE; PS51128; ZF_DKSA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT   CHAIN           1..151
FT                   /note="RNA polymerase-binding transcription factor DksA"
FT                   /id="PRO_0000187542"
FT   ZN_FING         114..138
FT                   /note="dksA C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         114
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         117
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         135
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         138
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
SQ   SEQUENCE   151 AA;  17799 MW;  3CE770306C9A18E7 CRC64;
     MEKEKNKKTS SLNVLSIAGL QPYQKKIDEE YMNKDQILHF YKILETWKTQ LQDEINHTLL
     YIQDNATNFP DPIDRATQEE EFSLDLRNRD RSRKLIKKIE ITLKKIKEKD FGYCNSCSVE
     IGIRRLEARP TADLCIDCKT LAEIREKQMT G