ID   DKSA_HAEIN              Reviewed;         145 AA.
AC   P43758;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=RNA polymerase-binding transcription factor DksA {ECO:0000255|HAMAP-Rule:MF_00926};
GN   Name=dksA {ECO:0000255|HAMAP-Rule:MF_00926}; Synonyms=dsh-1;
GN   OrderedLocusNames=HI_0062;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-67.
RC   STRAIN=BC200;
RX   PubMed=8063112; DOI=10.1016/0378-1119(94)90840-0;
RA   Clifton S.W., McCarthy D., Roe B.A.;
RT   "Sequence of the rec-2 locus of Haemophilus influenzae: homologies to comE-
RT   ORF3 of Bacillus subtilis and msbA of Escherichia coli.";
RL   Gene 146:95-100(1994).
CC   -!- FUNCTION: Transcription factor that acts by binding directly to the RNA
CC       polymerase (RNAP). Required for negative regulation of rRNA expression
CC       and positive regulation of several amino acid biosynthesis promoters.
CC       Also required for regulation of fis expression. {ECO:0000255|HAMAP-
CC       Rule:MF_00926}.
CC   -!- SUBUNIT: Interacts directly with the RNA polymerase.
CC       {ECO:0000255|HAMAP-Rule:MF_00926}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00926}.
CC   -!- SIMILARITY: Belongs to the DksA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00926}.
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DR   EMBL; L42023; AAC21740.1; -; Genomic_DNA.
DR   EMBL; L20805; AAC13732.1; -; Genomic_DNA.
DR   PIR; A64046; A64046.
DR   RefSeq; NP_438235.1; NC_000907.1.
DR   RefSeq; WP_005630220.1; NC_000907.1.
DR   AlphaFoldDB; P43758; -.
DR   SMR; P43758; -.
DR   STRING; 71421.HI_0062; -.
DR   PRIDE; P43758; -.
DR   DNASU; 950956; -.
DR   EnsemblBacteria; AAC21740; AAC21740; HI_0062.
DR   KEGG; hin:HI_0062; -.
DR   PATRIC; fig|71421.8.peg.62; -.
DR   eggNOG; COG1734; Bacteria.
DR   HOGENOM; CLU_043144_2_0_6; -.
DR   OMA; KKGEEYM; -.
DR   PhylomeDB; P43758; -.
DR   BioCyc; HINF71421:G1GJ1-63-MON; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0010468; P:regulation of gene expression; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00926; DksA; 1.
DR   InterPro; IPR012784; DksA_RNA_pol-bd.
DR   InterPro; IPR037187; DnaK_N.
DR   InterPro; IPR000962; Znf_DskA_TraR.
DR   InterPro; IPR020458; Znf_DskA_TraR_CS.
DR   Pfam; PF01258; zf-dskA_traR; 1.
DR   SUPFAM; SSF109635; SSF109635; 1.
DR   TIGRFAMs; TIGR02420; dksA; 1.
DR   PROSITE; PS01102; ZF_DKSA_1; 1.
DR   PROSITE; PS51128; ZF_DKSA_2; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Metal-binding; Reference proteome; Zinc; Zinc-finger.
FT   CHAIN           1..145
FT                   /note="RNA polymerase-binding transcription factor DksA"
FT                   /id="PRO_0000187546"
FT   ZN_FING         108..132
FT                   /note="dksA C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         108
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         111
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         129
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         132
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
SQ   SEQUENCE   145 AA;  16864 MW;  ABF3EDB3CCE2733F CRC64;
     MSRASLSLLD LAGVKPYQMK KDEEYMNEEQ ILHFRKILNA WHEQIVEEAS RTVAHMQDEV
     TNFPDPADRA TQEEEFSLEL RNRDRERKLM KKIEATLKKL DTDDFGYCDC CGEEIGIRRL
     EARPTADLCI DCKTLAEIRE KQVAG