ID   DKSA_SHIFL              Reviewed;         151 AA.
AC   P0ABS4; P18274;
DT   25-OCT-2005, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2005, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=RNA polymerase-binding transcription factor DksA {ECO:0000255|HAMAP-Rule:MF_00926};
GN   Name=dksA {ECO:0000255|HAMAP-Rule:MF_00926};
GN   OrderedLocusNames=SF0137, S0140;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: Transcription factor that acts by binding directly to the RNA
CC       polymerase (RNAP). Required for negative regulation of rRNA expression
CC       and positive regulation of several amino acid biosynthesis promoters.
CC       Also required for regulation of fis expression. {ECO:0000255|HAMAP-
CC       Rule:MF_00926}.
CC   -!- SUBUNIT: Interacts directly with the RNA polymerase.
CC       {ECO:0000255|HAMAP-Rule:MF_00926}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00926}.
CC   -!- SIMILARITY: Belongs to the DksA family. {ECO:0000255|HAMAP-
CC       Rule:MF_00926}.
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DR   EMBL; AE005674; AAN41800.2; -; Genomic_DNA.
DR   EMBL; AE014073; AAP15681.1; -; Genomic_DNA.
DR   RefSeq; NP_706093.2; NC_004337.2.
DR   RefSeq; WP_001155227.1; NZ_WPGW01000006.1.
DR   AlphaFoldDB; P0ABS4; -.
DR   SMR; P0ABS4; -.
DR   STRING; 198214.SF0137; -.
DR   PRIDE; P0ABS4; -.
DR   EnsemblBacteria; AAN41800; AAN41800; SF0137.
DR   EnsemblBacteria; AAP15681; AAP15681; S0140.
DR   GeneID; 1024482; -.
DR   GeneID; 60371850; -.
DR   GeneID; 67416218; -.
DR   KEGG; sfl:SF0137; -.
DR   KEGG; sfx:S0140; -.
DR   PATRIC; fig|198214.7.peg.154; -.
DR   HOGENOM; CLU_043144_2_0_6; -.
DR   OMA; KKGEEYM; -.
DR   OrthoDB; 1932026at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0010468; P:regulation of gene expression; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_00926; DksA; 1.
DR   InterPro; IPR012784; DksA_RNA_pol-bd.
DR   InterPro; IPR037187; DnaK_N.
DR   InterPro; IPR020460; Znf_C4-type_bac.
DR   InterPro; IPR000962; Znf_DskA_TraR.
DR   InterPro; IPR020458; Znf_DskA_TraR_CS.
DR   Pfam; PF01258; zf-dskA_traR; 1.
DR   PRINTS; PR00618; DKSAZNFINGER.
DR   SUPFAM; SSF109635; SSF109635; 1.
DR   TIGRFAMs; TIGR02420; dksA; 1.
DR   PROSITE; PS01102; ZF_DKSA_1; 1.
DR   PROSITE; PS51128; ZF_DKSA_2; 1.
PE   3: Inferred from homology;
KW   Coiled coil; Cytoplasm; Metal-binding; Reference proteome; Zinc;
KW   Zinc-finger.
FT   CHAIN           1..151
FT                   /note="RNA polymerase-binding transcription factor DksA"
FT                   /id="PRO_0000187541"
FT   ZN_FING         114..138
FT                   /note="dksA C4-type"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   COILED          33..54
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         114
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         117
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         135
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
FT   BINDING         138
FT                   /ligand="Zn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29105"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00926"
SQ   SEQUENCE   151 AA;  17528 MW;  620842DB15E066A9 CRC64;
     MQEGQNRKTS SLSILAIAGV EPYQEKPGEE YMNEAQLAHF RRILEAWRNQ LRDEVDRTVT
     HMQDEAANFP DPVDRAAQEE EFSLELRNRD RERKLIKKIE KTLKKVEDED FGYCESCGVE
     IGIRRLEARP TADLCIDCKT LAEIREKQMA G