ID   DLHH_AQUAE              Reviewed;         231 AA.
AC   O67802;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Putative carboxymethylenebutenolidase;
DE            EC=3.1.1.45;
DE   AltName: Full=Dienelactone hydrolase;
DE            Short=DLH;
GN   OrderedLocusNames=aq_1997;
OS   Aquifex aeolicus (strain VF5).
OC   Bacteria; Aquificae; Aquificales; Aquificaceae; Aquifex.
OX   NCBI_TaxID=224324;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=VF5;
RX   PubMed=9537320; DOI=10.1038/32831;
RA   Deckert G., Warren P.V., Gaasterland T., Young W.G., Lenox A.L.,
RA   Graham D.E., Overbeek R., Snead M.A., Keller M., Aujay M., Huber R.,
RA   Feldman R.A., Short J.M., Olsen G.J., Swanson R.V.;
RT   "The complete genome of the hyperthermophilic bacterium Aquifex aeolicus.";
RL   Nature 392:353-358(1998).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; AE000657; AAC07773.1; -; Genomic_DNA.
DR   PIR; F70471; F70471.
DR   RefSeq; NP_214371.1; NC_000918.1.
DR   RefSeq; WP_010881307.1; NC_000918.1.
DR   AlphaFoldDB; O67802; -.
DR   SMR; O67802; -.
DR   STRING; 224324.aq_1997; -.
DR   ESTHER; aquae-dlhh; Dienelactone_hydrolase.
DR   PRIDE; O67802; -.
DR   EnsemblBacteria; AAC07773; AAC07773; aq_1997.
DR   KEGG; aae:aq_1997; -.
DR   eggNOG; COG0412; Bacteria.
DR   HOGENOM; CLU_054590_7_2_0; -.
DR   InParanoid; O67802; -.
DR   OMA; HIKAYVV; -.
DR   OrthoDB; 1889569at2; -.
DR   Proteomes; UP000000798; Chromosome.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..231
FT                   /note="Putative carboxymethylenebutenolidase"
FT                   /id="PRO_0000161576"
FT   ACT_SITE        118
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        167
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        199
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   231 AA;  26357 MW;  8268DA32A6980751 CRC64;
     MGQMVEFEKN GVKVRGYIAT PKWAGPAVLV FHEWWGLESP LSNIKEICDK LADEGFVAFA
     PDFYKGQYAD NPDDAGKLMT EMFEKRMDEV DRIFQASVEF VKECRYTYPK KVGITGFCCG
     GTLAMYFAAK FPEMVDASLP FYGLPQLTQI NAENIKVPIF FILAEKDEFV NNDEVIDIAK
     TVWKNGVDVQ VKVFSGVTHA FLNEKREDVY DPKRACEAWE LAVNFFKTYL K