ID   DLHH_ECO57              Reviewed;         271 AA.
AC   Q8X8L4;
DT   21-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2002, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Putative carboxymethylenebutenolidase;
DE            EC=3.1.1.45;
DE   AltName: Full=Dienelactone hydrolase;
DE            Short=DLH;
GN   Name=ysgA; OrderedLocusNames=Z5352, ECs4760;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG59026.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=BAB38183.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AE005174; AAG59026.1; ALT_INIT; Genomic_DNA.
DR   EMBL; BA000007; BAB38183.1; ALT_INIT; Genomic_DNA.
DR   PIR; F86070; F86070.
DR   PIR; H91223; H91223.
DR   RefSeq; NP_312787.2; NC_002695.1.
DR   RefSeq; WP_001301462.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; Q8X8L4; -.
DR   SMR; Q8X8L4; -.
DR   STRING; 155864.EDL933_5152; -.
DR   ESTHER; ecoli-dlhh; Dienelactone_hydrolase.
DR   EnsemblBacteria; AAG59026; AAG59026; Z5352.
DR   EnsemblBacteria; BAB38183; BAB38183; ECs_4760.
DR   GeneID; 915144; -.
DR   KEGG; ece:Z5352; -.
DR   KEGG; ecs:ECs_4760; -.
DR   PATRIC; fig|386585.9.peg.4969; -.
DR   eggNOG; COG0412; Bacteria.
DR   HOGENOM; CLU_054590_7_0_6; -.
DR   OMA; HIKAYVV; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..271
FT                   /note="Putative carboxymethylenebutenolidase"
FT                   /id="PRO_0000161579"
FT   ACT_SITE        147
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        204
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        236
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   271 AA;  29396 MW;  A2FCEACBDC8F91E5 CRC64;
     MATTQQSGFA PAASPLASTI VQTPDDAIVA GFTSIPSQGD NMPAYHARPK QSDGPLPVVI
     VVQEIFGVHE HIRDICRRLA LEGYLAIAPE LYFREGDPND FADIPTLLSG LVAKVPDSQV
     LADLDHVASW ASRNGGDVHR LMITGFCWGG RITWLYAAHN PQLKAAVAWY GKLTGDKSLN
     SPKQPVDIAT DLNAPVLGLY GGLDNSIPQE SVETMRQALR AANAKAEIIV YPDAGHAFNA
     DYRPSYHAAS AEDGWQRMLE WFKQYGGKKS L