ID   DLHH_ECOLI              Reviewed;         271 AA.
AC   P56262; Q2M8D5; Q6BEY8;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 3.
DT   03-AUG-2022, entry version 125.
DE   RecName: Full=Putative carboxymethylenebutenolidase;
DE            EC=3.1.1.45;
DE   AltName: Full=Dienelactone hydrolase;
DE            Short=DLH;
GN   Name=ysgA; OrderedLocusNames=b3830, JW5853;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [2]
RP   SEQUENCE REVISION.
RX   PubMed=16397293; DOI=10.1093/nar/gkj405;
RA   Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA   Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA   Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA   Thomson N.R., Wishart D., Wanner B.L.;
RT   "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT   -- 2005.";
RL   Nucleic Acids Res. 34:1-9(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; U00096; AAT48226.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77471.1; -; Genomic_DNA.
DR   RefSeq; WP_001336442.1; NZ_SSZK01000046.1.
DR   RefSeq; YP_026268.1; NC_000913.3.
DR   AlphaFoldDB; P56262; -.
DR   SMR; P56262; -.
DR   BioGRID; 4259478; 32.
DR   DIP; DIP-12929N; -.
DR   STRING; 511145.b3830; -.
DR   ESTHER; ecoli-dlhh; Dienelactone_hydrolase.
DR   jPOST; P56262; -.
DR   PaxDb; P56262; -.
DR   PRIDE; P56262; -.
DR   EnsemblBacteria; AAT48226; AAT48226; b3830.
DR   EnsemblBacteria; BAE77471; BAE77471; BAE77471.
DR   GeneID; 948320; -.
DR   KEGG; ecj:JW5853; -.
DR   KEGG; eco:b3830; -.
DR   PATRIC; fig|511145.12.peg.3946; -.
DR   EchoBASE; EB4067; -.
DR   eggNOG; COG0412; Bacteria.
DR   HOGENOM; CLU_054590_7_0_6; -.
DR   InParanoid; P56262; -.
DR   OMA; HIKAYVV; -.
DR   PhylomeDB; P56262; -.
DR   BioCyc; EcoCyc:G7804-MON; -.
DR   PRO; PR:P56262; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..271
FT                   /note="Putative carboxymethylenebutenolidase"
FT                   /id="PRO_0000161578"
FT   ACT_SITE        147
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        204
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        236
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   271 AA;  29425 MW;  CD05EAD376CD794F CRC64;
     MATTQQSGFA PAASPLASTI VQTPDDAIVA GFTSIPSQGD NMPAYHARPK QSDGPLPVVI
     VVQEIFGVHE HIRDICRRLA LEGYLAIAPE LYFREGDPND FADIPTLLSG LVAKVPDSQV
     LADLDHVASW ASRNGGDVHR LMITGFCWGG RITWLYAAHN PQLKAAVAWY GKLTGDKSLN
     SPKQPVDIAT DLNAPILGLY GGQDNSIPQE SVETMRQALR AANAKAEIIV YPDAGHAFNA
     DYRPSYHAAS AEDGWQRMLE WFKQYGGKKS L