ID   DLHH_METEA              Reviewed;         291 AA.
AC   P71505; C5B115;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Putative carboxymethylenebutenolidase;
DE            EC=3.1.1.45;
DE   AltName: Full=Dienelactone hydrolase;
DE            Short=DLH;
DE   Flags: Precursor;
GN   OrderedLocusNames=MexAM1_META1p1735;
OS   Methylorubrum extorquens (strain ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB
OS   9133 / AM1) (Methylobacterium extorquens).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Methylobacteriaceae; Methylorubrum.
OX   NCBI_TaxID=272630;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=9168622; DOI=10.1099/00221287-143-5-1729;
RA   Chistoserdova L.V., Lidstrom M.E.;
RT   "Molecular and mutational analysis of a DNA region separating two
RT   methylotrophy gene clusters in Methylobacterium extorquens AM1.";
RL   Microbiology 143:1729-1736(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1;
RX   PubMed=19440302; DOI=10.1371/journal.pone.0005584;
RA   Vuilleumier S., Chistoserdova L., Lee M.-C., Bringel F., Lajus A., Zhou Y.,
RA   Gourion B., Barbe V., Chang J., Cruveiller S., Dossat C., Gillett W.,
RA   Gruffaz C., Haugen E., Hourcade E., Levy R., Mangenot S., Muller E.,
RA   Nadalig T., Pagni M., Penny C., Peyraud R., Robinson D.G., Roche D.,
RA   Rouy Z., Saenampechek C., Salvignol G., Vallenet D., Wu Z., Marx C.J.,
RA   Vorholt J.A., Olson M.V., Kaul R., Weissenbach J., Medigue C.,
RA   Lidstrom M.E.;
RT   "Methylobacterium genome sequences: a reference blueprint to investigate
RT   microbial metabolism of C1 compounds from natural and industrial sources.";
RL   PLoS ONE 4:E5584-E5584(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; U72662; AAB58886.1; -; Genomic_DNA.
DR   EMBL; CP001510; ACS39579.1; -; Genomic_DNA.
DR   RefSeq; WP_003597626.1; NC_012808.1.
DR   AlphaFoldDB; P71505; -.
DR   SMR; P71505; -.
DR   STRING; 272630.MexAM1_META1p1735; -.
DR   ESTHER; metex-dlhh; Dienelactone_hydrolase.
DR   EnsemblBacteria; ACS39579; ACS39579; MexAM1_META1p1735.
DR   KEGG; mea:Mex_1p1735; -.
DR   eggNOG; COG0412; Bacteria.
DR   HOGENOM; CLU_054590_7_0_5; -.
DR   OMA; HIKAYVV; -.
DR   OrthoDB; 1889569at2; -.
DR   Proteomes; UP000009081; Chromosome.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Signal.
FT   SIGNAL          1..40
FT                   /evidence="ECO:0000255"
FT   CHAIN           41..291
FT                   /note="Putative carboxymethylenebutenolidase"
FT                   /id="PRO_0000007247"
FT   ACT_SITE        170
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        227
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        259
FT                   /evidence="ECO:0000250"
FT   CONFLICT        125
FT                   /note="N -> D (in Ref. 1; AAB58886)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        140
FT                   /note="A -> D (in Ref. 1; AAB58886)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   291 AA;  31053 MW;  6A51DF49220B8A98 CRC64;
     MTAFDADLRS LAAQTTLSRR TVIATSLATG FALAVQPVAA QTTIATDANG LIAGEVKIPM
     QDGVIPAYRA MPAEGGPFPT ILVVQEIFGV HEHIKDVCRR LAKLGYFALA PELYARQGDV
     STLTNIQQIV SEVVSKVPDA QVMSDLDAAV AFAKGTGKAD TARLGITGFC WGGRITWLYA
     AHNPAVKAGV AWYGRLVGDS SALMPKNPVD VAADLKAPVL GLYGGADQGI PVATIDRMKE
     ACRAAGKTCD FVVYPEAGHA FHADYRPSYR AEPAQDGWKR LQDWFRQYGV A