ID   DLHH_SALTI              Reviewed;         270 AA.
AC   Q8Z3B8;
DT   21-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2002, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Putative carboxymethylenebutenolidase;
DE            EC=3.1.1.45;
DE   AltName: Full=Dienelactone hydrolase;
DE            Short=DLH;
GN   Name=ysgA; OrderedLocusNames=STY3592, t3330;
OS   Salmonella typhi.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=90370;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CT18;
RX   PubMed=11677608; DOI=10.1038/35101607;
RA   Parkhill J., Dougan G., James K.D., Thomson N.R., Pickard D., Wain J.,
RA   Churcher C.M., Mungall K.L., Bentley S.D., Holden M.T.G., Sebaihia M.,
RA   Baker S., Basham D., Brooks K., Chillingworth T., Connerton P., Cronin A.,
RA   Davis P., Davies R.M., Dowd L., White N., Farrar J., Feltwell T.,
RA   Hamlin N., Haque A., Hien T.T., Holroyd S., Jagels K., Krogh A.,
RA   Larsen T.S., Leather S., Moule S., O'Gaora P., Parry C., Quail M.A.,
RA   Rutherford K.M., Simmonds M., Skelton J., Stevens K., Whitehead S.,
RA   Barrell B.G.;
RT   "Complete genome sequence of a multiple drug resistant Salmonella enterica
RT   serovar Typhi CT18.";
RL   Nature 413:848-852(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700931 / Ty2;
RX   PubMed=12644504; DOI=10.1128/jb.185.7.2330-2337.2003;
RA   Deng W., Liou S.-R., Plunkett G. III, Mayhew G.F., Rose D.J., Burland V.,
RA   Kodoyianni V., Schwartz D.C., Blattner F.R.;
RT   "Comparative genomics of Salmonella enterica serovar Typhi strains Ty2 and
RT   CT18.";
RL   J. Bacteriol. 185:2330-2337(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; AL513382; CAD07925.1; -; Genomic_DNA.
DR   EMBL; AE014613; AAO70858.1; -; Genomic_DNA.
DR   RefSeq; NP_457784.1; NC_003198.1.
DR   AlphaFoldDB; Q8Z3B8; -.
DR   SMR; Q8Z3B8; -.
DR   STRING; 220341.16504470; -.
DR   ESTHER; salty-DLHH; Dienelactone_hydrolase.
DR   PRIDE; Q8Z3B8; -.
DR   EnsemblBacteria; AAO70858; AAO70858; t3330.
DR   KEGG; stt:t3330; -.
DR   KEGG; sty:STY3592; -.
DR   PATRIC; fig|220341.7.peg.3659; -.
DR   eggNOG; COG0412; Bacteria.
DR   HOGENOM; CLU_054590_7_0_6; -.
DR   OMA; HIKAYVV; -.
DR   Proteomes; UP000000541; Chromosome.
DR   Proteomes; UP000002670; Chromosome.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..270
FT                   /note="Putative carboxymethylenebutenolidase"
FT                   /id="PRO_0000161580"
FT   ACT_SITE        147
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        204
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        236
FT                   /evidence="ECO:0000250"
FT   CONFLICT        201
FT                   /note="S -> G (in Ref. 2; AAO70858)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   270 AA;  29092 MW;  0D7630B6046075B2 CRC64;
     MTTTHPSGFA PAASPLAPTM IHTPDGAISA GITSIPSQGD DMPAYYARPK ASDGALPVVI
     VVQEIFGVHE HIRDICRRLA LEGYLAIAPE LYFREGDPND FADIPTLLSG LVAKVPDSQV
     LADLDHVASW ASRNGGDAHR LMITGFCWGG RITWLYAAHN PQLKAAVAWY GKLVGDTSLN
     SPKHPVDIAT DLNAPVLGLY SGQDTSIPQE SVETMRQALR AANAKAEIVV YPDAGHAFNA
     DYRPGYHEAS AKDGWQRMLE WFAQYGGKKG