ID   DLHH_SALTY              Reviewed;         270 AA.
AC   Q9L6M9;
DT   21-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Putative carboxymethylenebutenolidase;
DE            EC=3.1.1.45;
DE   AltName: Full=Dienelactone hydrolase;
DE            Short=DLH;
GN   Name=ysgA; OrderedLocusNames=STM3967; ORFNames=STMD1.22;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; AF233324; AAF33425.1; -; Genomic_DNA.
DR   EMBL; AE006468; AAL22811.1; -; Genomic_DNA.
DR   RefSeq; NP_462852.1; NC_003197.2.
DR   RefSeq; WP_000213959.1; NC_003197.2.
DR   AlphaFoldDB; Q9L6M9; -.
DR   SMR; Q9L6M9; -.
DR   STRING; 99287.STM3967; -.
DR   ESTHER; salty-DLHH; Dienelactone_hydrolase.
DR   PaxDb; Q9L6M9; -.
DR   EnsemblBacteria; AAL22811; AAL22811; STM3967.
DR   GeneID; 1255493; -.
DR   KEGG; stm:STM3967; -.
DR   PATRIC; fig|99287.12.peg.4186; -.
DR   HOGENOM; CLU_054590_7_0_6; -.
DR   OMA; HIKAYVV; -.
DR   PhylomeDB; Q9L6M9; -.
DR   BioCyc; SENT99287:STM3967-MON; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..270
FT                   /note="Putative carboxymethylenebutenolidase"
FT                   /id="PRO_0000161581"
FT   ACT_SITE        147
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        204
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        236
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   270 AA;  29062 MW;  1C2374B6047DA872 CRC64;
     MTTTHPSGFA PAASPLAPTM IHTPDGAISA GITSIPSQGD DMPAYYARPK ASDGALPVVI
     VVQEIFGVHE HIRDICRRLA LEGYLAIAPE LYFREGDPND FADIPTLLSG LVAKVPDSQV
     LADLDHVASW ASRNGGDAHR LMITGFCWGG RITWLYAAHN PQLKAAVAWY GKLVGDTSLN
     SPKHPVDIAT DLNAPVLGLY GGQDTSIPQE SVETMRQALR AANAKAEIVV YPDAGHAFNA
     DYRPGYHEAS AKDGWQRMLE WFAQYGGKKG