DLHH_YEAST
ID DLHH_YEAST Reviewed; 273 AA.
AC Q07505; D6VRR2;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Putative carboxymethylenebutenolidase;
DE EC=3.1.1.45;
DE AltName: Full=Dienelactone hydrolase;
DE Short=DLH;
GN OrderedLocusNames=YDL086W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC 2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC -!- INTERACTION:
CC Q07505; P33203: PRP40; NbExp=2; IntAct=EBI-5951, EBI-701;
CC -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC {ECO:0000305}.
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DR EMBL; Z74134; CAA98652.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11772.1; -; Genomic_DNA.
DR PIR; S67622; S67622.
DR RefSeq; NP_010197.1; NM_001180145.1.
DR AlphaFoldDB; Q07505; -.
DR SMR; Q07505; -.
DR BioGRID; 31974; 51.
DR DIP; DIP-3879N; -.
DR IntAct; Q07505; 8.
DR MINT; Q07505; -.
DR STRING; 4932.YDL086W; -.
DR ESTHER; yeast-dlhh; Dienelactone_hydrolase.
DR MaxQB; Q07505; -.
DR PaxDb; Q07505; -.
DR PRIDE; Q07505; -.
DR EnsemblFungi; YDL086W_mRNA; YDL086W; YDL086W.
DR GeneID; 851472; -.
DR KEGG; sce:YDL086W; -.
DR SGD; S000002244; YDL086W.
DR VEuPathDB; FungiDB:YDL086W; -.
DR eggNOG; ENOG502QQFN; Eukaryota.
DR HOGENOM; CLU_054590_1_0_1; -.
DR InParanoid; Q07505; -.
DR OMA; CATCFFP; -.
DR BioCyc; YEAST:YDL086W-MON; -.
DR PRO; PR:Q07505; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q07505; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0008806; F:carboxymethylenebutenolidase activity; ISS:SGD.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002925; Dienelactn_hydro.
DR Pfam; PF01738; DLH; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 1: Evidence at protein level;
KW Hydrolase; Reference proteome.
FT CHAIN 1..273
FT /note="Putative carboxymethylenebutenolidase"
FT /id="PRO_0000161585"
FT ACT_SITE 130
FT /evidence="ECO:0000250"
FT ACT_SITE 191
FT /evidence="ECO:0000250"
FT ACT_SITE 223
FT /evidence="ECO:0000250"
SQ SEQUENCE 273 AA; 30837 MW; 9A4A9B7577243D46 CRC64;
MLITETFHDV QTSYGTTLRI YVYSPKIAGY PQAKFPGVIL YSEIYQVTGP VRRFGQRIAS
EGYVVVAPAI YHNFMGPEAL PYDVQGTDIG NEYKIKKPLE SYDEDNKLCC DLLFQLPQFD
GKRIGSTGMC LGGHLAFRAL LDKRVTCATC FFPTDIHSRT LGLGQNDNSL ERVSKELGNN
QEMVLIFGTA DTHVDPQGRD LIRKTLRDHG VKFTFLEILA AQHAFIRDEF SKGRFDSAIT
QSCLGFLFEQ FNRKLRIDLG EFVDDNTPLE HVC
