ID   DLHH_YERPE              Reviewed;         267 AA.
AC   Q8ZAL4; Q0WAM4;
DT   21-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2002, sequence version 1.
DT   25-MAY-2022, entry version 108.
DE   RecName: Full=Putative carboxymethylenebutenolidase;
DE            EC=3.1.1.45;
DE   AltName: Full=Dienelactone hydrolase;
DE            Short=DLH;
GN   OrderedLocusNames=YPO3787, y0443, YP_3262;
OS   Yersinia pestis.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=632;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CO-92 / Biovar Orientalis;
RX   PubMed=11586360; DOI=10.1038/35097083;
RA   Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA   Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA   Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA   Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA   Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA   Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA   Stevens K., Whitehead S., Barrell B.G.;
RT   "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL   Nature 413:523-527(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KIM10+ / Biovar Mediaevalis;
RX   PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA   Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA   Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA   Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA   Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT   "Genome sequence of Yersinia pestis KIM.";
RL   J. Bacteriol. 184:4601-4611(2002).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=91001 / Biovar Mediaevalis;
RX   PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA   Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA   Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA   Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA   Wang J., Huang P., Yang R.;
RT   "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT   avirulent to humans.";
RL   DNA Res. 11:179-197(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(5-oxo-2,5-dihydrofuran-2-ylidene)acetate + H2O = 4-oxohex-
CC         2-enedioate + H(+); Xref=Rhea:RHEA:12372, ChEBI:CHEBI:12040,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:57263; EC=3.1.1.45;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAM84032.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC       Sequence=AAS63430.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; AL590842; CAL22373.1; -; Genomic_DNA.
DR   EMBL; AE009952; AAM84032.1; ALT_INIT; Genomic_DNA.
DR   EMBL; AE017042; AAS63430.1; ALT_INIT; Genomic_DNA.
DR   PIR; AB0461; AB0461.
DR   RefSeq; YP_002348664.1; NC_003143.1.
DR   AlphaFoldDB; Q8ZAL4; -.
DR   SMR; Q8ZAL4; -.
DR   STRING; 214092.YPO3787; -.
DR   ESTHER; yerpe-dlhh; Dienelactone_hydrolase.
DR   PaxDb; Q8ZAL4; -.
DR   DNASU; 1145390; -.
DR   EnsemblBacteria; AAM84032; AAM84032; y0443.
DR   EnsemblBacteria; AAS63430; AAS63430; YP_3262.
DR   KEGG; ype:YPO3787; -.
DR   KEGG; ypk:y0443; -.
DR   KEGG; ypm:YP_3262; -.
DR   PATRIC; fig|214092.21.peg.4309; -.
DR   eggNOG; COG0412; Bacteria.
DR   HOGENOM; CLU_054590_7_0_6; -.
DR   OMA; HIKAYVV; -.
DR   Proteomes; UP000000815; Chromosome.
DR   Proteomes; UP000001019; Chromosome.
DR   Proteomes; UP000002490; Chromosome.
DR   GO; GO:0008806; F:carboxymethylenebutenolidase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Reference proteome.
FT   CHAIN           1..267
FT                   /note="Putative carboxymethylenebutenolidase"
FT                   /id="PRO_0000161583"
FT   ACT_SITE        137
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        194
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        226
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   267 AA;  29671 MW;  F10398E0A1ABF1AB CRC64;
     MATAHHTDQL HSLQTHGIHC GETTIPSQGD ELPAYIAKPD QHTGPYPVVI VVQEIFGVHE
     HIQDICRRLA KQGYLAIAPE LYFRQGDAKD YSNINELVNN LVKKVPDRQV LVDLDHTAHW
     ASRHGGDTKK LAITGFCWGG RIAWLYAAHN PQLKAAVAWY GKLVGEKTLF LPKYPVDVAI
     DLCAPVLGLY GGKDTSIPAE HIETMRQALR AANADAEIIV YPEAGHAFNA DYRPSYHAES
     AQDGWQRMLD WFTQHGVSAI PIPEETQ