DLPB_DICDI
ID DLPB_DICDI Reviewed; 808 AA.
AC Q54MH8;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Dynamin-like protein B;
DE EC=3.6.5.5;
GN Name=dlpB; ORFNames=DDB_G0285931;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, AND DEVELOPMENTAL STAGE.
RX PubMed=18809930; DOI=10.1073/pnas.0802412105;
RA Miyagishima S.Y., Kuwayama H., Urushihara H., Nakanishi H.;
RT "Evolutionary linkage between eukaryotic cytokinesis and chloroplast
RT division by dynamin proteins.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:15202-15207(2008).
CC -!- FUNCTION: Involved in cytokinesis. May hydrolyze GTP.
CC {ECO:0000269|PubMed:18809930}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; EC=3.6.5.5;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expression begins during cell cycle progression,
CC between 12 and 24 hours after germination. Reach a maximum around mid-
CC log phase and disappear during the stationary phase.
CC {ECO:0000269|PubMed:18809930}.
CC -!- DISRUPTION PHENOTYPE: Produced cells are larger and a large amount of
CC them contains more than 2 nuclei. {ECO:0000269|PubMed:18809930}.
CC -!- SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase
CC superfamily. Dynamin/Fzo/YdjA family. {ECO:0000255|PROSITE-
CC ProRule:PRU01055}.
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DR EMBL; AAFI02000082; EAL64500.1; -; Genomic_DNA.
DR RefSeq; XP_638012.1; XM_632920.1.
DR AlphaFoldDB; Q54MH8; -.
DR SMR; Q54MH8; -.
DR STRING; 44689.DDB0302371; -.
DR PaxDb; Q54MH8; -.
DR EnsemblProtists; EAL64500; EAL64500; DDB_G0285931.
DR GeneID; 8625363; -.
DR KEGG; ddi:DDB_G0285931; -.
DR dictyBase; DDB_G0285931; dlpB.
DR eggNOG; KOG0446; Eukaryota.
DR HOGENOM; CLU_348987_0_0_1; -.
DR InParanoid; Q54MH8; -.
DR OMA; KAYNKIM; -.
DR PhylomeDB; Q54MH8; -.
DR BRENDA; 3.6.5.5; 1939.
DR PRO; PR:Q54MH8; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0032154; C:cleavage furrow; IDA:dictyBase.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IBA:GO_Central.
DR GO; GO:0007015; P:actin filament organization; IGI:dictyBase.
DR GO; GO:0061952; P:midbody abscission; IMP:dictyBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:dictyBase.
DR GO; GO:0006997; P:nucleus organization; IMP:dictyBase.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR022812; Dynamin.
DR InterPro; IPR045063; Dynamin_N.
DR InterPro; IPR030381; G_DYNAMIN_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR11566; PTHR11566; 1.
DR Pfam; PF00350; Dynamin_N; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS51718; G_DYNAMIN_2; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Cytoplasm; GTP-binding; Hydrolase;
KW Motor protein; Nucleotide-binding; Reference proteome.
FT CHAIN 1..808
FT /note="Dynamin-like protein B"
FT /id="PRO_0000371338"
FT DOMAIN 43..340
FT /note="Dynamin-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 53..60
FT /note="G1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 79..80
FT /note="G2 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 150..153
FT /note="G3 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 239..242
FT /note="G4 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 276..279
FT /note="G5 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01055"
FT REGION 536..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 665..695
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 541..565
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 53..60
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 150..154
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
FT BINDING 239..242
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000255"
SQ SEQUENCE 808 AA; 92714 MW; 4F0410125AA5ED09 CRC64;
MDRQSVVKST AIPFPLNFDN EKYDDMYKAY NKIMVLARDL NAFIETPEFV FIGKDGNGKS
ALIESFIGFP MMIGEGSSLR PLHITLMNNA RCEEPIVTFK RDRSLDSYEF DRQIELSMVS
SEISKRNQKT SIPIEITIEY RYYLNMLLIE PPSVSIQPTN AITIQGQSMT SPANQLANKI
AKLSIGNEMG EMITQYTKSN NRTLVFVETS TNGGTNSSEM LELAKKLDYK LDRSIFVFNK
FHSLLTGDQP FTNGRDANRF LGSPSIGAPT FFTTLPSTAQ RSQCNSKDQL SQLCDQLQQT
DLNILEQLQF DKKYERNVGL SAFRHWISEF TWRKYLDSVP EVLKRLNSFR TTSEDQLYQI
RQQLERTNAV TLRQIANSYV SIEFIQCIEK LVTRTLEGNP SLNGQTLEEE KSQDETGDWY
DHNGKPILLL NDEKLVTFYD NKLYGGQQFE RLLTEFKCIT EVIQLEELSI SEVACAIGSN
RPSNASVIAW AASDLAQKKI KEALLPLVDQ LFKRATYILR RLVDIVDRMI ENKKKSSFRR
HGNSTSLFQD NSSPSSQSQS QSSSISQSAS LSSENMIYGI QSINGSDSVS RPSHENTIVN
VEDHPYFIYS VKEMYFKYVD QIAADCKNKC MDEFYTTRLI YWDLQSNKDL KKFCTDSPCV
SLNNSLNNNN KSTTPGNNNN NNNNNSNNNY NNSNHILNPK ETHTMVTELA SKLFQDIRNR
MSKNIMLKCY NYFLIPMQMD LKLNIQDNIT KLSDAMLEEI FEIQTTKERL REDEQHLAQI
CNQFIQQEEN YKKYSQSFSH PFPSAVRN
